ABSTRACT
This work studies a series of strategies in the production of lutein by Scenedesmus obliquus CWL-1 under mixotrophic cultivation. Our experimental results revealed that the optimal conditions associated with light-related strategies were 12â¯h light period followed by a 12â¯h dark period and blue to red light under mixotrophic cultivation. Under such conditions, the biomass, lutein content and lutein productivity were maximized to 9.88â¯(g/L), 1.78â¯(mg/g) and 1.43â¯(mg/L/day), respectively. Moreover, the assimilation of 4.5â¯g/L of calcium nitrate into S. obliquus CWL-1 increased the maximal biomass (12.73â¯g/L) and the highest maximal lutein productivity (3.06â¯mg/L/day), while the assimilation of 1.5â¯g/L of calcium nitrate yielded the highest maximal lutein content of 2.45â¯mg/g. The highest maximal lutein productivity of 4.96â¯(mg/L/day) was obtained when fed-batch fermentation was conducted, and this value was approximately 11-folds that obtained using the batch system.