Identification of TaGL1-B1 gene controlling grain length through regulation of jasmonic acid in common wheat.

Grain length is one of the most important factors in determining wheat yield. Here, a stable QTL for grain length was mapped on chromosome 1B in a F10 recombinant inbred lines (RIL) population, and the gene TaGL1-B1 encoding carotenoid isomerase was identified in a secondary large population through multiple strategies. The genome-wide association study (GWAS) in 243 wheat accessions revealed that the marker for TaGL1-B1 was the most significant among all chromosomes. EMS mutants of TaGL1 possessed significantly reduced grain length, whereas TaGL1-B1-overexpressed lines possessed significantly increased grain length. Moreover, TaGL1-B1 strongly interacted with TaPAP6. TaPAP6-overexpressed lines had significantly increased grain length. Transcriptome analysis suggested that TaPAP6 was possibly involved in the accumulation of JA (jasmonic acid). Consistently, JA content was significantly increased in the TaGL1-B1 and TaPAP6 overexpression lines. Additionally, the role of TaGL1-B1 in regulating carotenoids was verified through QTL mapping, GWAS, EMS mutants and overexpression lines. Notably, overexpression of TaGL1-B1 significantly increased wheat yield in multiple locations. Taken together, overexpression of TaGL1-B1 enhanced grain length, probably through interaction with TaPAP6 to cause the accumulation of JA that improved carotenoid content and photosynthesis, thereby resulted in increased wheat yield. This study provided valuable genes controlling grain length to improve yield and a potential insight into the molecular mechanism of modulating JA-mediated grain size in wheat.

Alternaria species in section Alternaria associated with Iris plants in China.

Plants of the Iris genus have been widely cultivated because of their medicinal, ornamental, and economic values. It commonly suffers from Alternaria leaf spot or blight disease leading to considerable losses for their commercial values. During an investigation of 14 provinces or municipalities of China from 2014 to 2022, a total of 122 Alternaria strains in section Alternaria were obtained from diseased leaves of Iris spp.. Among them, 12 representative strains were selected and identified based on morphological characterization and multi-locus phylogenetic analysis, which encompassed the internal transcribed spacer of rDNA region (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), translation elongation factor 1 alpha (TEF1), RNA polymerase second largest subunit (RPB2), Alternaria major allergen gene (Alt a 1), an anonymous gene region (OPA10-2), and endopolygalacturonase gene (EndoPG). The strains comprised two known species of A. alternata and A. iridicola, and two new species of A. setosae and A. tectorum, which were described and illustrated here. Their pathogenicity evaluated on Iris setosa indicated that all the strains could induce typical Alternaria leaf spot or blight symptoms. The results showed that the virulence was variable among those four species, from which A. tectorum sp. nov. was the most virulent one, followed by A. setosae sp. nov., A. iridicola and A. alternata.

Morphological and molecular identification of two new Alternaria species (Ascomycota, Pleosporaceae) in section Radicina from China.

The fungal genus Alternaria was distributed widely and found in different habitats such as plant or indoor environment. During an investigation into this genus in China, two new Alternaria species, Alternaria vulgarae and A. divaricatae were respectively isolated from diseased leaves of Foeniculum vulgare and Saposhnikovia divaricata, which both belonged to Umbelliferae. Phylogenetically, they were determined as new species belonging in the section Radicina of Alternaria based on the combined four gene fragments of ITS, TEF1, GAPDH and RPB2. Morphologically, the two species were illustrated and compared with other relevant Alternaria species in section Radicina.

We are what we do: research outputs of public health.

BACKGROUND: If public health research does not progress beyond descriptive research to testing the effectiveness of interventions that can provide causal data, its contribution to evidence-based practice will not be realized. This paper examined the number and percentage of public health research publications over three time periods (1987-1988, 1997-1998, 2005-2006) for three preventive health issues: tobacco use, alcohol use, and inadequate physical activity. METHODS: A computer-based literature search was conducted, using the Medline and PsycINFO databases. A random sample of 1000 abstracts for each preventive health issue was examined per time period. The abstracts were first categorized as public health or not, and then as data-based or non-data-based public health research. Data-based publications were classified according to research type as measurement-, descriptive/epidemiologic-, or intervention-oriented. Data analysis occurred in 2007. RESULTS: The number of data-based public health research publications examined for each topic ranged between 180 and 346 in 1987-1988, 199 and 322 in 1997-1998, and 302 and 364 in 2005-2006. Despite a significant increase over time in the percentage of intervention publications for tobacco (from 10% in 1987-1988 to 18% in 2005-2006, p=0.008), the majority of publications in all three time periods were descriptive/epidemiologic for all topics (62%-87% in 1987-1988, 64%-85% in 1997-1998, 78%-79% in 2005-2006). There were fewer measurement (3%-7% in 1987-1988, 2%-6% in 1997-1998, 4%-10% in 2005-2006) or intervention publications (9%-31% in 1987-1988, 10%-30% in 1997-1998, 12%-18% in 2005-2006). CONCLUSIONS: Descriptive research does not provide optimal evidence for how to reduce preventable illness. Reasons for the lack of measurement and intervention public health research are explored.

Study of drug resistant cases among new pulmonary tuberculosis patients attending a tuberculosis center, Yangon, Myanmar.

A cross-sectional descriptive study was carried out at a tuberculosis center, Yangon, Myanmar from October 2003 to July 2004 to analyze the drug susceptibility of new sputum smear positive pulmonary tuberculosis patients. A total of 202 Mycobacterium tuberculosis isolates were tested for resistance to isoniazid, streptomycin, rifampicin and ethambutol. Resistance to at least one anti-tuberculosis drug was documented in 32 (15.8%) isolates. Monoresistance (resistance to one drug) was noted in 15 (7.4%) isolates and poly-resistance (resistance to two or more drugs) was noted in 17 (9.4%) isolates, including 8 (4.0%) multi-drug resistant isolates (resistance to at least isoniazid and rifampicin). Total resistance to individual anti-tuberculosis drugs were: isoniazid (29, 14.3%), streptomycin (11, 5.4%), rifampicin (10, 4.9%) and ethambutol (1, 0.5%). The demographic data and possible contributing factors of drug resistance were evaluated among the drug resistant patients. Poly-resistant cases had significantly longer intervals between symptom appearance and achieving effective anti-tuberculosis treatment than mono-resistant cases (p = 0.015).

Pilot of a novel cervical screening intervention: interactive voice response.

OBJECTIVE: This paper describes the pilot of an interactive voice response (IVR) cervical screening brief advice interface. METHOD: In September 2000, 5,000 households within Cessnock, New South Wales, were contacted by an IVR system. The system randomly selected eligible women aged 18-69, assessed their cervical screening status and provided a tailored message about cervical screening. A computer-assisted telephone interview (CATI) evaluated intervention acceptability. Participation rates, participant demographics, IVR use by women, and IVR costs were also examined. RESULTS: 549 (12%) women listened to the IVR call and 587 (59%) completed the CATI interview. IVR listeners under-represented younger and older women, and over-represented women who were married, and of Aboriginal descent. The majority of women (98%) found IVR easy to answer and 90% agreed IVR reminders were a good idea. Unscreened and older women used the system more than screened or younger women. CONCLUSIONS: The IVR call was successfully used by a large number of women to obtain information on cervical cancer screening, with higher use by recognised risk groups. IMPLICATIONS: These results suggest that an IVR reminder might be useful to increase cervical screening rates since those most at risk also used IVR the most. The potential for linking IVR to a database to issue reminders in the same manner as Pap Test Register postal reminders needs to be explored.