ABSTRACT
We report a case of disseminated histoplasmosis, initially diagnosed from gut nodule colonoscopically, along with evidence of lung disease. Subsequently he developed Addisonian crisis due to adrenal involvement. Lessons were learnt from the importance of detailed history-personal and social/family, as well as psychosocial aspects of illness. Public health measures to reduce bird dropping (and thus possibility of histoplasmosis) are discussed.
Subject(s)
Craniocerebral Trauma/complications , Biomechanical Phenomena , Humans , Manikins , Mathematics , Motion Pictures , Rotation , TransducersABSTRACT
Normal and neoplastic mouse mammary epithelial cells were cultured in nutrient medium containing D-valine substituted for L-valine. Fibroblast overgrowth was prevented and epithelial cell functions and morphology were retained in cultures maintained in D-valine medium up to 2 months. A nonenzymatic technique was devised to dissociate epithelial cell monolayers. The combined use of this dissociation buffer and D-valine nutrient medium made it possible to passage serially normal and neoplastic mammary epithelial cells. Normal cells were derived from mammary glands of animals stimulated with exogenous hormones for various periods. The period of in vivo hormonal stimulation influenced the ability of normal mammary epithelial cells to attach and proliferate in primary and serially passaged cultures. A greater proportion of cells derived from glands following 2 to 4 weeks of hormonal stimulation were recovered after replating and showed higher labeling indices during serial passage than cells from unstimulated or 5- to 7-week stimulated groups.
Subject(s)
Epithelial Cells , Adenocarcinoma , Animals , Cell Division , Cell Line , Culture Media , Culture Techniques , Female , Mammary Glands, Animal , Mammary Neoplasms, Experimental , Mice , Stereoisomerism , Valine/metabolismSubject(s)
Acyltransferases/blood , Liposomes/metabolism , Pyrenes/pharmacology , Acylation , Acyltransferases/isolation & purification , Acyltransferases/metabolism , Ammonium Sulfate , Butanols , Chemical Phenomena , Chemistry , Cholesterol , Chromatography, Gel , Drug Stability , Fatty Acids , Fluorescence , Humans , Membranes, Artificial , Phosphatidylcholines , Phospholipids , Photochemistry , Spectrometry, Fluorescence , Structure-Activity Relationship , Surface Properties , Temperature , Thermodynamics , Ultracentrifugation , ViscositySubject(s)
Gluconates/metabolism , Glyceraldehyde/metabolism , Organophosphorus Compounds/metabolism , Pseudomonas/enzymology , Aerobiosis , Carbon Radioisotopes , Cell-Free System , Chromatography, Paper , Cyanides/pharmacology , Glutathione , Hydro-Lyases/metabolism , Kinetics , L-Lactate Dehydrogenase/metabolism , Lactates , Lyases/metabolism , Phosphoric Monoester Hydrolases/metabolism , Pseudomonas/drug effects , Spectrophotometry, Ultraviolet , Time Factors , Transferases/metabolism , UltracentrifugationSubject(s)
Glucokinase/metabolism , Pseudomonas/enzymology , Adenosine Diphosphate/pharmacology , Binding Sites , Catalysis , Chromatography, Paper , Glucokinase/antagonists & inhibitors , Hydrogen-Ion Concentration , Kinetics , Magnesium/pharmacology , Mathematics , Protein Binding , Pseudomonas/drug effects , Spectrophotometry, UltravioletABSTRACT
Two strains of Escherichia coli which produce hydrogen sulfide appear to have acquired this ability via transfer of genetic material from another genus.
