Perceived stress and mobile phone addiction among college students during the 2019 coronavirus disease: The mediating roles of rumination and the moderating role of self-control.

This present study aimed to examine the mediating role of rumination and the moderating role of self-control in the link between perceived stress and mobile phone addiction during the COVID-19 epidemic. A total of 628 college students completed Depression-Anxiety-Stress Scale, Smartphone Addiction Scale, Ruminative Responses Scale and Self-Control Scale. Mediation analysis highlighted that rumination mediated the association between perceived stress and mobile phone addiction. Moderated mediation analysis indicated that the indirect association between perceived stress and mobile phone addiction were moderated by self-control. Between the COVID affected group and the unaffected group, some differences also be observed in the moderating effect of self-control. This study emphasize the importance of rumination and self-control in understanding the possible mechanisms underlying the relationship between perceived stress and mobile phone addiction, which can be used to develop interventions to reduce the problematic behavior among college students during the COVID-19 pandemic.

Structural insights into the binding of nanobodies LaM2 and LaM4 to the red fluorescent protein mCherry.

Red fluorescent proteins (RFPs) are powerful tools used in molecular biology research. Although RFP can be easily monitored in vivo, manipulation of RFP by suitable nanobodies binding to different epitopes of RFP is still desired. Thus, it is crucial to obtain structural information on how the different nanobodies interact with RFP. Here, we determined the crystal structures of the LaM2-mCherry and LaM4-mCherry complexes at 1.4 and 1.9 Å resolution. Our results showed that LaM2 binds to the side of the mCherry ß-barrel, while LaM4 binds to the bottom of the ß-barrel. The distinct binding sites of LaM2 and LaM4 were further verified by isothermal titration calorimetry, fluorescence-based size exclusion chromatography, and dynamic light scattering assays. Mutation of the residues at the LaM2 or LaM4 binding interface to mCherry significantly decreased the binding affinity of the nanobody to mCherry. Our results also showed that LaM2 and LaM4 can bind to mCherry simultaneously, which is crucial for recruiting multiple operation elements to the RFP. The binding of LaM2 or LaM4 did not significantly change the chromophore environment of mCherry, which is important for fluorescence quantification assays, while several GFP nanobodies significantly altered the fluorescence. Our results provide atomic resolution interaction information on the binding of nanobodies LaM2 and LaM4 with mCherry, which is important for developing detection and manipulation methods for RFP-based biotechnology.

Effects of carbon availability in a woody carbon source on its nitrate removal behavior in solid-phase denitrification.

Woody biomass is the most common natural carbon source applied in solid-phase denitrification (SPD). However, its denitrification ability is low in the SPD process due to its poor carbon availability. In this study, sawdust samples were pretreated to various degrees, and then filled into SPD bioreactors to reveal the relationship between carbon availability and denitrification behaviors. The behaviors include the denitrification process, internal effects of major factors (carbon availability, pH and temperature), and the presence of bacterial communities. Results shown that the long-term denitrification rate of pretreated sawdust was increased by 4.5-4.8 times over that of untreated sawdust (29.3 mg N L-1 sawdust d-1). However, despite improving the pretreatment degree of the sawdust in the bioreactor, the long-term denitrification rate shown no further increase. The denitrification rate was most influenced by the temperature, followed by the pH, and then the sawdust pretreatment degree. The denitrification rate increased with decreasing pH and rising temperature of the pretreated sawdust. The removed nitrate was rarely converted into nitrite or nitrous oxide, but ammonium was produced at high pH and temperature for the pretreated sawdust. The adverse effects of ammonium and dissolved organic carbon (DOC) reduced when the pH of the pretreated sawdust was lowered to 6.5. Hydrolytic and denitrifying bacteria formed the main SPD bioreactor bacteria, whose abundances increased with increasing sawdust pretreatment degree. The results were beneficial to reduce the hydrolytic retention time and adverse products for the SPD system using woody carbon source.

Effects of alkali-treated agricultural residues on nitrate removal and N2O reduction of denitrification in unsaturated soil.

Lignocellulosic agricultural residues were utilized as denitrification carbon substrates to improve the purification capacity of unsaturated soil and alleviate nitrate pollution of groundwater. In this study, corncob and wheat straw were treated by calcium hydroxide to improve biodegradability and enhance denitrification potential. Calcium hydroxide treatment decreased the contents of lignin (i.e., from 16.7 wt% to 15.2 wt% in corncob and from 21.9 wt% to 20.6 wt% in wheat straw), increased potential biodegradable carbon by 4.4-5.3 times, reached complete nitrate removal 7-14 days earlier and decreased N2O/(N2O+N2) ratios by 85-99%. The results provide an insight into the application of alkali-treated agricultural residues as denitrification carbon sources to alleviate nitrate transport to groundwater and reduce potential greenhouse effect.

Selective enhancement and verification of woody biomass digestibility as a denitrification carbon source.

The denitrification efficiency of woody biomass as carbon source is low because of its poor carbon availability. In this study, representative poplar sawdust was pretreated with lime and peracetic acid to enhance the biomass digestibility to different degrees; sawdust was then mixed with soil to investigate its denitrification efficiency. Under controllable conditions (25-95°C, 12-24h, varying dosages), sawdust digestibility (characterized by reducing sugar yield) was selectively enhanced 1.0-21.8 times over that of the raw sawdust (28.8mgeq.glucoseg-1 dry biomass). This increase was mainly attributed to the removal of lignin from the biomass. As a carbon source, the sawdust (digestibility enhanced by 5.4 times) increased the nitrate removal rate by 4.7 times, without N2O emission. However, the sawdust with high digestibility (12.6 or 18.0 times), despite releasing more dissolved organic carbon (DOC), did not exhibit further increase in denitrification efficiency, and emitted N2O.

Highly Ordered Self-Assembly of Native Proteins into 1D, 2D, and 3D Structures Modulated by the Tether Length of Assembly-Inducing Ligands.

In nature, proteins self-assemble into various structures with different dimensions. To construct these nanostructures in laboratories, normally proteins with different symmetries are selected. However, most of these approaches are engineering-intensive and highly dependent on the accuracy of the protein design. Herein, we report that a simple native protein LecA assembles into one-dimensional nanoribbons and nanowires, two-dimensional nanosheets, and three-dimensional layered structures controlled mainly by small-molecule assembly-inducing ligands RnG (n=1, 2, 3, 4, 5) with varying numbers of ethylene oxide repeating units. To understand the formation mechanism of the different morphologies controlled by the small-molecule structure, molecular simulations were performed from microscopic and mesoscopic view, which presented a clear relationship between the molecular structure of the ligands and the assembled patterns. These results introduce an easy strategy to control the assembly structure and dimension, which could shed light on controlled protein assembly.