Combined exposure to decabromodiphenyl ether and nano zero-valent iron aggravated oxidative stress and interfered with metabolism in earthworms.

Decabromodiphenyl ether (BDE-209) is a common brominated flame retardant in electronic waste, and nano zero-valent iron (nZVI) is a new material in the field of environmental remediation. Little is known about how BDE-209 and nZVI combined exposure influences soil organisms. During the 28 days study, we determined the effects of single and combined exposures to BDE-209 and nZVI on the oxidative stress and metabolic response of earthworms (Eisenia fetida). On day 7, compared to CK, malondialdehyde (MDA) content increased in most combined exposure groups. To remove MDA and reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were induced in most combined exposure groups. On day 28, compared to CK, the activities of SOD and CAT were inhibited, while POD activity was significantly induced, indicating that POD plays an important role in scavenging ROS. Combined exposure to BDE-209 and nZVI significantly affected amino acid biosynthesis and metabolism, purine metabolism, and aminoacyl-tRNA biosynthesis pathways, interfered with energy metabolism, and aggravated oxidative stress in earthworms. These findings provide a basis for assessing the ecological impacts of using nZVI to remediate soils contaminated with BDE-209 from electronic waste.

Insight into the differential toxicity of PFOA and PFBA based on a 3D-cultured MDA-MB-231 cell model.

Perfluoroalkyl substances (PFASs) are a category of high-concerned emerging contaminants which are suspected to correlate with various human adverse health outcomes including tumors. It is also a question whether short-chain PFASs are qualified alternatives under the regulation of long-chain PFASs. In this study, a three-dimensional (3D) culture system based on Gelatin methacrylate (GelMA) hydrogel matrix was used to investigate the impacts of 120-h perfluorooctanoic acid (PFOA) and perfluorobutanoic acid (PFBA) exposure of MDA-MB-231 cells. The results showed that PFOA exposure promoted the proliferation, migration, and invasion of MDA-MB-231 cells in an environmentally relevant concentration range (0.1 to 10 µM), exhibiting a clear malignant-promoting risk. In contrast, PFBA only showed a trend to induce non-invasive cell migration. Hippo/YAP signaling pathway was identified as the contributor to the differences between the two PFASs. PFOA but PFBA reduced YAP phosphorylation and increased the nuclear content of YAP, which further facilitated abundant key factors of epithelial-mesenchymal transition (EMT) process. Our results provided a new idea for the carcinogenicity of PFOA using a 3D-based paradigm. Although the effects by PFBA were much milder than PFOA in the current test duration, the cell model suitable for longer exposure is still necessary to better assess the safety of alternative short-chain PFASs.

Insight into Bioaccumulation of Decabromodiphenyl Ethane in Eisenia fetida Increased by Microplastics.

The rise of electronics inevitably induced the co-pollution of novel brominated flame retardants (NBFRs) and microplastics (MPs). However, studies on how they interact to influence their bioavailability are scarce. Here, we explored the influence mechanism of acrylonitrile butadiene styrene (ABS)-MPs on the bioaccumulation of decabromodiphenyl ethane (DBDPE) in soil-earthworm microcosms. The influence exhibited a temporal pattern characterized by short-term inhibition and long-term promotion. After 28 days of exposure, DBDPE bioaccumulation in a co-exposure (10 mg kg-1 DBDPE accompanied by 1000 mg kg-1 ABS-MPs) was 2.61 times higher than that in a separate exposure. The adsorption process in the soil, intestines, and mucus introduced DBDPE-carried MPs, which had a higher concentration of DBDPE than the surrounding soil and directly affected the bioavailability of DBDPE. MP-pre-exposure (100, 1000, and 10000 mg kg-1) reduced epidermal soundness, mucus secretion, and worm cast production. This eventually promoted the contact between earthworm and soil particles and enhanced the DBDPE of earthworm tissue by 6%-61% in the next DBDPE-postexposure period, confirming that MPs increased DBDPE bioaccumulation indirectly by impairing the earthworm health. This study indicates that MPs promoted DBDPE bioaccumulation via adsorption and self-toxicity, providing new insight into the combined risk of MPs and NBFRs.

Pathogens and antibiotic resistance genes during the landfill leachate treatment process: Occurrence, fate, and impact on groundwater.

Landfill leachate is an essential source of pathogens and antibiotic resistance genes (ARGs) in the environment. However, information on the removal behavior of pathogens and ARGs during the leachate treatment and the impact on surrounding groundwater is limited. In this study, we investigated the effects of leachate treatment on the removal of pathogens and ARGs with metagenomic sequencing, as well as the impact of landfill effluent on groundwater. It is shown that the leachate treatment could not completely remove pathogens and ARGs. Twenty-nine additional pathogens and twenty-nine ARGs were newly identified in the landfill effluent. The relative abundance of pathogens and multiple antibiotic resistance genes decreased after ultrafiltration but relative abundance increased after reverse osmosis. In addition, the relative abundances of Acinetobacter baumannii, Erwinia amylovora, Escherichia coli, Fusarium graminearum, Klebsiella pneumoniae, and Magnaporthe oryzae, as well as mdtH, VanZ, and blaOXA-53 increased significantly in the landfill effluent compared to the untreated leachate. The relative abundance of some mobile genetic elements (tniA, tniB, tnpA, istA, IS91) in leachate also increased after ultrafiltration and reverse osmosis. The size of pathogens, the size and properties of ARGs and mobile genetic elements, and the materials of ultrafiltration and reverse osmosis membranes may affect the removal effect of pathogens, ARGs and mobile genetic elements in leachate treatment process. Interestingly, the pathogens and ARGs in landfill effluent were transferred to groundwater according to SourceTracker. The ARGs, mobile genetic elements, and pathogens that are difficult to remove in the leachate treatment process, provide a reference for optimizing the leachate treatment process and improving the control of pathogens and ARGs. Furthermore, this study clarifies the effect of landfill leachate sources of pathogens and ARGs in groundwater.

Toxicity of ionic liquids against earthworms (Eisenia fetida).

Ionic liquids (ILs) are widely used in frontier fields because of their highly tunable properties. Although ILs may have adverse effects on organisms, few studies have focused on their effect on earthworm gene expression. Herein we investigated the toxicity mechanism of different ILs towards Eisenia fetida using transcriptomics. Earthworms were exposed to soil containing different concentrations and types of ILs, and behavior, weight, enzymatic activity and transcriptome were analyzed. Earthworms exhibited avoidance behavior towards ILs and growth was inhibited. ILs also affected antioxidant and detoxifying enzymatic activity. These effects were concentration and alkyl chain length-dependent. Analysis of intrasample expression levels and differences in transcriptome expression levels showed good parallelism within groups and large differences between groups. Based on functional classification analysis, we speculate that toxicity mainly occurs through translation and modification of proteins and intracellular transport functions, which affect protein-related binding functions and catalytic activity. KEGG pathway analysis revealed that ILs may damage the digestive system of earthworms, among other possible pathological effects. Transcriptome analysis reveals mechanisms that cannot be observed by conventional toxicity endpoints. This is useful to evaluate the potential environmental adverse effects of the industrial use of ILs.

Dynamics and mechanisms of bioaccumulation and elimination of nonylphenol in zebrafish.

Nonylphenol (NP) has been widely concerned for its endocrine disrupting effects. In this study, we investigated the accumulation and elimination of NP for the whole body and trunk of zebrafish (Danio rerio). The results show that the LC50 values of NP in zebrafish ranged from 474 µg·L-1 (24-h exposure) to 238 µg·L-1 (96-h exposure). Meanwhile, the NP concentrations in zebrafish during the depuration stage fitted the first-order kinetic model well, and the depuration rate constant (K2) was reduced from 0.412 d-1 to 0.2827 d-1 with higher NP. The half-life (t1/2) of NP was 1.75-2.45 d in the whole fish and 0.56-0.86 d in the trunk under low to high NP, respectively. Both the accumulation and elimination of NP in trunk were faster than those in whole fish, indicating the preferential transfer from viscera to muscle and rapidly diffusion in reverse. The bioconcentration factors (BCFSS) of NP were 104-112 L·kg-1 in whole body and 76-104 L·kg-1 in trunk, respectively, suggesting that the muscle was a major position for NP storage.

Brominated flame retardants (BFRs) in sediment from a typical e-waste dismantling region in Southern China: Occurrence, spatial distribution, composition profiles, and ecological risks.

Our study evaluated the current occurrence, composition, and spatial distribution of eight congeners of polybrominated diphenyl ethers (PBDEs) and seven novel brominated flame retardants (NBFRs) in sediment from Guiyu, a typical e-waste dismantling region in China. PBDEs levels ranged from 0.345 to 401,000 ng/g dw and NBFRs levels ranged from 0.581 to 73,100 ng/g dw. Almost all sediment samples contained high levels of BDE-209 and DBDPE, and the ratio of DBDPE/BDE-209 in sediments ranged from 0.0814 to 2.80 (mean: 0.879). The concentration and composition profiles for BFRs in sediments from both mainstream and tributaries of two major rivers in Guiyu reach (and adjacent downstream locations) differed significantly from those far from Guiyu town. Whereas the high presence of BFRs in Guiyu reflected the historical crude e-waste dismantling activities in the region; the locations far from Guiyu town were likely to receive BFRs from atmospheric deposition, not originated from the region, as BFRs in water-sediment are known to be able to migrate a limited distance along the river. Ecological risk assessment revealed that the low brominated congeners of PBDEs and BDE-209 posed an unacceptable risk to the sedimentary life at multiple locations. Our results updated our knowledge of BFRs contamination in Guiyu, suggesting the necessity of continuous source monitoring, control procedures, and sediment cleanup for BFRs.

Bioaccessibility evaluation of pharmaceuticals in market fish with in vitro simulated digestion.

The consumption of pharmaceuticals-contaminated aquatic products could pose risks to human health, and risk assessments considering bioaccessibility can provide better dietary recommendations. In this study, the bioaccessibility of 6 pharmaceuticals (sulfadiazine (SD), sulfapyridine (SPD), roxithromycin (ROX), tylosin (TYL), diclofenac (DIC) and carbamazepine (CMZP)) in several fish species collected from Shanghai markets was evaluated using in vitro simulated digestion. The total mixed pharmaceuticals concentration in freshwater fish were lower than those in marine fish, and statistics showed that the total concentrations of SD, SPD and CMZP in freshwater fish were significantly lower than those of marine fish (p < 0.05). The bioaccessible concentration of each pharmaceutical accounted for 26.3% (TYL) to 101.5% (CMZP) of the total concentration in market fish (n = 70). The bioaccessibility of 6 pharmaceuticals in species of fish was 18.8% (cutlassfish) to 99.6% (bream), which may be related to the physical-chemical properties of the pharmaceutical and the characteristics of the matrix (e.g. lipid content). According to health risk assessments, the consumption of market fish in Shanghai posed no remarkable risk to human health (hazard quotient < 0.099). Ignoring the bioaccessibility of pharmaceuticals in aquatic products might overestimate the human health risks by dietary exposure.

Deciphering of antibiotic resistance genes (ARGs) and potential abiotic indicators for the emergence of ARGs in an interconnected lake-river-reservoir system.

This study aimed to decipher the patterns of antibiotic resistance genes (ARGs) and linkages of key abiotic indicators with ARGs in an interconnected lake-river-reservoir system. The results showed that seasonal variations in the relative abundance of ARGs and mobile gene elements (MGEs) were significant (KW, p < 0.05). ARGs representative of fecal pollution and natural environment were primarily distributed in the river and reservoir, respectively. The lake, river, and reservoir shared 54.5% of ARGs subtypes, most of which are multidrug resistance genes encoding for efflux pumps. Network results showed that ARGs conferring resistance to aminoglycoside frequently co-occurred with class 1 integrons and Limnohabitans. The resistance risks were low and associated with non-corresponding ARGs, and the highest resistance risk was caused by enrofloxacin in the Dianshan Lake. Fluorescence indices derived from two methods exhibited consistent positive correlations with abundance of individual genes (i.e. aada1 and aadA2-03) as well as total aminoglycoside resistance genes (Pearson, p < 0.05). Moreover, ARGs indicators of human and animal fecal pollution showed linkages with humic-like and fulvic-like indices (Pearson, p < 0.05). The results provide novel insights into the roles of abiotic factors on indicating dynamics of ARGs in aquatic environment impacted by anthropogenic activities.

[Spatial and Temporal Variation of Phytoplankton Community Structure and Its Influencing Factors in Shanghai River Channels].

In order to explore the spatial and temporal characteristics of the phytoplankton community structure and its influencing factors in Shanghai rivers, the water quality and phytoplankton community structure at 44 river channel sites in a central urban area, new town area, and rural area in Shanghai were investigated from September to October 2018 (autumn) and July to August 2019 (summer). The results showed that:① Chlorophyta was the dominant phyla during the autumn and summer, and was followed by Cyanobacteria and Bacillariophyta. Cyanobacteria dominated the phytoplankton community in terms of density. The number of species and density of phytoplankton were 24% and 2.77 times higher, respectively, than those during the summer and autumn. The dominance of Microcystis sp. was obvious during the autumn (Y=0.16), but there was no absolute dominant species during the summer. ② The difference in the number of phytoplankton species among the three regions was not significant, and the density of the total phytoplankton and cyanobacteria species showed a similar spatial pattern:rural area > new town area > central urban area. Additionally, no significant difference was observed in the total phytoplankton and Cyanobacteria density among the three regions during the autumn (P>0.05), whereas it was 1.82 and 1.93 times higher, respectively, in the rural area in comparison to the central urban area during the summer (P<0.05). Montecarlo test results revealed that the main factors affecting the phytoplankton community structure during the autumn were secchi disk transparency (SD), total phosphorus (TP), total nitrogen (TN), and turbidimetry (Turb), whereas these were TN, Turb, SD, and pH during the summer. ③ The results of a redundancy analysis (RDA) indicated that during the autumn, the phytoplankton in the rivers of the new town area were mainly affected by Turb, TN, and TP, while the rural rivers were mainly affected by SD. During the summer, the phytoplankton in the rivers of the new town and rural areas were mainly affected by TN and Turb. The influencing factors in the central urban area were complex.

[Influence of Antibiotics on the Denitrification Process of Antibiotic Resistant Denitrifying Bacteria and the Analysis of Microbial Community Structure].

The removal rate of some antibiotics in urban sewage by conventional treatment is low, which leads to an increase in antibiotic resistant bacteria in natural water environments. To reduce the ecological harm of antibiotics to the water in towns, a risk control technique for degradation of microantibiotics by the co-metabolism of antibiotic resistant denitrifying bacteria was proposed. Using sodium acetate as an electron donor and maintaining the concentration of ofloxacin (OFLX) at 1 µg·g-1, gradually increasing the dominant growth of antibiotic degradation bacteria, denitrifying bacteria (DnB1), trace antibiotics and sodium acetate, and denitrifying bacteria (DnB2) with the presence of sodium acetate and nitrogen elements were cultured. The degradation effect of antibiotics through denitrification and the effects of antibiotics on denitrification of resistant denitrifying bacteria and the changes to the microbial community were investigated. The results showed that DnB2 had a significant degradation effect on OFLX compared to DnB1. The degradation to OFLX by DnB1 and DnB2 was 0.31 µg·g-1 and 16.14 µg·g-1, respectively. Increased OFLX concentration inhibited DnB1 denitrification activity in the short term. The denitrification process of DnB2 was less affected by OFLX. At the same time, high-throughput sequencing using the Illumina MiSeq platform was used. Based on the operational taxonomic unit information formed by the clustering of sequencing results, the diversity of each sample was compared and analyzed. The research results show that the relative abundance and diversity of the microbial community of DnB1 are higher than those of DnB2.

Analyzing the adsorptive behavior of Amoxicillin on four Zr-MOFs nanoparticles: Functional groups dependence of adsorption performance and mechanisms.

In this study, four functional Zr-MOFs (UiO-66-H, -NH2, -NO2, -Cl) were prepared, characterized (FESEM, XRD, BET, XPS, FT-IR) and compared to remove low-concentration Amoxicillin (AMX) from water. Then UiO-66-NH2 was selected for further experiments due to its highest adsorption capacity (2.3 ± 0.4 mg g-1). The adsorption process followed pseudo-second order, Langmuir and Freundlich models. With pH increasing, deprotonation of functional groups in UiO-66-NH2 and AMX made adsorption interactions variable. The obvious spectra shift of FT-IR/XPS indicated that Lewis acid-base interaction was the main adsorption impetus; meanwhile hydrogen bonding interaction and π-π/n-π (electron-donator-acceptor) EDA interaction should be included. For Lewis acid-base interaction, the strength was controlled by percentage of amine group in UiO-66-NH2, mainly interacting with phenolic hydroxyl group in AMX. Due to changes in charge distribution of functional groups, there existed six kinds of π-π/n-π EDA interactions and thirteen types of hydrogen/π-hydrogen bonding interactions. Additionally, electrostatic interaction and molecular attraction also contributed to the AMX adsorption. Conclusively, analysis of functional groups interactions could help to comprehend adsorption mechanisms more profoundly and exploit functional adsorbents more efficiently.

The long-term environmental risks from the aging of organochlorine pesticide lindane.

Although increased contact time (aging) of pesticides in the soil decreases their bioavailability, this does not mean that the bound residues formed during the aging process pose fewer risk to the soil environment. Here the earthworm Eisenia fetida was exposed to organochlorine pesticide lindane in soil under different durations of lindane aging and exposure. The results of de novo RNA sequencing followed by molecular and biochemical validations demonstrated the aged lindane showed a different tendency to disrupt acetylcholine (ACh) transmission with the effects of fresh lindane to gamma-aminobutyric acid. Using own-developed earthworm activity test, we confirmed aged lindane prompted earthworms to exclusively exhibit a significant hypoactivity in locomotion, which could be explained by the inhibition of Ach system. This study suggested that the toxicity of pesticides would not depend solely on their free state components, and the awareness of long-term environmental risks from the bound states needs to be raised.

[Accumulation of Cd in Different Crops and Screening of Low-Cd Accumulation Cultivars].

Pot-culture experiments were carried out in Shanghai to screen crop varieties with low bioaccumulation properties with respect to cadmium (Cd). Eight common crops, such as green pepper, cucumber, cowpea, spinach, cauliflower, tomatoes, rice, and wheat, were planted in contaminated soil with different Cd concentrations of 0.23, 0.6, 1.2, 1.8, 2.4, and 3.0 mg·kg-1 to investigate the effects on biomass, Cd accumulation characteristics, and edible risk safety. The results indicated that:① With the increase in soil Cd content, the aboveground biomass of crops increased firstly and then decreased. The different crop types had different tolerance to Cd, with green pepper showed the strongest tolerance and spinach and tomato showed the least tolerance. ② The bioaccumulation factor of Cd in the edible parts of eight crops ranged in order of wheat > spinach > rice > green pepper > cauliflower > tomato > cucumber > cowpea. ③ Total Cd content in soil was significantly correlated with Cd content in the crops (P<0.05), and the order of the correlation coefficients was spinach > wheat > tomato > cucumber > green pepper > rice > cauliflower > cowpea. ④ The risk threshold value of Cd in soil based on the edible safety of different crops ranged in order of cowpea > cucumber > cauliflower > green pepper > tomato > rice > spinach > wheat. Cucumber, cowpea, and cauliflower were selected as the low-Cd-accumulating varieties according to their tolerance to soil Cd, bioaccumulation capacity, and edible risk threshold values.

State of the art of tertiary treatment technologies for controlling antibiotic resistance in wastewater treatment plants.

Antibiotic resistance genes (ARGs) have been considered as emerging contaminants of concern nowadays. There are no special technologies designed to directly remove ARGs in wastewater treatment plants (WWTPs). In order to reduce the risk of ARGs, it is vital to understand the efficiency of advanced treatment technologies in removing antibiotic resistance genes in WWTPs. This review highlights the application and efficiency of tertiary treatment technologies on the elimination of ARGs, s, based on an understanding of their occurrence and fate in WWTPs. These technologies include chemical-based processes such as chlorination, ozonation, ultraviolet, and advanced oxidation technology, as well as physical separation processes, biological processes such as constructed wetland and membrane bioreactor, and soil aquifer treatment. The merits, limitations and ameliorative measures of these processes are discussed, with the view to optimizing future treatment strategies and identifying new research directions.

DOM as an indicator of occurrence and risks of antibiotics in a city-river-reservoir system with multiple pollution sources.

Multiple sources contribute to the presence of antibiotic residues in water environments, and the environmental risks caused by antibiotics were paid more and more attention. This work aims to establish a relationship between optical properties of dissolved organic matter (DOM) and sources and risks of antibiotics. Occurrence of antibiotics and DOM in a city-river-reservoir freshwater system containing distinct antibiotic sources was investigated during three seasons using LC-MS and fluorescence excitation-emission matrix coupled with parallel factor analysis (EEM-PARAFAC), respectively. The results showed that antibiotics and DOM in the water had trends of increasing levels from the upstream to the midstream in the system. Five classes of antibiotics had statistically significant correlations with the humic-like component (C3) in the water (Pearson, p < 0.05). Especially, norfloxacin (NFX), which was dominant in the aquaculture source, significantly increased the fluorescence of C3 according to the fluorescence titration (R2 = 0.86, p < 0.01). Furthermore, fluorescence signature in the aquaculture pond posed broad humic acid-like peaks with relatively higher abundances compared to other areas. These results suggested that C3 could be recognized as an indicator of NFX from aquaculture sources. Meanwhile, C3 can largely account for ecological risks of tetracyclines according to the results of redundancy analysis. This work highlights the roles of EEM-PARAFAC on tracing the source of antibiotics and the correlations between environmental risks of antibiotics and DOM in the aquatic environment.

The toxic potentials and focus of disinfection byproducts based on the human embryonic kidney (HEK293) cell model.

Disinfection byproducts (DBPs) are inevitably generated during drinking water disinfection processes, and their hazards have not been well characterized. Because they plausibly cause toxicological and pathological damage to human kidney, we selected the human embryonic kidney (HEK293) cell, instead of the commonly used CHO cell, as a model to investigate the toxic potential and target of 10 DBPs, including 3 haloacetamides, 2 trihaloacetaldehydes and 5 iodomethanes. Based on the chronic toxicity parameter EC10 of the cell viability test, we obtained a toxic rank of the tested DBPs different from previous studies and calculated their risk quotients by combining their actual concentrations in drinking water systems. Then, dichloroacetamide (DCAM), trichloroacetaldehyde (TCAL), and bromochloroiodomethane (BCIM) were selected to conduct multiple mechanistic bioassays, including cellular lactate dehydrogenase (LDH) assay, ATP metabolism, ROS production, mitochondria-derived apoptosis and qRT-PCR assay. All bioassays revealed the effects of interrupting the molecular, physiological and biochemical processes relevant to mitochondrial functions, such as oxidative respiration, apoptosis, and energy metabolism. Our study improved the human risk assessment of DBPs with the help of a convenient model and parameter and revealed that mitochondrion is a potential toxic focus of DBPs exposure at the cellular level.

Chlorine migration mechanisms during torrefaction of fermentation residue from food waste.

Fermentation residue from food waster (FRFW) has a large amount of residual chlorine (Cl), and the high-salt of FRFW is either landfilled or treated as a fertilizer. The transfer of chlorine to the atmosphere and soil can cause pollution and soil salinization. This work primarily investigated the combined forms and migration mechanisms of Cl during the torrefaction of FRFW from 250 to 400 °C. The results showed that the form and amount of Cl released during the torrefaction of FRFW depended on temperature. The absolute content of soluble Cl and total Cl in torrefied solid products decreased, and the absolute content of insoluble Cl reached a maximum at 350 °C, which indicated that some soluble Cl was transferred to the insoluble Cl (CCl forms). The Cl-containing products in non-condensable gas was too little to be detected, so the majority of the reduced Cl was in liquids with different organic compounds.

Adsorption dynamics and mechanism of aqueous sulfachloropyridazine and analogues using the root powder of recyclable long-root Eichhornia crassipes.

In this study, we reclaimed the root powder of long-root Eichhornia crassipes (L.R.E.C.) as a biosorbent to remove aqueous sulfachloropyridazine (SCP) and other sulfonamides. The adsorption processes were investigated dependent on multiple measurements, including FT-IR and XPS analysis. The results confirmed that the basic amine group of neutral SCP molecules and the carboxyl hydroxyl on the surface of the root powder played the leading role in adsorption processes. Additionally, the experiments of ionic strength effect validated the involvement of electrostatic interaction in adsorption. Meanwhile, the adsorption data were fitted by various models and the results indicated that the Pseudo-second-order model and Freundlich model could well describe the adsorption processes, indicating the existence of physisorption and chemisorption as multi-layer adsorption. The maximum capacities of root powder for SCP were calculated to be 226.757 µg g-1 (288.15 K), 182.815 µg g-1 (303.15 K) and 163.132 µg g-1 (318.15 K) at pH of 3.0. The thermodynamic results revealed that the adsorption was a spontaneous and exothermic process. Moreover, the accordance with intra-particle diffusion presented that the adsorption processes could be divided into three steps and the reaction constant had a negatively linear relationship with the thickness of the boundary layer. The results proved that root powder of L.R.E.C. has great potential to remediate sulfonamides at practical level.

Adsorption and degradation of sulfadiazine and sulfamethoxazole in an agricultural soil system under an anaerobic condition: Kinetics and environmental risks.

Sulfonamides, one of the commonest antibiotics, were widely used on humans and livestock to control pathema and bacterial infections resulting in further environmental risks. The present study evaluated the adsorption and degradation of sulfadiazine (SDZ) and sulfamethoxazole (SMX) in an agricultural soil system under an anaerobic condition. Low sorption coefficients (Kd, 1.22 L kg-1 for SDZ and 1.23 L kg-1 for SMX) obtained from Freundlich isotherms experiment indicated that poor sorption of both antibiotics may pose a high risk to environment due to their high mobility and possibility of entering surface and ground water. Degradation occurred at a lower rate under the anaerobic environment, where both two antibiotics had higher persistence in sterile and non-sterile soils with degradation ratio <75% and DT50 > 20 d. Additionally, the addition of manure slightly increased degradation rates of SDZ and SMX, but there were no significant differences between single and repeated manure application at a later stage (p > 0.05), which suggested that the degradation was affected by both biotic and abiotic factors. Degradation rates would be slower at a higher concentration, indicating that degradation kinetics of SDZ and SMX were dependent on initial concentrations. During the degradation period, the antibiotics removal may change temperature, pH, sulfate and nitrate in soil, which suggested that the variation of antibiotics concentrations was related to the changes of soil physicochemical properties. An equation was proposed to elucidate the link between adsorption and degradation under different conditions, and to predict potential environmental risks of antibiotics.