Anisotropy in the Liquefaction Resistance of Fibre Reinforced Sand.

Adding discrete fibres to sand has been seen as a feasible technique to improve sand's strength as well as liquefaction resistance. Considering the anisotropic distribution of fibre orientations, the anisotropy in the liquefaction resistance of the reinforced sand is also introduced using fibres. Here, the triaxial compression and extension test results of unreinforced and fibre-reinforced sand in different density states are provided, from which the anisotropy in the liquefaction resistance of fibre-reinforced sand is demonstrated. Fibre reinforcement improves the liquefaction resistance of sand by introducing both the densifying effect and the confining effect. The inclusion of fibres increases both the slope and the intercept of the strength envelope in comparison with the unreinforced sand under triaxial compression, while the strength envelope is not affected by fibres under triaxial extension. Stress contribution of fibres makes the ESP of the composite under undrained loading reverse its direction to develop even though the phase transformation is absent. The stress ratio initiating the ESP reversal is irrespective of the fibre content but dependent on the density state under triaxial compression. Under triaxial extension, the stress ratio initiating the ESP reversal remains the same in the samples with varied density states and fibre contents. The mechanism correlating to the strength envelope and ESP reversal of the fibre-reinforced sand was demonstrated following a rule of mixture based constitutive modelling framework. By introducing an alternatively defined pore pressure ratio that incorporates the stress contribution of fibres, the liquefaction state of the fibre reinforced sand is reasonably assessed. Liquefaction remains absent in the sand once the fibres are mixed. The anisotropy in the liquefaction resistance of fibre-reinforced sand arises, as the predominant role played by the fibres to suppress the liquefaction is different when varied loading paths are involved, which is sourced from the anisotropic distribution of fibre orientations.

Bending and Crack Evolution Behaviors of Cemented Soil Reinforced with Surface Modified PVA Fiber.

To improve the flexural properties of cemented soils reinforced with fibers and avoid their brittle failure when subjected to complex loading conditions, a simple and cost-effective technique was explored to facilitate their application in retaining walls. In this study, how different fiber surface modifications, i.e., alkali treatment, acid treatment and silane coupling agent treatment, as well as different fiber contents, i.e., 0%, 0.25%, 0.5% and 1%, affect the bending properties of cemented soils was investigated by conducting three-point bending tests on notched beams. The digital image correlation (DIC) technology was used to examine the crack propagation process and the strain field distribution of cracks in specimens in the flexural tests. The results show that all fiber surface modifications increased peak strength and fracture energy, for example, the fracture energy of specimens AN1, AH1 and AK1 was increased by 180.4%, 121.5% and 155.4%, respectively, compared to PVA1. In addition, the crack tip strain, crack propagation rate and the initial crack width of the modified specimens were lower than those before modification. Lastly, scanning electron microscope (SEM) and mercury intrusion porosimetry tests were adopted to reveal the mechanism of bending performance in cemented soils reinforced by fiber surface modifications.

The Effects of Fiber Inclusion on the Evolution of Desiccation Cracking in Soil-Cement.

Desiccation cracking frequently occurs in mud, clay, and pavement. Understanding the evolution of desiccation cracking may facilitate the development of techniques to mitigate cracking and even prevent it from developing altogether. In this study, experimental investigations were performed focusing on the effects of fibers on the evolution of desiccation cracking in soil-cement. Varied types of fibers (i.e., jute fiber and polyvinyl alcohol fiber (PVA)) and fiber contents (i.e., 0%, 0.25%, 0.5%, and 1%) were involved. The digital image correlation (DIC) method was employed to capture the evolution and propagation of cracks in the soil-cement specimens when subjected to desiccation. The results show that the presence of fibers imposes significant effects on the crack propagation pattern as well as the area and length of the cracks in the soil-cement during shrinkage. The addition of fibers, however, insignificantly affects the evaporation rate of the specimens. The crack area and crack length of the specimens decreased significantly when more fibers were included. There were no macroscopic cracks observed in the specimens where the fiber content was 1%. The DIC method effectively helped to determine the evolution of displacement and strain field on the specimens' surface during the drying process. The DIC method is therefore useful for crack monitoring.

Analysis of the spleen proteome of chickens infected with reticuloendotheliosis virus.

Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the family Retroviridae, can result in immunosuppression and subsequent increased susceptibility to secondary infections. In the present study, we identified differentially expressed proteins in the spleens of chickens infected with the REV-A HLJ07I strain, using two-dimensional gel electrophoresis on samples from time points coinciding with different phases of the REV life cycle. Differentially expressed proteins were identified using one-dimensional liquid chromatography electrospray ionization tandem mass spectrometry (1D LC ESI MS/MS). Comparative analysis of multiple gels revealed that the majority of changes occurred at early stages of infection. In total, 60 protein spots representing 28 host proteins were detected as either quantitatively (false discovery rate [FDR] ≤0.05 and fold change ≥2) or qualitatively differentially expressed at least once during different sampling points. The differentially expressed proteins identified in this study included antioxidants, molecular chaperones, cellular metabolism, formation of the cytoskeleton, signal transduction, cell proliferation and cellar aging. The present findings provide a basis for further studies to elucidate the role of these proteins in REV-host interactions. This could lead to a better understanding of REV infection mechanisms that cause immune suppression.

Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens.

Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely elucidated. In this study, using multiplex branched DNA (bDNA) technology, we identified molecular mediators that participated in the regulation of the immune response during REV infection in chickens. Cytokine and chemokine mRNA expression levels were evaluated in the peripheral blood mononuclear cells (PBMCs). Expression levels of interleukin (IL)-4, IL-10, IL-13 and tumor necrosis factor (TNF)-α were significantly up-regulated while interferon (IFN)-α, IFN-ß, IFN-γ, IL-1ß, IL-2, IL-3, IL-15, IL-17F, IL-18 and colony-stimulating factor (CSF)-1 were markedly decreased in PBMCs at all stages of infection. Compared with controls, REV infected chickens showed greater expression levels of IL-8 in PBMCs 21 and 28 days post infection. In addition, REV regulates host immunity as a suppressor of T cell proliferative responses. The results in this study will help us to understand the host immune response to virus pathogens.

Detection of infectious laryngotracheitis virus by real-time PCR in naturally and experimentally infected chickens.

Infectious laryngotracheitis (ILT) is an acute, highly contagious upper-respiratory infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens experimentally infected with ILTV strain LJS09 and naturally infected chickens. The detection lower limit of the assay was 10 copies of DNA. There were no cross reactions with the DNA and RNA of infectious bursal disease virus, chicken anemia virus, reticuloendotheliosis virus, avian reovirus, Newcastle disease virus, and Marek's disease virus. The real-time PCR was reproducible as the coefficients of variation of reproducibility of the intra-assay and the inter-assay were less than 2%. The real-time PCR was used to detect the levels of the ILTV DNA in the tissues of specific pathogen free (SPF) chickens infected with ILTV at different times post infection. ILTV DNA was detected by real-time PCR in the heart, liver, spleen, lung, kidney, larynx, tongue, thymus, glandular stomach, duodenum, pancreatic gland, small intestine, large intestine, cecum, cecal tonsil, bursa of Fabricius, and brain of chickens in the infection group and the contact-exposure group. The sensitivity, specificity, and reproducibility of the ILTV real-time PCR assay revealed its suitability for detection and quantitation of ILTV in the samples from clinically and experimentally ILTV infected chickens.

Identification of a conserved B-cell epitope on reticuloendotheliosis virus envelope protein by screening a phage-displayed random peptide library.

BACKGROUND: The gp90 protein of avian reticuloendotheliosis-associated virus (REV-A) is an important envelope glycoprotein, which is responsible for inducing protective antibody immune responses in animals. B-cell epitopes on the gp90 protein of REV have not been well studied and reported. METHODS AND RESULTS: This study describes the identification of a linear B-cell epitope on the gp90 protein by screening a phage-displayed 12-mer random peptide library with the neutralizing monoclonal antibody (mAb) A9E8 directed against the gp90. The mAb A9E8 recognized phages displaying peptides with the consensus motif SVQYHPL. Amino acid sequence of the motif exactly matched (213)SVQYHPL(219) of the gp90. Further identification of the displayed B cell epitope was conducted using a set of truncated peptides expressed as GST fusion proteins and the Western blot results indicated that (213)SVQYHPL(219) was the minimal determinant of the linear B cell epitope recognized by the mAb A9E8. Moreover, an eight amino acid peptide SVQYHPLA was proven to be the minimal unit of the epitope with the maximal binding activity to mAb A9E8. The REV-A-positive chicken serum reacted with the minimal linear epitopes in Western blot, revealing the importance of the eight amino acids of the epitope in antibody-epitope binding activity. Furthermore, we found that the epitope is a common motif shared among REV-A and other members of REV group. CONCLUSIONS AND SIGNIFICANCE: We identified (213)SVQYHPL(219) as a gp90-specific linear B-cell epitope recognized by the neutralizing mAb A9E8. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against REV-A and other viruses of the REV group.

Development of a rapid and specific loop-mediated isothermal amplification detection method that targets Marek's disease virus meq gene.

A rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of Marek's disease virus (MDV) by amplification of conserved MDV meq gene sequences. LAMP is an innovative technique that allows the rapid detection of targeted nucleic acid sequences under isothermal conditions without the need for complex instrumentation. In this study, meq gene sequences were amplified successfully from different MDV strains by LAMP within 60min and no cross-reactivity was observed in a panel of related viruses that were associated with diseases of chickens. The detection limit of LAMP was 3.2 copies/million cells compared with 320 copies/million cells required for conventional PCR. Positive detection rates were assessed using either LAMP or PCR by examination of feather follicles that were collected from chickens infected experimentally with either strain J-1 (n=20) or strain Md5 (n=17), In addition to these samples, three isolates that were suspected to have been infected in the clinic were also tested. Results showed that the positive detection rate for LAMP was 95% (38/40), compared with 87.5% (35/40) and 90% (38/40) for strains J-1 and Md5 by PCR, respectively. These results indicated that the LAMP assay was more sensitive, rapid and specific than conventional PCR for the detection of MDV. This easy-to-perform technique will be useful for the detection of MDV and will aid in the establishment of disease control protocols.