Robinin inhibits pancreatic cancer cell proliferation, EMT and inflammation via regulating TLR2-PI3k-AKT signaling pathway.

PURPOSE: To investigate the anti-tumor effect of Robinin (Toll-like receptor 2 inhibitor) in pancreatic cancer cells via regulating tumor microenvironment. METHODS: The effects of Robinin on cell proliferation or migration in Mia-PACA2 and PANC-1 were determined, using CCK8 or wound healing assay, respectively. The typical markers of EMT (αSMA and snail) and the inflammation markers (IL-6 and TNF-α) were all detected by western blot. CU-T12-9 (TLR2 agonist) was used to rescue Robinin's effect. PI3k-p85α and Phosphorylated-AKT (p-AKT) were evaluated, compared to the ß-actin and AKT, using western blot. RESULTS: Robinin significantly inhibited cell proliferation and migration in Mia-PACA2 and PANC-1, compared to HPNE (**P < 0.01). Robinin also attenuated the expression of α-SMA and snail in Mia-PACA2, and PANC-1 (**P < 0.01). Besides, it was found that expression of IL-6 and TNF-α were diminished in presence of Robinin in Mia-PACA2, and PANC-1 (**P < 0.01). Western blot confirmed that Robinin could target on TLR2, and further downregulated PI3k-AKT signaling pathway to exert biological function. CONCLUSIONS: Robinin exerts anti-tumor effect perhaps via downregulating inflammation and EMT in pancreatic cancer cell through inhibiting TLR2-PI3k-AKT signaling pathway. Robinin may be a novel agent in adjuvant therapy of pancreatic cancer.

Identification of Fuzzy Rule-Based Models With Collaborative Fuzzy Clustering.

Fuzzy rule-based models (FRBMs) are sound constructs to describe complex systems. However, in reality, we may encounter situations, where the user or owner of a system only owns either the input or output data of that system (the other part could be owned by another user); and due to the consideration of data privacy, he/she could not obtain all the needed data to build the FRBMs. Since this type of situation has not been fully realized (noticed) and studied before, our objective is to come up with some strategy to address this challenge to meet the specific privacy consideration during the modeling process. In this study, the concept and algorithm of the collaborative fuzzy clustering (CFC) are applied to the identification of FRBMs, describing either multiple-input-single-output (MISO) or multiple-input-multiple-output (MIMO) systems. The collaboration between input and output spaces based on their structural information (conveyed in terms of the corresponding partition matrices) makes it possible to build FRBMs when input and output data could not be collected and used in unison. Surprisingly, on top of this primary pursuit, with the collaboration mechanism the input and output spaces of a system are endowed with an innovative way to comprehensively share, exchange, and utilize the structural information between each other, which results in their more relevant structures that guarantee better model performance compared with performance produced by some state-of-the-art modeling strategies. The effectiveness of the proposed approach is demonstrated by experiments on a series of synthetic and publicly available datasets.

Granular Fuzzy Rule-Based Modeling With Incomplete Data Representation.

Incomplete data are frequently encountered and bring difficulties when it comes to further processing. The concepts of granular computing (GrC) help deliver a higher level of abstraction to address this problem. Most of the existing data imputation and related modeling methods are of numeric nature and require prior numeric models to be provided. The underlying objective of this study is to introduce a novel and straightforward approach that uses information granules as a vehicle to effectively represent missing data and build granular fuzzy models directly from resulting hybrid granular and numeric data. The evaluation and optimization of this method are guided by the principle of justifiable granularity engaging the coverage and specificity criteria and carried out with the help of particle swarm optimization. We provide a collection of experimental studies using a synthetic dataset and several publicly available real-world datasets to demonstrate the feasibility and analyze the main features of this method.

Electrospun P(LLA-CL) Nanoscale Fibrinogen Patch vs Porcine Small Intestine Submucosa Graft Repair of Inguinal Hernia in Adults: A Randomized, Single-Blind, Controlled, Multicenter, Noninferiority Trial.

BACKGROUND: The aim of this study was to compare primary efficacy indicators of a low-cost, electrospun, nanoscale P(LLA-CL)/fibrinogen patch with a porcine small intestine submucosa patch for hernia repair. STUDY DESIGN: A randomized, single-blind, controlled multicenter trial was performed in 3 hospitals in Shanghai. Eligible patients (20 to 75 years old) with primary unilateral, reducible groin hernias were randomly assigned (1:1) to electrospun nanoscale P(LLA-CL)/fibrinogen patch (experimental group) or porcine small intestine submucosa (control group) patch groups. Patients were treated with the Lichtenstein technique, and the primary endpoint was hernia recurrence at 33 months after surgery. The secondary endpoints were postoperative complications including groin pain and operative site infections. RESULTS: Between July 2014 and February 2016, 172 patients were assigned to experimental (n = 86) and control (n = 86) groups. At 6-month follow-up, postoperative complications occurred in 5 patients (5 of 86, 5.81%) and 2 (2 of 86, 2.35%) patients in the control and experimental groups, respectively (p < 0.05). At 33-month follow-up, recurrence was observed in 2 patients (2 of 79, 2.53%) in the control group vs none in the experimental group (0 of 78) (the 95% CI difference between the experimental and control groups was -0.93% to 6.00% and within the preset noninferior margin of Δ10%). No significant differences were found in the degree of chronic pain and complications 33 months after surgery between the 2 groups. CONCLUSIONS: Because the recurrence rates and postoperative complications after 33 months were not inferior in the experimental group, we believe that the P(LLA-CL)/fibrinogen patch, as a low cost alternative, has prospects for widespread clinical use.

Swing mesh versus Modified Kugel mesh for primary inguinal hernia repair. A prospective randomized clinical trial

ABSTRACT PURPOSE To evaluate the safety and efficacy of a new mesh in the pre-peritoneal repair of inguinal hernia. METHODS We randomly divided 120 patients undergoing pre-peritoneal repair into 2 groups between March 2012 and December 2013. The patients were randomized to receive the Swing mesh (n=60; study group) or the Modified Kugel mesh (n=60; control group). The primary end point of this study was to compare postoperative groin pain of the two groups. Complications, recurrence and analgesic use were also recorded. RESULTS There were no recurrent cases in either group throughout the study period. There was no significant difference between the groups with respect to postoperative complications. The VAS of early postoperative pain was 1.32±1.69 in study group and 1.52±1.93 in control group, with the difference being not statistically significant (p = 0.547). Concerning chronic pain, no remarkable statistically significant difference was observed between the two groups at 3-month, 6-month, 12- and 18-month follow-up period. CONCLUSION Swing mesh can be safely and effectively used in inguinal hernia repair with the same advantage compared to the Modified Kugel mesh.

Swing mesh versus Modified Kugel mesh for primary inguinal hernia repair. A prospective randomized clinical trial.

PURPOSE: To evaluate the safety and efficacy of a new mesh in the pre-peritoneal repair of inguinal hernia. METHODS: We randomly divided 120 patients undergoing pre-peritoneal repair into 2 groups between March 2012 and December 2013. The patients were randomized to receive the Swing mesh (n=60; study group) or the Modified Kugel mesh (n=60; control group). The primary end point of this study was to compare postoperative groin pain of the two groups. Complications, recurrence and analgesic use were also recorded. RESULTS: There were no recurrent cases in either group throughout the study period. There was no significant difference between the groups with respect to postoperative complications. The VAS of early postoperative pain was 1.32±1.69 in study group and 1.52±1.93 in control group, with the difference being not statistically significant (p = 0.547). Concerning chronic pain, no remarkable statistically significant difference was observed between the two groups at 3-month, 6-month, 12- and 18-month follow-up period. CONCLUSION: Swing mesh can be safely and effectively used in inguinal hernia repair with the same advantage compared to the Modified Kugel mesh.