Effects of sulfate ions on growth and lipid synthesis of Scenedesmus obliquus in synthetic wastewater with various carbon-to-nitrogen ratios altered by different ammonium and nitrate additions.

Producing biodiesel from microalgae is a promising strategy to upgrade energy structure. In this study, effects of sulfate (SO42-) on lipid synthesis of Scenedesmus obliquus (S. obliquus) cultivated in synthetic wastewater with different carbon to nitrogen (C/N) ratios regulated by ammonium (NH4+-N) and nitrate (NO3--N), separately, were investigated. The results shown that SO42- could dramatically increase cell growth preferring to NH4+-N supply. And SO42- addition could improve its carbon and nitrogen utilization potential for boosting lipid productivity leading α-linolenic acid (C18:3n3) to occupy a dominant component (38.96%) in NH4+-N group at a C/N ratio of 7.5. Additionally, SO42- could enhance the enrichment and expression of up-regulated genes annotated in key enzymes such as GK, GNPAT, CRLS, plc and DEGS involved in glycerolipid, glycerophospholipid and sphingolipid metabolic pathways, resulting in carbon metabolism enhancement and sulfatide accumulation. This study brings a comprehensive view towards nutritional regulation of lipid synthesis in microalgae.

Effects of crab disturbance on nitrogen migration and transformation in a coastal tidal flat wetland.

The influence of crab disturbances on nitrogen migration and the transformations of pore water and overlying water in a coastal tidal flat wetland were investigated at the lab scale, and the nitrogen exchange flux at the sediment-water interface was calculated. The results showed that crabs, combined with tidal effects, had significant effects on the microtopography of the studied crab box. In addition, there was no significant difference in the concentrations of NH4+-N, NO3--N, or TN between two points in the horizontal direction (P > 0.05), and there were significant differences in the concentrations of NH4+-N and TN in the vertical direction (P < 0.05); the NO3--N concentration difference was not obvious (P > 0.05). The NO3--N concentration in the surface pore water of the crab box had a downward trend with time. Furthermore, the NH4+-N and TN contents in the overlying water in the crab box were significantly higher than those of the control box, indicating that crab disturbances also had significant effects on the concentrations of NH4+-N, NO3--N, and TN in the overlying water. The existence of crab caves greatly promoted the nitrogen exchange flux at the sediment-water interface, and the mean exchange fluxes of NH4+-N, NO3--N and TN were 51.40 mmol (m2 day)-1, -13.44 mmol (m2 day)-1 and 39.74 mmol (m2 day)-1, respectively (much higher than those measured in the control box), implying that NH4+-N and TN were released from the sediment to the overlying water, while NO3--N was released from the overlying water to the sediment.

Parameter-efficient bioclogging model: calibration and comparison with laboratory data.

A parameter-efficient bioclogging model coupled with hydrodynamics was developed with a stepwise numerical calculation. Column lab tests were carried out to calibrate and verify the bioclogging model developed in this paper. The results showed that the experimental data fit well with the simulation data, which indicated that the developed model was reasonable. According to the sensitivity analysis of the parameters, the BOD (biochemical oxygen demand) loading rate and deposition coefficient are the key parameters for bioclogging. The results illustrate how the clogging is impacted by changing the BOD loading rate and can predict the biofilm accumulation within the substrate, the microbial saturation along the substrate profile over time, and the biofilter longevity based on the biomass growth. The model could dynamically describe the entire process of biological clogging and could quantitatively predict the amount of biofilm accumulated in the pores with the increasing operation time, which provides a basis for the prediction of biological clogging. Graphical abstract.

Influence of clogging and resting processes on flow patterns in vertical flow constructed wetlands.

Vertical flow constructed wetlands are widely used for removing pollutants from wastewater. Substrate clogging is an operational challenge of constructed wetlands, which can result in impeded water flow and finally a significant decline in the ability of the system to treat the wastewater. The entire clogging process in a vertical flow constructed wetland (VFCW) was quantitatively analyzed by measurements of hydraulic conductivity. Tracer tests and model simulations were carried out to investigate internal flow patterns during the clogging and resting processes. This analysis revealed that hydraulic conductivity gradually decreased with operation time. Further, the distribution time of the flow field was different under different degrees of clogging. Non-uniformity in water flow was primarily observed in the first 400min after adding the tracer (NaCl) in the early clogging stage, as opposed to the last 400min in the late clogging stage. Variation in water flow divergence was closely correlated with piston flow; the reaction efficiency was highest in the early stages of clogging. In the later stages, stronger flow mixing was observed. Resting operations can reduce the dispersion of internal flow and improve reaction efficiency. After resting for approximately 15days, tracer concentration fluctuations decreased and internal flow back-mixing was alleviated. A simulation further described the internal flow pattern and elaborated and validated the tracer experiment. The outcomes of this study will assist in understanding how internal flow behavior varies in response to the clogging process and reveal details of the internal clogging mechanism in VFCWs.

High-throughput sequencing analysis of bacterial community spatiotemporal distribution in response to clogging in vertical flow constructed wetlands.

The aim of this study was to characterize bacterial communities in vertical flow constructed wetlands (VFCWs) using Illumina high-throughput sequencing. The bacterial communities developed lower richness and diversity in response to clogging. Bacterial diversity did not overtly decrease with depth. A variety of bacterial phyla were found in VFCWs' bacterial communities, including Bacteroidetes, Actinobacteria and Acidobacteria, among which Proteobacteria was dominant. At the genus level, a spatiotemporal variation was illustrated in the diversity and structure of bacterial communities. Clustering analysis of bacterial composition in the operational taxonomic units (OTUs) at the phylum and genus levels had a consistent trend, namely, that bacterial communities were more similar at similar column depths.

Evapotranspiration versus oxygen intrusion: which is the main force in alleviating bioclogging of vertical-flow constructed wetlands during a resting operation?

Clogging is the most significant challenge limiting the application of constructed wetlands. Application of a forced resting period is a practical way to relieve clogging, particularly bioclogging. To reveal the alleviation mechanisms behind such a resting operation, evapotranspiration and oxygen flux were studied during a resting period in a laboratory vertical-flow constructed wetland model through physical simulation and numerical model analysis. In addition, the optimum theoretical resting duration was determined based on the time required for oxygen to completely fill the pores, i.e., formation of a sufficiently thick and completely dry layer. The results indicated that (1) evapotranspiration was not the key factor, but was a driving force in the alleviation of bioclogging; (2) the rate of oxygen diffusion into the pores was sufficient to oxidize and disperse the flocculant biofilm, which was essential to alleviate bioclogging. This study provides important insights into understanding how clogging/bioclogging can be alleviated in vertical-flow constructed wetlands. Graphical abstract Evapotranspiration versus oxygen intrusion in alleviating bioclogging in vertical flow constructed wetlands.

Migration and degradation of swine farm tetracyclines at the river catchment scale: Can the multi-pond system mitigate pollution risk to receiving rivers?

The study investigated the degradation behaviors of swine farm tetracyclines (TCs) at a catchment scale and explored whether multi-pond systems could be beneficial to the interception of TCs so as to reduce the pollution risk to receiving rivers. The occurrence and migration of 12 kinds of tetracycline antibiotics, including their degradation products, were studied in four swine farms of the Meijiang River basin in China. The migration paths of the TCs were examined through sampling and analyzing the soil and/or sediment at different points along the swine wastewater outlet, which included sewer, sewage pond, mixed-canal (stream and sewage), farmland (paddy and upland soil) and finally the river. TC concentrations of all collected samples were obtained by solid phase extraction followed by measurement with high-performance liquid chromatography tandem mass spectrometry. The results showed that sediment TC concentrations varied greatly in different swine farms, from mg·kg-1 to µg·kg-1 levels. TCs had different decay patterns along different migration paths, such that TCs decayed exponentially in paddy soil, while linearly in sewer and mixed canal. The concentrations of TCs and their degradation products decreased in the order: sewer sediment > sewage pond sediment > mixed-canal sediment > paddy soil > upland soil, indicating that TCs tend to be more easily intercepted and accumulated in water-sediment systems such as ponds. Therefore, the multi-pond system could be an effective way to prevent TCs from migrating into rivers. These results provided essential information for contamination control of antibiotics in aquatic environments.

Applying a resting operation to alleviate bioclogging in vertical flow constructed wetlands: an experimental lab evaluation.

The aim of this study is to evaluate the effects of and analyze the reasons for applying a resting operation to alleviate bioclogging in vertical flow constructed wetlands (VFCWs). In parallel, three groups of laboratory-scale VFCWs were continuously fed with prepared wastewater (BOD = 600 mg/L) at a relatively high hydraulic loading rate of 0.5 m(3)/m(2)·d until clogging. Parameters related to the clogging of the wetland substrate before and after resting were examined and measured. The results showed that the resting operation could effectively alleviate bioclogging because the hydraulic conductivity and effective porosity were improved after 3, 7 and 10 days of resting. In the upper 0-10 cm layer, the hydraulic conductivity increased 2.0, 2.6 and 3.5 times, respectively, for the three resting periods. The reduction of the extracellular polymeric substance (EPS), biofilm decay and the consequential change in the biofilm structure are the main reasons that the resting operation relieved clogging. In addition, the observed and theoretical resting times (approximately 7 days) agreed well. The results provide a theoretical basis and technical support for solving clogging problems.