ABSTRACT
Endophytic and rhizospheric microorganisms associated with six native plants adapted to heavy metal polluted soil from Punta Olímpica and Chahuapampa, located in Callejón de Huaylas mountains, were evaluated as potential candidates for technologies to clean polluted ecosystems. It was selected 14 bacteria and 9 fungi strains by their iron and/or aluminum siderophore production trait, where BEP17-Dm showed higher production. According to the 16S rDNA analysis, bacteria belong to Pseudomonas, Bacillus, and Achromobacter genera, whereas by ITS analysis fungi belong to Talaromyces, Hypoxylon, Tolypocladium, and Penicillium. All bacteria strains tolerated lead (2-8 mM) and eigth tolerated cadmium (1-6 mM); also all fungi tolerated lead (9-70 mM) and cadmium (3-10 mM). Two bacteria and six fungi solubilized cadmium carbonate, while eleven bacteria and two fungi solubilized tricalcium phosphate, where P. japonica BEP18-Dm and B. subtilis BRU16-Sr exhibited higher solubilization index. None strains solubilized lead carbonate. BEP18-Dm produced higher concentration of IAA (53.42 µgml-1); while six bacteria and all fungi strains produced a low concentration of auxins. Medicago sativa seedlings inoculated with BEP17-Dm, BEP18-Dm, or BRU16-Sr showed more surviving percentage under in vitro culture in presence of Cd, Pb (0.5-1.0 mM), or Al (2.5-5.0 mM). Finally, it is the first report of siderophore-producing microorganisms from polluted soil of Callejón de Huaylas highlands, interestedly they displayed metabolic properties useful to enhance phytoremediation and biotechnology application.
Subject(s)
Bacteria/metabolism , Fungi/metabolism , Metals, Heavy/metabolism , Plants/microbiology , Siderophores/genetics , Siderophores/metabolism , Soil Pollutants , Bacteria/classification , Bacteria/genetics , Bacteria/isolation & purification , Biodegradation, Environmental , Environmental Restoration and Remediation , Fungi/classification , Fungi/genetics , Fungi/isolation & purification , Metals, Heavy/analysis , Peru , Plant Roots/microbiology , Soil MicrobiologyABSTRACT
En este trabajo se dan a conocer los resultados del análisis de heces de la Lechucita Bigotona (Xenoglaux loweryi), especie endémica de Perú y en peligro de extinción según IUCN, que habita los bosques montanos húmedos de la cordillera Oriental de los Andes. Entre enero y setiembre de 2014, durante evaluaciones sobre la ecología de este búho en el área de bosque montano conocido como "La Jalca", en la comunidad de Yambrasbamba, Amazonas; se colectaron cuatro muestras de heces de diferentes individuos, en los meses de mayo, julio, agosto y setiembre correspondientemente. Estos fueron analizados, encontrándose fragmentos característicos del orden Coleoptera, Hymenoptera, Ortoptera y Lepidoptera. Confirmándose que parte de su dieta son pequeños insectos; al igual que otros pequeños estrígidos.
We present results of an analysis of faecal samples from the long whiskered owlet (Xenoglaux loweryi), a species endemic to Peru and considered Endangered by the IUCN. It inhabits humid montane forests on the eastern slopes of the Andean cordillera. We collected four faecal samples from different individuals during ecological monitoring. Monitoring was carried out between January and September 2014 in the area known locally as "La Jalca" in the Yambrasbamba community, Amazonas. Samples were collected in May, June, August and September, respectively. Analysis revealed fragments characteristic of the orders Coleoptera, Hymenoptera, Ortoptera and Lepidoptera. Results confirm that small insects form part of the diet of the long whiskered owlet, similar to other small Strigidos.
