ABSTRACT
In this paper, coenzyme Q10 (Ubiquinone, CoQ10) was used for the first time as a transducer to construct electrochemical biosensor for effectively detecting γ-L-glutamyl-L-cysteinyl-glycine (glutathione, GSH). CoQ10 modified electrode was fabricated by attaching its gel mixed with multi-walled carbon nanotubes (MWNTs)/ionic liquid (IL). In the optimum conditions, based on the increasing of reduction peak current of CoQ10 caused by GSH through voltammetric technology, it was found that the peak current of CoQ10 was linear with the concentration of GSH in the range from 4.0×10(-9) to 2.0×10(-7)mol L(-1) at the pH 7.00, and the limit of detection was 3.2×10(-10)mol L(-1) (S/N=3). The results revealed that this method could be used to determine GSH in actual blood samples with the superiority of excellent selectivity, high stability and sensitivity. The strategy explored here might provide a new pathway to design novel multi-function transducers for detecting GSH, which has unique characteristic and potential application in the fields of sensor and medical diagnosis.
Subject(s)
Biosensing Techniques , Electrochemical Techniques/instrumentation , Glutathione/analysis , Ubiquinone/chemistry , Humans , Limit of DetectionABSTRACT
The title compound, C(28)H(38)Br(2)N(2), is centrosymmetric with the mid-point of the central C-C bond of the butyl group located on an inversion center. The terminal benzene ring is approximately perpendicular to the central 1,4-diaza-butadiene mean plane [dihedral angle = 78.23â (3)°]. No hydrogen bonding or aromatic stacking is observed in the crystal structure.
ABSTRACT
Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.
