ABSTRACT
The 5' nontranslated region (5'NTR) and nonstructural region nucleotide sequences of nine enzootic Venezuelan equine encephalitis (VEE) virus strains were determined, thus completing the genomic RNA sequences of all prototype strains. The full-length genomes, representing VEE virus antigenic subtypes I-VI, range in size from 11.3 to 11.5 kilobases, with 48-53% overall G+C contents. Size disparities result from subtype-related differences in the number and length of direct repeats in the C-terminal nonstructural protein 3 (nsP3) domain coding sequence and the 3'NTR, while G+C content disparities are attributable to strain-specific variations in base composition at the wobble position of the polyprotein codons. Highly-conserved protein components and one nonconserved protein domain constitute the VEE virus replicase polyproteins. Approximately 80% of deduced nsP1 and nsP4 amino acid residues are invariant, compared to less than 20% of C-terminal nsP3 domain residues. In two enzootic strains, C-terminal nsP3 domain sequences degenerate into little more than repetitive serine-rich blocks. Nonstructural region sequence information drawn from a cross-section of VEE virus subtypes clarifies features of alphavirus conserved sequence elements and proteinase recognition signals. As well, whole-genome comparative analysis supports the reclassification of VEE subtype-variety IF and subtype II viruses.
Subject(s)
5' Untranslated Regions/genetics , Antigens, Viral/genetics , Encephalitis Virus, Venezuelan Equine/genetics , RNA, Viral/genetics , Amino Acid Sequence , Animals , Antigens, Viral/chemistry , Base Sequence , Conserved Sequence , Equidae , Genetic Variation , Genome, Viral , Horses , Molecular Sequence Data , Phylogeny , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid , Species Specificity , Viral Proteins/chemistry , Viral Proteins/geneticsABSTRACT
Foi realizada uma pesquisa entre as puerperas de duas maternidades de Sao Paulo, procurando-se determinar algumas implicacoes medicas e socio-economicas do tabagismo em familias de baixa renda. Concluiu-se que uma parcela significativa do orcamento familiar e destinada ao tabagismo (media de 9,8%), sendo superior as fatias orcamentarias destinadas aos gastos com transporte (media 5,8%) e com a compra de leite (media de 8,3%)