Rapid Determination of Three Organic Acids in Polygonum Vivipari Rhizoma via One Marker by HPLC-UV at Equal Absorption Wavelength and Effervescence-Assisted Matrix Solid-Phase Dispersion.

A rapid HPLC-UV method for the determination of three organic acids (neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid) in Polygoni Vivipari Rhizoma (PVR) by one marker was developed. The sample was prepared by effervescence-assisted matrix solid-phase dispersion (EA-MSPD). The separation of compounds was performed on a Poroshell column. The equal absorption wavelength was set as follows: 292 nm (0∼7 min) and 324 nm (7∼10 min). The analytical time including sample extraction and HPLC separation time was 12 min. The analytical method validation such as accuracy (recoveries 99.85%-106.29% and RSD < 2.9%), precision (RSD < 1.3%), reproducibility (RSD < 1.7%), and stability tests (RSD < 0.7% in 24 h) proved that the established HPLC method was suitable for determination of three organic acids in PVR. The contents of three analytes obtained by the external standard method with three markers and the equal absorption wavelength method with one marker were similar (RSD ≤ 2.0%). The developed method, which is rapid and reference compound saving, is an improved quality evaluation method of PVR.

Development of a highly sensitive immunoassay for detecting aminopyrine abuse in herbal tea.

With the popularity of herbal tea in China, many food fraudsters have added illegal drugs to herbal tea to enhance its functions, among which aminopyrine is widely abused as an antipyretic and analgesic. Presently, there is no immunoassays for aminopyrine, and it is difficult to achieve real-time detection in the field. Based on a polyclonal antibody of aminopyrine with high specificity and sensitivity, an optimal combination of coating antigen/antibody was obtained by screening different coating antigens. On this basis, a sensitive ic-ELISA method was established to detect aminopyrine in herbal tea. The detection limit of the ic-ELISA was 0.18 ng mL-1, which was much lower than the 100 ng mL-1 required as a standard. The method had good consistency with LC-MS in the detection of actual samples and could be used as a reliable method for the detection of aminopyrine in herbal tea.

Worldwide Meniere's disease research: A bibliometric analysis of the published literature between 2002 and 2021.

Background: In recent years, there has been an increasing number of publications on Meniere's disease. However, there are no bibliometric research on Meniere's disease. The purpose of this study was to find the focus and trends of Meniere's disease research through bibliometric approach. Methods: Publications related to Meniere's disease in the Web of Science Core Collection (WOSCC) from 2002 to 2021 were collected. The bibliometric approach was used to estimate the searched data. Research foci of the studies were identified using VOSviewer and CiteSpace software. Results: A total of 1,987 articles meet the inclusion criteria and are included in the study. In the past 20 years, the number of Meniere's disease publications is gradually increasing, especially in the past 3 years. The country with the largest contribution to Meniere's disease research is the United States, followed by Europe and Japan. High-frequency keywords included Meniere's disease, endolymphaic hydrops, vertigo, meniere-disease, inner ear, dizziness, symptoms, hearing, diagnosis, and tentamicin. The analyses of keyword burst direction indicate that evoked myogenic potential, MRI, and committee are emerging research hotspots. Conclusion: This study provides an objective, systematic, and comprehensive analysis of Meniere's disease-related literature. In addition, we find a dramatic increase in studies in this field over the past 3 years. Evoked myogenic potentials and MRI may become the research hotspots of Meniere's disease in future. This study will help otolaryngologists, neurologists, and audiologists to clarify the research direction and potential hotspots of Meniere's disease and further help clinicians improve patients' prognosis.

Personalized nutrition: A review of genotype-based nutritional supplementation.

Nutritional disorders have become a major public health issue, requiring increased targeted approaches. Personalized nutrition adapted to individual needs has garnered dramatic attention as an effective way to improve nutritional balance and maintain health. With the rapidly evolving fields of genomics and nutrigenetics, accumulation of genetic variants has been indicated to alter the effects of nutritional supplementation, suggesting its indispensable role in the genotype-based personalized nutrition. Additionally, the metabolism of nutrients, such as lipids, especially omega-3 polyunsaturated fatty acids, glucose, vitamin A, folic acid, vitamin D, iron, and calcium could be effectively improved with related genetic variants. This review focuses on existing literatures linking critical genetic variants to the nutrient and the ways in which these variants influence the outcomes of certain nutritional supplementations. Although further studies are required in this direction, such evidence provides valuable insights for the guidance of appropriate interventions using genetic information, thus paving the way for the smooth transition of conventional generic approach to genotype-based personalized nutrition.

Characteristics and impact of postoperative surgical site infection on increased antibiotic duration among patients with laryngocarcinoma: a retrospective cohort study.

Background: Whether increased antibiotic duration is necessary for surgical site infection (SSI) in patients after neck surgery is unclear. We investigated the characteristics of SSI, and the impact of SSI on increased antibiotic duration among patients with laryngocarcinoma (LC). Methods: A retrospective cohort study including consecutive LC patients ≥18 years, undergoing surgery without remote metastasis was conducted from October 2015 to February 2022 in the Department of Otolaryngology-Head and Neck Surgery, Chengdu Second People's Hospital. SSI was defined according to current guidelines. Patients were stratified into 3 groups including no-infection, lower respiratory tract infection (LRTI) and SSI. Patient characteristics was recorded. Patients were followed up until discharge. A multiple linear regression model including SSI and other factors including age, sex, comorbidity and antibiotic treatments was performed to explore the impact of SSI on increased antibiotic duration among LC patients with postoperative infection. Results: A total of 88 patients were included, with 26 (29.5%) in no-infection group, 38 (43.2%) in LRTI group, and 24 (27.3%) in SSI group. Laryngocutaneous fistula occurred in 8 (33.3%) patients with SSI. Thirteen (34.2%) patients in LRTI group and 9 (37.5%) patients in SSI group experienced postoperative infection within 2 days after surgery, and antibiotic susceptibility tests were performed in 18 (47.4%) and 12 (50.0%) patients in LRTI and SSI group, respectively (P>0.05 for both). Levofloxacin and cefoperazone-sulbactam were the most commonly used antibiotics for postoperative infection in both LRTI and SSI groups (P>0.05 for both), irrespective of antibiotic susceptibility tests or not. The postoperative antibiotic duration in SSI group was significantly longer than that in LRTI group (13.62±4.28 days in SSI vs. 11.22±3.64 days in LRTI, P=0.021). A multiple linear regression analysis including SSI, age, sex, diabetes, antibiotic susceptibility test and hypoalbuminemia showed that, SSI was independently associated with increased antibiotic duration with LRTI as the reference among LC patients diagnosed [regression coefficient ß=3.02, 95% confidence interval (CI): 1.01-5.03, P=0.004], whereas antibiotic susceptibility test was not (P=0.467). Conclusions: SSI may be independently associated with increased postoperative antibiotic duration in patients with LC with or without antibiotic susceptibility test.

[A preliminary study of radionuclide imaging of the nasal lymphatic system in nasal polyps].

OBJECTIVE: To explore the role of nasal mucosal lymphatic drainage in the pathogenesis of nasal polyps. METHODS: There were 25 cases in the experimental group who had nasal polyps (which was further divided into Malm-1, Malm-2, Malm-3 level 3 subgroups) and 6 cases in the control group, including thyroid cancer and laryngeal cancer patients who had normal nasal structure. The nasal polyps in the experimental group and the middle turbinate in the control group were injected with a radionuclide and a radionuclide imaging technique was used to image the nasal mucosal lymphatics. The lymphatic drainage status of the nasal mucosa through the imaging results was analysed. RESULTS: The T/NT ratio (radioactivity counting) of the region of interest (ROI) was 20. 66 +/- 1.89 in the control group and 29. 33 +/- 6.34 in the experimental group. The difference was significant (t = 3.275, P < 0.05). The T/NT ratio of the ROI was 24.40 +/- 3.19 in the Malm-1 level group, 29.31 +/- 3.39 in the Malm-2 level group, 39.21 +/- 3.15 in the Malm-3 level group. The differences of qualitative analysis were significant (F = 38. 980, P < 0.05). The quantitative analysis showed that at the injection site, signs of lymphatic development and drainage were not found in the control group or experimental group, but the phenomenon of contrast media retention existed at the injection site in the experimental group. CONCLUSION: Lymphatic drainage dysfunction exists in patients with nasal polyps, and it may play a role in the pathogenesis of nasal polyps.

[Study on computed tomography features of nasal septum cellule and its clinical significance].

OBJECTIVE: To investigate the features of nasal septum cellule in computed tomographic (CT) images and its clinical significance. METHOD: CT scans data of nasal septum in 173 patients were randomly obtained from January 2001 to June 2005. Prevalence and clinical features were summarized in the data of 19 patients with nasal septum cellule retrospectively. RESULT: (1) Nineteen cases with nasal septum cellule were found in 173 patients. (2) All nasal septum cellule of 19 cases located in perpendicular plate of the ethmoid bone, in which 8 cases located in upper part of nasal septum and 11 located in middle. (3) There were totally seven patients with nasal diseases related to nasal septum cellule, in which 3 cases with inflammation, 2 cases with bone fracture, 1 case with cholesterol granuloma, 1 case with mucocele. CONCLUSION: Nasal septum cellule is an anatomic variation of nasal septum bone, and its features can provide further understanding of some diseases related to nasal septum cellule.