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1.
Eur Rev Med Pharmacol Sci ; 26(19): 7188-7194, 2022 10.
Article in English | MEDLINE | ID: mdl-36263528

ABSTRACT

OBJECTIVE: The objective of the present study was to examine the clinical factors influencing the pregnancy rate of infertile patients with endometriosis and establish a predictive model. PATIENTS AND METHODS: This study included 158 patients (158 cycles) with infertility and endometriosis who underwent laparoscopic surgery, and in vitro fertilization and embryo transfer (IVF-ET) were evaluated retrospectively between January 2019 and December 2020. The clinical factors in the pregnant and non-pregnant group were analyzed by univariate analysis. Statistically significant variables were subsequently used for multivariate logistic regression to establish the prediction model. RESULTS: Multivariate logistic regression analyses showed that GnRH-a treatment after operation (OR, 6.562; 95% CI: 2.782-15.477; p<0.01), ASRM stage (OR, 0.218; 95% CI: 0.093-0.509; p<0.05), the number of high-quality transferred embryos (OR, 3.155; 95% CI: 1.647-6.047; p<0.05) were independently associated with successful pregnancy. The area under the curve (AUC) of the prediction model was 0.774 (95% CI: 0.700-0.847). According to Hosmer-Lemeshow, the model was well fitted (p>0.05). We applied the bootstrapping method to internal validation, and the result showed that the pregnancy rate predicted by the model and the real data were consistent. CONCLUSIONS: The models for predicting pregnancy rates after IVF-ET in infertility and endometriosis patients showed high accuracy. The effective methods to increase the number of high-quality embryos need further study.


Subject(s)
Endometriosis , Infertility, Female , Pregnancy , Female , Humans , Pregnancy Rate , Endometriosis/complications , Retrospective Studies , Infertility, Female/surgery , Fertilization in Vitro , Gonadotropin-Releasing Hormone
2.
J Leukoc Biol ; 83(1): 173-80, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17895399

ABSTRACT

Dendritic cells (DCs), as specialized APCs, play a key role in the induction of anti-tumor immunity. They originate from bone marrow (BM) progenitors, which are frequently the targets of chromosomal translocations leading to development of leukemia. Aberrant DC differentiation and functions have been observed and are widely reported in patients with leukemia. It is not clear, however, whether such defects are a direct effect of a leukemic fusion gene or simply an outcome of the clinical disease. In this study, we demonstrate for the first time that knockin of the Mll-Een fusion gene can affect myeloid DC differentiation and functions directly, independent of the leukemic disease activities. We showed that the Mll-Een-expressing BM cells [enhanced green fluorescent protein+ (EGFP+)] from leukemic and nonleukemic mice had similarly impaired DC differentiation capacities with functional abnormalities. In contrast, BM cells without Mll-Een expression (EGFP(-)) showed normal DC differentiation and functions. A reduction in the frequency of CD11c+ DCs was also observed within the EGFP+ population in spleen and lymph nodes, and these cells were dysfunctional. Taken together, our findings suggest that the Mll-Een fusion gene can affect myeloid DC differentiation directly and functions in a cell-autonomous manner, where fully leukemic transformation of the hematopoietic progenitors is not required exclusively. Therefore, the study provides evidence for a direct causal relationship between leukemic gene fusion and abnormal DC differentiation, possibly contributing to the development of leukemia.


Subject(s)
Cell Transformation, Neoplastic/genetics , Dendritic Cells/immunology , Leukemia/genetics , Myeloid-Lymphoid Leukemia Protein/genetics , Animals , Cell Differentiation/genetics , Cell Differentiation/immunology , Cell Movement/immunology , Cell Transformation, Neoplastic/immunology , Cells, Cultured , Cytokines/biosynthesis , Flow Cytometry/methods , Leukemia/immunology , Mice , Mice, Inbred C57BL , Myeloid-Lymphoid Leukemia Protein/metabolism , T-Lymphocytes/immunology
3.
Acta Neurochir Suppl ; 95: 381-7, 2005.
Article in English | MEDLINE | ID: mdl-16463886

ABSTRACT

We evaluated the effects of defibrase DF-521 batroxobin on reducing brain edema formation and the expression of ICAM-1, complement C3d and C9 in the perihematomal area after intracerebral hemorrhage (ICH) in rats. A rat ICH model, involving infusion of autologous blood into the right basal ganglia, were used in this study. The animals were sacrificed at 24 and 72 hours after ICH to determine the water content of the brain tissue with wet/dry weight measurement. While the expression of ICAM-1 and complement C3d was detected using immuno-histochemistry, and C9 was detected semi-quantitatively with Western blot analysis in the perihematomal area. Perihematomal brain edema was reduced after intraperitoneally injection of DF-521 batroxobin 24 and 72 hours after intracerebral hemorrhage. Immunohistochemistry showed that there were less ICAM-I positive cells were found around the hematoma after intraperitoneally injection of DF-521 batroxobin 24 and 72 hours after ICH. Immuno-histochemistry also showed that C3d deposition reduced significantly, and the Western blot analysis also showed the content of C9 protein declined around the hematoma in DF-521 batroxobin treatment group at 72 hours after ICH. Defibrase DF-521 batroxobin down-regulate ICAM-1 and complement C3d and C9 expression in the perihematomal area, and attenuate brain edema formation in ICH rats.


Subject(s)
Batroxobin/administration & dosage , Brain Edema/drug therapy , Brain Edema/immunology , Cerebral Hemorrhage/drug therapy , Cerebral Hemorrhage/immunology , Complement Activation/immunology , Intercellular Adhesion Molecule-1/immunology , Animals , Brain Edema/etiology , Cerebral Hemorrhage/complications , Complement Activation/drug effects , Crotalid Venoms/administration & dosage , Male , Rats , Rats, Sprague-Dawley , Treatment Outcome
4.
J Immunol ; 167(9): 5338-47, 2001 Nov 01.
Article in English | MEDLINE | ID: mdl-11673550

ABSTRACT

Serum from patients with systemic lupus erythematosus (SLE) contained significantly higher concentrations of IL-18 than normal individuals. MRL/lpr mice, which develop spontaneous lupus-like autoimmune disease, also had higher serum levels of IL-18 than wild-type MRL/++ mice. Daily injections of IL-18 or IL-18 plus IL-12 resulted in accelerated proteinuria, glomerulonephritis, vasculitis, and raised levels of proinflammatory cytokines in MRL/lpr mice. IL-18-treated MRL/lpr mice also developed a "butterfly" facial rash resembling clinical SLE. In contrast, MRL/lpr mice treated with IL-18 plus IL-12 did not develop a facial rash. The facial lesion in the IL-18-treated mice showed epidermal thickening with intense chronic inflammation accompanied by increased apoptosis, Ig deposition, and early systemic Th2 response compared with control or IL-12 plus IL-18-treated mice. These data therefore show that IL-18 is an important mediator of lupus-like disease and may thus be a novel target for therapeutic intervention of spontaneous autoimmune diseases.


Subject(s)
Autoimmune Diseases/etiology , Interleukin-18/physiology , Adult , Animals , Antibodies, Antinuclear/blood , Autoimmune Diseases/pathology , Autoimmune Diseases/therapy , Cytokines/biosynthesis , Female , Humans , Interleukin-12/pharmacology , Lupus Erythematosus, Systemic/etiology , Mice , Mice, Inbred MRL lpr , Middle Aged
5.
J Cereb Blood Flow Metab ; 21(6): 722-33, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11488541

ABSTRACT

Cerebral ischemia resulting from a disruption of blood flow to the brain initiates a cascade of events that causes neuron death and leads to neurologic dysfunction. Reactive oxygen species are thought, at least in part, to mediate this disease process. The authors recently cloned and characterized an antioxidant protein, SAG (sensitive to apoptosis gene), that is redox inducible and protects cells from apoptosis induced by redox agents in a number of in vitro cell model systems. This study reports a neuroprotective role of SAG in ischemia/reperfusion-induced brain injury in an in vivo mouse model. SAG was expressed at a low level in brain tissue and was inducible after middle cerebral artery occlusion with peak expression at 6 to 12 hours. At the cellular level, SAG was mainly expressed in the cytoplasm of neurons and astrocytes, revealed by double immunofluorescence. An injection of recombinant adenoviral vector carrying human SAG into mouse brain produced an overexpression of SAG protein in the injected areas. Transduction of AdCMVSAG (wild-type), but not AdCMVmSAG (mutant), nor the AdCMVlacZ control, protected brain cells from ischemic brain injury, as evidenced by significant reduction of the infarct areas where SAG was highly expressed. The result suggests a rather specific protective role of SAG in the current in vivo model. Mechanistically, SAG overexpression decreased reactive oxygen species production and reduced the number of apoptotic cells in the ischemic areas. Thus, antioxidant SAG appears to protect against reactive oxygen species-induced brain damage in mice. Identification of SAG as a neuroprotective molecule could lead to potential stroke therapies.


Subject(s)
Antioxidants , Brain Diseases/etiology , Brain Diseases/prevention & control , Brain Ischemia/complications , Free Radical Scavengers/metabolism , RNA-Binding Proteins , Adenoviridae/genetics , Animals , Apoptosis , Astrocytes/chemistry , Brain/metabolism , Cerebral Infarction/metabolism , Cerebral Infarction/prevention & control , Free Radical Scavengers/analysis , Free Radical Scavengers/therapeutic use , Gene Expression , Genetic Therapy , Genetic Vectors , Glial Fibrillary Acidic Protein/analysis , Humans , In Situ Nick-End Labeling , Mice , Neurons/chemistry , Reactive Oxygen Species/metabolism , Recombinant Proteins , Superoxides/metabolism , Transfection , Ubiquitin-Protein Ligases
6.
Curr Mol Med ; 1(4): 457-68, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11899090

ABSTRACT

T cells, as they develop in the thymus come to express antigen receptors. The specificity of these receptors cannot be predicted and must include many with potential anti-self reactivity. Those that encounter self-antigens, in association with self-MHC (major histocompatibility complex), with high affinity are inactivated and do not leave the thymus. Not all self-antigens however are expressed in the thymus and thus many potentially self-reactive T cells enter the periphery. It poses therefore a fundamental immunological question: how peripheral self-tolerance is maintained in health? Dendritic cells (DC) play a central role in the activation of T cells, especially naïve T cells. Their importance in initiating immune responses against pathogens has been well established. However, DC represent complex populations of cells. Recent advances in our knowledge including molecular understanding of DC/T cell interactions have begun to reveal another important dimension of DC functions in the periphery, being not only initiators but also regulators of the immune system. This review summarises recent findings on the roles of DC in the regulation of immune responses and the maintenance of peripheral tolerance, in an attempt to explain how break down of this may lead to immunopathologies and autoimmunity. The concept of a regulatory DC and its possible role in the generation of T regulatory cells in health and in diseases are also discussed. Based on these, the need for a "continuing education" of the immune system throughout one's life, in which DC are again the "tutors", is postulated.


Subject(s)
Dendritic Cells/immunology , Self Tolerance , T-Lymphocytes/immunology , Animals , Cell Differentiation , Dendritic Cells/classification , Humans , Immunologic Memory , Models, Biological , Signal Transduction , T-Lymphocytes/cytology
7.
Methods Mol Med ; 64: 29-41, 2001.
Article in English | MEDLINE | ID: mdl-21374247

ABSTRACT

Dendritic cells (DC) are rare cells in peripheral tissues, and their isolation from tissues is fraught with problems. Thus, the proportion of DC within a tissue that is extracted is unknown, isolation procedures may select for subpopulations, and the isolation procedure itself may affect their properties. As part of their life history, DC migrate from peripheral tissues, via peripheral, afferent lymph to lymph nodes, even in the absence of exogenous antigenic stimulation. They are extracted within the node and very few, if any, appear in efferent lymph. These lymph DC (L-DC) represent a population that has matured in the periphery, that may have acquired antigen (Ag), and that may be engaged in active Ag transport to lymph nodes. As such they are a physiologically relevant DC population. In large animals such as sheep and cattle, L-DC can be isolated by direct cannulation of peripheral lymphatics, but yields are relatively low. In rodents, direct cannulation of some peripheral lymphatics is possible (1), but yields of cells are minuscule. To get around this problem we and others (1 -7), have utilized lymphadenectomy as a means of collecting pseudo-afferent lymph. When lymph nodes are removed, over a period of weeks, the afferent and efferent lymphatics join as part of the healing process, leaving cells in peripheral lymph free to enter central lymph. Central lymphat ics are relatively easy to cannulate, and cannulation can be maintained for considerable periods of time (see Note 1).

8.
J Exp Med ; 191(3): 435-44, 2000 Feb 07.
Article in English | MEDLINE | ID: mdl-10662789

ABSTRACT

This study identifies a dendritic cell (DC) subset that constitutively transports apoptotic intestinal epithelial cell remnants to T cell areas of mesenteric lymph nodes in vivo. Rat intestinal lymph contains two DC populations. Both populations have typical DC morphology, are major histocompatibility complex class II(hi), and express OX62, CD11c, and B7. CD4(+)/OX41(+) DCs are strong antigen-presenting cells (APCs). CD4(-)/OX41(-) DCs are weak APCs and contain cytoplasmic apoptotic DNA, epithelial cell-restricted cytokeratins, and nonspecific esterase (NSE)(+) inclusions, not seen in OX41(+) DCs. Identical patterns of NSE electrophoretic variants exist in CD4(-)/OX41(-) DCs, intestinal epithelial cells, and mesenteric node DCs but not in other DC populations, macrophages, or tissues. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL)-positive DCs and strongly NSE(+) DCs are present in intestinal lamina propria. Peyer's patches and mesenteric but not other lymph nodes contain many strongly NSE(+) DCs in interfollicular and T cell areas. Similar DCs are seen in the ileum and in T cell areas of mesenteric nodes in gnotobiotic rats. These results show that a distinct DC subset constitutively endocytoses and transports apoptotic cells to T cell areas and suggest a role for these DCs in inducing and maintaining peripheral self-tolerance.


Subject(s)
Dendritic Cells/physiology , Epithelial Cells/immunology , Intestines/cytology , Lymph Nodes/physiology , T-Lymphocytes/physiology , Animals , Antigen-Presenting Cells/immunology , Apoptosis/immunology , Cells, Cultured , DNA Fragmentation , Dendritic Cells/immunology , Immune Tolerance , Immunohistochemistry , In Situ Nick-End Labeling , Lymph Node Excision , Lymph Nodes/cytology , Mesentery , Microscopy, Confocal , Rats
10.
Lupus ; 8(1): 29-38, 1999.
Article in English | MEDLINE | ID: mdl-10025597

ABSTRACT

We have investigated the effects of interleukin-2 (IL-2) and transforming growth factor-beta (TGF-beta) gene therapy on the progress of autoimmune disease in MRL-lpr/lpr mice, a murine model of systemic lupus erythematosus (SLE). These mice have uncontrolled proliferation of T cells, an impaired response to T cell mitogen and produce autoantibodies against nuclear antigens, including DNA. Immune complexes formed by these autoantibodies are believed to cause glomerulonephritis and vasculitis in lupus mice and human SLE. Since there is an imbalance of cytokine production in both SLE patients and lupus mice, we examined the effects of cytokine gene therapy on the progression of autoimmune disease in MRL-lpr/lpr mice. The mice were treated orally with a non-pathogenic strain of Salmonella typhimurium bearing the aroA-aroD- mutations and carrying the murine genes encoding IL-2 and TGF-beta. The bacteria synthesise and slowly release the cytokines in vivo. Our results show that, contrary to expectation, TGF-beta gene therapy produced no improvement in pathology and generally had opposite effects to those of IL-2. IL-2 gene therapy restored the defective T cell proliferative response to mitogen and suppressed the autoantibody response, glomerulonephritis and growth of lymphoid tumours.


Subject(s)
Genetic Therapy , Interleukin-2/genetics , Lupus Erythematosus, Systemic/therapy , Salmonella typhimurium/genetics , Transforming Growth Factor beta/genetics , Animals , Antibodies, Antinuclear/biosynthesis , Female , Kidney/pathology , Lupus Erythematosus, Systemic/immunology , Lupus Erythematosus, Systemic/pathology , Lymphocyte Activation , Mice , Mice, Inbred MRL lpr , T-Lymphocytes/immunology
11.
Eur J Immunol ; 28(12): 4062-70, 1998 12.
Article in English | MEDLINE | ID: mdl-9862342

ABSTRACT

We have previously reported that mice lacking inducible nitric oxide synthase (NOS2) developed enhanced Th1 cell responses. We now investigated the mechanism by which NO modulates Th1 cells differentiation. Peritoneal macrophages from NOS2-deficient mice infected with Leishmania major in vivo or stimulated with IFN-gamma or lipopolysaccharide (LPS) in vitro produced significantly higher levels of IL-12 than those from heterozygous or wild-type mice. A macrophage cell line, J774, produced significant amounts of IL-12 following activation with LPS, or LPS plus IFN-gamma. This could be markedly enhanced by the NOS inhibitor L-NG monomethyl arginine (L-NMMA), but profoundly inhibited by the NO-generating compound S-nitroso-N-acetyl-penicillamine (SNAP). The effect of NO in this system is selective, since SNAP enhanced and L-NMMA decreased TNF-alpha synthesis by LPS-activated J774 cells. The differential effect of NO on IL-12 and TNF-alpha is at the transcriptional level and is activation dependent. Since IL-12 is a major inducer of Th1 cells which produce IFN-gamma that can activate macrophages to produce IL-12, our data demonstrate that NO can be an inhibitor of this feedback loop, preventing the excessive amplification of Th1 cells which are implicated in a range of immunopathologies.


Subject(s)
Interleukin-12/immunology , Macrophages, Peritoneal/immunology , Nitric Oxide Synthase/deficiency , Nitric Oxide/immunology , Th1 Cells/immunology , Animals , Cell Communication/immunology , Cell Differentiation/immunology , Gene Expression Regulation/immunology , Interleukin-12/biosynthesis , Mice , Mice, Knockout , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase/immunology , Nitric Oxide Synthase Type II , Th1 Cells/cytology
12.
Neurochem Res ; 23(7): 991-6, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9690742

ABSTRACT

The present study is to determine the effect of mild hypothermia (MHT) on the release of glutamate and glycine in rats subjected to middle cerebral artery occlusion and reperfusion. The relationship between amino acid efflux and brain infarct volume was compared in different periods during MHT. Reversible middle cerebral artery occlusion was performed in Sprague-Dawley rats using a suture model. The rats were divided into four groups including (1) MHT during ischemia (MHTi), (2) MHT during reperfusion (MHTr), (3) MHT during ischemia and reperfusion (MHTi + r), and (4) a normothermic group (NT). Extracellular concentrations of glutamate and glycine in the cortex and striatum were monitored using in vivo microdialysis and analyzed using high-performance liquid chromatography. Morphometric measurements for infarct volume were performed using 2,3,5-triphenyltetrazolium chloride staining. The increase of glutamate and glycine in the ischemic cortex of the MHTi and MHTi + r rats during ischemic and reperfusion periods was significantly less than that of the NT rats (p < 0.05). However, there was no statistical difference among these groups in the peak of glutamate and glycine release in the striatum. Infarct volume paralleled the release of glutamate and glycine. The protective effect of MHTi and MHTi + r in reducing ischemia and reperfusion brain injury may be due to the attenuation of both glutamate and glycine release during ischemia and reperfusion.


Subject(s)
Glutamic Acid/metabolism , Glycine/metabolism , Hypothermia/metabolism , Ischemic Attack, Transient/metabolism , Animals , Cerebral Cortex/metabolism , Chromatography, High Pressure Liquid , Corpus Striatum/metabolism , Male , Microdialysis , Rats , Rats, Sprague-Dawley
13.
J Immunol ; 160(9): 4143-7, 1998 May 01.
Article in English | MEDLINE | ID: mdl-9574511

ABSTRACT

MRL/MP-lpr/lpr (MRL/lpr) mice have a single mutation (lpr) of the fas apoptosis gene. The mutant mice developed significantly smaller lesions than the wild-type mice at the earlier stage of infection with the intracellular protozoan parasite Leishmania major. However, while all the wild-type mice achieved complete lesion resolution, the disease in the mutant mice progressed inexorably. The mutant mice had more IL-12 and nitrite/nitrate in the serum than wild-type mice following infection. Lymphoid cells from infected MRL/lpr mice produced more IFN-gamma but less IL-4 and IL-5 than cells from MRL-+/+ mice. Peritoneal macrophages from the mutant mice also produced more IL-12 and NO after stimulation with LPS. Thus, Fas expression is essential for resistance against leishmaniasis, and Fas-mediated apoptosis may form an integral part of the Th1-mediated microbicidal function.


Subject(s)
Genetic Predisposition to Disease , Leishmania major , Leishmaniasis, Cutaneous/genetics , Leishmaniasis, Cutaneous/immunology , Th1 Cells/immunology , fas Receptor/genetics , fas Receptor/immunology , Animals , Interleukin-12/biosynthesis , Interleukin-12/immunology , Mice , Mice, Mutant Strains , Mutation , Nitric Oxide/immunology
14.
J Gen Virol ; 79 ( Pt 4): 825-30, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9568978

ABSTRACT

Mice deficient in the inducible nitric-oxide synthase (iNOS), constructed by gene-targeting, were significantly more susceptible to herpes simplex virus (HSV)-1 infection, displayed a delayed clearance of virus from the dorsal root ganglia (DRG) and exhibited an increase in the frequency of virus reactivation in DRG compared with similarly infected heterozygous mice. The infected iNOS-deficient mice developed enhanced Th1-type immune responses and their spleen cells produced higher concentrations of IL-12 than similarly infected heterozygous mice. This finding suggests that iNOS plays an important role in resistance against HSV-1 infection. Furthermore, nitric oxide (NO) may block the development of Th1 cells via inhibition of IL-12 synthesis and thereby play a role in immune regulation.


Subject(s)
Herpes Simplex/etiology , Herpes Simplex/immunology , Herpesvirus 1, Human/pathogenicity , Nitric Oxide Synthase/deficiency , Th1 Cells/immunology , Animals , Female , Ganglia, Spinal/virology , Herpes Simplex/enzymology , Herpesvirus 1, Human/immunology , Herpesvirus 1, Human/isolation & purification , Heterozygote , Homozygote , Interleukin-12/biosynthesis , Male , Mice , Mice, Knockout , Nitric Oxide/immunology , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase/immunology , Nitric Oxide Synthase Type II
15.
J Exp Med ; 187(5): 787-94, 1998 Mar 02.
Article in English | MEDLINE | ID: mdl-9480988

ABSTRACT

T helper cell type 1 (Th1) and 2 (Th2) are central to immune regulation. However, no stable cell surface marker capable of distinguishing and separating these two subsets of CD4(+) cells has yet been found. Using differential display PCR, we have identified a gene encoding a cell membrane bound molecule, originally designated ST2L, T1, DER4, or Fit, expressed constitutively and stably on the surface of murine Th2s, but not Th1s even after stimulation with a range of immunological stimuli. Antibody against a peptide derived from ST2L strongly and stably labeled the surface of cloned Th2s but not Th1s, and Th2s but not Th1s derived from naive T cells of ovalbumin T cell receptor-alpha/beta transgenic mice. Three-color single cell flow cytometric analysis shows that cell surface ST2L coexpressed with intracellular interleukin (IL)-4, but not with interferon (IFN)-gamma. The antibody selectively lysed Th2s in vitro in a complement-dependent manner. In vivo, it enhanced Th1 responses by increasing IFN-gamma production and decreasing IL-4 and IL-5 synthesis. It induced resistance to Leishmania major infection in BALB/c mice and exacerbated collagen-induced arthritis in DBA/1 mice. Thus, ST2L is a stable marker distinguishing Th2s from Th1s and is also associated with Th2 functions. Hence, it may be a target for therapeutic intervention.


Subject(s)
Antigens, Differentiation, T-Lymphocyte/genetics , Th1 Cells/physiology , Th2 Cells/physiology , Animals , Arthritis, Experimental/immunology , Base Sequence , Female , Flow Cytometry , Gene Expression , Gene Expression Regulation, Developmental , Leishmaniasis/immunology , Male , Mice , Mice, Inbred BALB C , Mice, Inbred DBA , Molecular Sequence Data , RNA, Messenger/genetics
16.
Neurol Res ; 20(1): 57-62, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9471104

ABSTRACT

The aim of the present study is to compare the influence of timing and duration of mild hypothermia on rats subjected to 3 h of middle cerebral artery occlusion followed by 72 h of reperfusion. Sixty-four Sprague-Dawley rats were divided into three mild hypothermic groups according to the duration of mild hypothermia (MHT 32 +/- 0.2 degrees C): intra-ischemia (MHTi); intra-reperfusion (MHTr); and intra-ischemia/ reperfusion (MHTi + r). Our control group was normothermic (NT 37 +/- 0.2 degrees C). Reversible focal cerebral ischemia was carried out in rats with a suture technique. Cerebral blood flow was measured by laser Doppler flowmetry to confirm occlusion and reperfusion. The permeability of the blood-brain barrier was determined by the extravasation of Evans's blue dye, and infarct volumes were measured by 2,3,5-triphenyltetrazolium chloride staining at 72 h after reperfusion. Acute post-ischemic hyperperfusion and delayed hypoperfusion in the ischemic perifocal area and sustained hypoperfusion in the ischemic core were inhibited in MHTi + r and MHTi rats (p < 0.05) as compared to the NT rats. The action of MHTi + r on preventing post-ischemic progressive hypoperfusion in the perifocal area was more effective than that of MHTi 2 h after reperfusion (p < 0.05). Blood-brain barrier disruption in the basal ganglia and cortex areas was significantly reduced in both MHTi + r and MHTi groups, and especially the former. Infarct volume was significantly reduced in both MHTi and MHTi + r groups (p < 0.05). MHTi and MHTi + r have protective effects for reducing ischemia/reperfusion injury. The potential mechanisms may include inhibition of post-ischemic hyperperfusion, and delayed and sustained hypoperfusion.


Subject(s)
Blood-Brain Barrier , Evans Blue/pharmacokinetics , Hypothermia, Induced , Ischemic Attack, Transient/therapy , Reperfusion Injury/therapy , Animals , Arterial Occlusive Diseases/diagnostic imaging , Arterial Occlusive Diseases/therapy , Cerebrovascular Circulation , Disease Models, Animal , Ischemic Attack, Transient/diagnostic imaging , Laser-Doppler Flowmetry , Male , Rats , Rats, Sprague-Dawley , Reperfusion Injury/diagnostic imaging , Ultrasonography
17.
Transplantation ; 64(4): 589-93, 1997 Aug 27.
Article in English | MEDLINE | ID: mdl-9293870

ABSTRACT

BACKGROUND: During allograft rejection, up-regulation of cytokine-inducible nitric oxide synthase (iNOS) leads to the production of large amounts of nitric oxide (NO). The net effect of NO on the alloimmune response is, however, difficult to predict because of its diverse biological effects, which include potentially opposing roles as an effector and immunoregulatory molecule. METHODS: In this study, the role of iNOS on the in vitro and in vivo alloimmune response was defined using mutant mice that lack a functional iNOS gene. The ability of spleen cells obtained from iNOS-deficient mutants to proliferate and to produce cytokines in response to irradiated BALB/c stimulator cells was determined, and the rate at which iNOS-deficient mice were able to reject BALB/c skin allografts was observed. RESULTS: Spleen cells from homozygous iNOS-deficient (129xMF1)F1 mice, when compared with cells from heterozygous control mice, showed an increased in vitro proliferative response and produced substantially higher levels of interferon-gamma, and also more interleukin-2 and interleukin-12, in response to allogeneic stimulation. The kinetics of BALB/c skin graft rejection were comparable in heterozygous control animals and iNOS-deficient mice. Moreover, no net effect of iNOS on skin allograft rejection was apparent in mice treated with depleting monoclonal antibodies (mAb) to CD4 or CD8 T cells, either alone or in combination, or in mice treated with both anti-CD8 mAb and a neutralizing anti-tumor necrosis factor mAb. CONCLUSIONS: These results show that iNOS has an immunomodulatory effect on the in vitro alloimmune response but lack of iNOS has no net influence on the kinetics of murine skin allograft rejection in either unmodified recipients or recipients in which the early contribution of T-cell subsets and tumor necrosis factor-alpha to graft rejection has been abrogated.


Subject(s)
Nitric Oxide Synthase/metabolism , Skin Transplantation/immunology , Animals , Antibodies, Monoclonal/pharmacology , Female , Graft Rejection/enzymology , Graft Rejection/metabolism , Isoantigens/immunology , Kinetics , Lymphocyte Activation/immunology , Macrophage Activation/physiology , Mice , Mice, Inbred BALB C , Nitric Oxide Synthase/deficiency , T-Lymphocyte Subsets/immunology , Transplantation, Homologous/immunology , Tumor Necrosis Factor-alpha/immunology
19.
J Exp Med ; 184(4): 1519-24, 1996 Oct 01.
Article in English | MEDLINE | ID: mdl-8879223

ABSTRACT

We have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most (approximately 90%) were NSE- and CD68-, with only a minor population (approximately 10%) which were iNOS+, CD68+/NSE+. These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)-alpha. These results suggest that NO may mediate pathology in RA through the induction of TNF-alpha production.


Subject(s)
Arthritis, Rheumatoid/metabolism , Nitric Oxide/biosynthesis , Osteoarthritis/metabolism , Synovial Membrane/metabolism , Adult , Aged , Aged, 80 and over , Arthroplasty , Cells, Cultured , Fibroblasts/cytology , Fibroblasts/metabolism , Fluorescent Antibody Technique , Humans , Middle Aged , Synovial Membrane/cytology , Tumor Necrosis Factor-alpha/biosynthesis
20.
J Exp Med ; 183(4): 1447-59, 1996 Apr 01.
Article in English | MEDLINE | ID: mdl-8666903

ABSTRACT

MRL/MP-lpr/lpr (MRL/lpr) mice develop a spontaneous autoimmune disease. Serum from these mice contained significantly higher concentrations of nitrite/nitrate than serum from age-matched control MRL/MP-+/+ (MRL/+), BALB/c or CBA/6J mice. Spleen and peritoneal cells from MRL/lpr mice also produced significantly more nitric oxide (NO) than those from the control mice when cultured with interferon (IFN) gamma and lipopolysaccharide (LPS) in vitro. It is interesting to note that peritoneal cells from MRL/lpr mice also produced markedly higher concentrations of interleukin (IL) 12 than those from MRL/+ or BALB/c mice when cultured with same stimuli. It is striking that cells from MRL/lpr mice produced high concentrations of NO when cultured cells from MRL/+ or BALB/c mice. The enhanced NO synthesis induced by IFN-gamma/LPS was substantially inhibited by anti-IL-12 antibody. In addition, IL-12-induced NO production can also be markedly inhibited by anti-IFN-gamma antibody, but only weakly inhibited by anti-tumor necrosis factor alpha antibody. The effect of IL-12 on NO production was dependent on the presence of natural killer and possibly T cells. Serum from MRL/lpr mice contained significantly higher concentrations of IL-12 compared with those of MRL/+ or BALB/c control mice. Daily injection of recombinant IL-12 led to increased serum levels of IFN-gamma and NO metabolites, and accelerated glomerulonephritis in the young MRL/lpr mice (but not in the MRL/+ mice) compared with controls injected with phosphate-buffered saline alone. These data, together with previous finding that NO synthase inhibitors can ameliorate autoimmune disease in MRL/lpr mice, suggest that high capacity of such mice to produce IL-12 and their greater responsiveness to IL-12, leading to the production of high concentrations of NO, are important factors in this spontaneous model of autoimmune disease.


Subject(s)
Glomerulonephritis/etiology , Interleukin-12/metabolism , Lupus Erythematosus, Systemic/etiology , Nitric Oxide/metabolism , Animals , Disease Models, Animal , Dose-Response Relationship, Drug , Female , Interferon-gamma/metabolism , Interleukin-12/genetics , Interleukin-12/pharmacology , Kidney/pathology , Killer Cells, Natural/metabolism , Lipopolysaccharides/pharmacology , Mice , Mice, Inbred BALB C , Mice, Inbred CBA , Mice, Mutant Strains , Models, Biological , Peritoneum/cytology , Peritoneum/metabolism , Recombinant Proteins/pharmacology , Spleen/cytology , Spleen/metabolism , T-Lymphocytes/metabolism
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