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1.
Sensors (Basel) ; 22(16)2022 Aug 09.
Article in English | MEDLINE | ID: mdl-36015723

ABSTRACT

This paper presents a new method, a fast prediction method based on the Cartesian stiffness model and equivalent spring stiffness (FPM-CSES), to calculate displacement errors of deformation caused by low stiffness for industrial robot. First, the Cartesian stiffness model based on the Jacobian matrix was established for a robot, and then the displacement error model of deformations caused by external force was established based on Cartesian stiffness. Second, the transmission system of the robot's joint was analyzed, and an equivalent method for joint stiffness was presented based on a series spring system. Meanwhile, the stiffness of the key components including the servo motor, harmonic reducer, and timing belt was deduced in detail. Finally, a compared simulation and a measurement experiment were conducted on a 6-joint series robot. It was found that the FPM-CSES could calculate any configuration among the robot's workspace. Compared with the finite element analysis (FEA) method, the presented method is feasible and more efficient. The experimental results showed that the prediction accuracy of the FPM-CSES is rather high, with an average rate of more than 83.72%. Hence, the prediction method presented in this study is simple, fast, and reliable, and could be used to predict and analyze the displacement errors caused by the cutting force, and provide the basis for trajectory planning and error compensation, enhancing the robot's machining performance.

2.
Biochem Biophys Res Commun ; 301(2): 509-15, 2003 Feb 07.
Article in English | MEDLINE | ID: mdl-12565891

ABSTRACT

SRA is a steroid receptor co-activator which acts as a functional RNA and is classified as belonging to the growing family of functional non-coding RNAs. None of the different SRA transcripts described to date encode a detectable SRA protein following in vitro and in vivo translation experiments. We have identified three new SRA-RNA isoforms differing mainly from the originally cloned SRA by an extended 5(') extremity. These long SRA isoforms, able to encode a stable protein in vitro, led to the production in vivo of a nuclear protein when transfected into the MCF-7 human breast cancer cell line. Reverse-transcription polymerase chain reaction and Western blot analysis of RNA and protein extracts from different breast cancer cell lines confirmed the presence of endogenous coding SRA isoforms and their corresponding proteins. Our results demonstrate that full-length SRA-RNAs likely to encode stable proteins are widely expressed in breast cancer cell lines.


Subject(s)
RNA, Untranslated/genetics , RNA, Untranslated/metabolism , 5' Flanking Region , Amino Acid Sequence , Animals , Base Sequence , Breast Neoplasms , Female , Humans , Molecular Sequence Data , Open Reading Frames , Protein Isoforms/chemistry , Protein Isoforms/genetics , Protein Isoforms/metabolism , RNA/genetics , RNA, Long Noncoding , RNA, Untranslated/chemistry , Sequence Alignment , Tumor Cells, Cultured
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