ABSTRACT
Breast cancer is one of the most common malignant tumors among females, and can seriously affect the physical and mental health and even threaten the lives of women. Recently, research has demonstrated that microRNAs (miRNAs), as a new method of regulation, have been shown to have oncogenic and tumorsuppressive functions in human breast cancer. Detection of their expression may lead to the identification of novel markers for breast cancer. In the present study, we firstly detected miR340 expression and found lower expression of miR340 in 6 human breast cancer cell lines by using RTqPCR. Then by using wound healing assay and Transwell migration and invasion experiments, we focused on the role of miR-340 in the regulation of tumor cell migration and invasion, exploring the relationship between them. The results revealed that induction of miR340 expression was able to suppress tumor cell migration and invasion, whereas knockdown of miR340 expression promoted breast cancer cell migration and invasion. At the gene level, MYO10 (myosin X), as a direct miR340 target gene, mediated the cell migration and invasion. Finally, we verified our research further at the tissue specimen level and in animal experiments. In brief, miR340 plays an important role in breast cancer progression. Thus, miR340 may be further explored as a novel biomarker for breast cancer metastasis and prognosis, and potentially a therapeutic target.
Subject(s)
Breast Neoplasms/pathology , Gene Expression Regulation, Neoplastic/genetics , MicroRNAs/genetics , Myosins/metabolism , Animals , Blotting, Western , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Cell Line, Tumor , Cell Movement/genetics , Female , Gene Knockdown Techniques , Heterografts , Humans , Immunohistochemistry , Mice , Mice, Nude , Neoplasm Invasiveness/genetics , RNA, Small Interfering , Real-Time Polymerase Chain Reaction , TransfectionABSTRACT
OBJECTIVE: Microcapsule chemoembolism is a promising treatment of tumors. We describe a deep lingual arterial embolization of tongue carcinoma with microcapsuled carboplatinum. METHODS: Lingual artery cast specimens from cadavers were microscopically examined, and 78 patients with tongue cancer were recruited and treated with the deep lingual arterial embolization therapy. RESULTS: Microcapsule embolism occurred approximately at the fifth or sixth level of the deep lingual artery branches. The five-year survival rate was 88.5% (69 out of 78), and the ten-year survival rate 52.6% (41 out of 78). CONCLUSION: The deep lingual arterial embolization of tongue carcinoma with microcapsuled carboplatinum is an effective therapy to treat carcinoma in mid-margin or mid-body of the tongue.
Subject(s)
Antineoplastic Agents/administration & dosage , Carboplatin/administration & dosage , Chemoembolization, Therapeutic/methods , Drug Carriers/administration & dosage , Tongue Neoplasms/therapy , Capsules , Humans , Injections, Intra-Arterial , Tongue/drug effectsABSTRACT
OBJECTIVE: To evaluate the effect of applying the three dimensional craniomaxillofacial model technique in the restoration of mandibular defects with vascularized fibular autograft. METHODS: Three dimensional craniomaxillofacial models of 24 patients with mandibular defects were achieved by CT scan and rapid prototype technique. Surgery simulation and individual prefabricated titanium board were designed on the models. Vascularized fibular autograft was obtained, remodeled with titanium implant based upon preoperative surgery simulation, and implanted into the site with mandibular defect. RESULTS: By using three-dimensional craniomaxillofacial model, all fibular flaps survived without complication in all 24 patients. Bony union was achieved between the implanted fibula and the mandible. First intention of the wound was achieved. The appearance of the mandibular bone was well recovered shown by 3-dimensional CT scan. CONCLUSION: Based upon the preoperative surgery simulation with three dimensional craniomaxillofacial model, vascularized fibular autograft has many advantages for mandible reconstruction and represents the first choice for the head and neck surgeon.
Subject(s)
Bone Transplantation/methods , Fibula/transplantation , Imaging, Three-Dimensional , Mandible/surgery , Adolescent , Adult , Computer Simulation , Female , Humans , Male , Mandible/abnormalities , Middle Aged , Radiography , Skull/diagnostic imaging , Skull/surgeryABSTRACT
OBJECTIVE: To research the influence of palate bone implantation to the growth of maxilla in three dimensional direction. METHODS: 40 patients of congenital cleft palate type II with palate board damage were stochastically divide into the comparison group and bone planted group. In bone planted group, HA-Bone cement was used to repair palate tabular damage. All patients are 16 full year old, cephalometric radiograph and tooth cast model were measured and contrast analysis was applied. RESULTS: Planted bone group and the comparison group does not have the significance difference in both the maxilla length and maxilla hightness( P > 0.05); But there was significance difference in the width of post-part of the maxilla (P < 0.05). CONCLUSIONS: It could promote the growth of maxilla width in use of bone implantation, that advocated the bone repair in the sequence treatment process in cases of the hard palate bone damage.
