Ultrasonic coupled bioleaching pretreatment for enhancing sewage sludge dewatering: Simultaneously mitigating antibiotic resistant genes and changing microbial communities.

In this study, ultrasonic as a pretreatment coupled with bioleaching was used to enhance sludge dewaterability. Changes in microbial diversity and antibiotic resistant genes (ARGs) were studied during the combined treatment process. The results show that under optimal conditions, combined ultrasonic and bioleaching treatment led to decreases in the specific resistance of filtration and bioleaching time by 7.59% and 12.5%, respectively, compared with single bioleaching process. Using high pressure filtration system, the water content of sludge cake treated by the combined treatment was decreased to 58.04%, which was 10.04% lower than bioleaching sludge. After combined treatment, the microbial diversity and the total number of bacteria in the sludge decreased significantly, which caused the decreases in the absolute abundance of sulfonamide and tetracycline ARGs by 1.56-1.58 and 0.34-1.23 log units, respectively. However, the decrease in the total bacterial biomass was greater than the decrease in the number of potential hosts carrying the tetracycline ARG, resulting in an increase in the relative abundance of tetracycline gene. Furthermore, this study proposed a mechanism of the dewatering and ARGs, involving the combined ultrasonic and bioleaching treatment: Firstly, ultrasonic cavitation causes extracellular polymeric substances (EPS) to fall off the surface of sludge; Secondly, this faster and directly makes bacteria cells affected by bio-acidification and bio-oxidation. In this case, the cells could be more easily destroyed by the combined ultrasonic and bioleaching treatment, compared with individual bioleaching treatment; As a result, stronger dewaterability and more removal rates of ARGs were achieved under the combined treatment. The economic analyses showed that the combined ultrasonic and bioleaching treatment is a more practical and economical technique for achieving deep dewatering of sludge.

High-level waste activated sludge dewaterability using Fenton-like process based on pretreated zero valent scrap iron as an in-situ cycle iron donator.

A novel, recyclable, and rapid pre-ultrasound-thermal-acid-washed zero valent scrap iron/hydrogen peroxide (UTA-ZVSI/H2O2) method has been developed to effectively enhance waste activated sludge (WAS) dewaterability. The effects of UTA ultrasound densities, UTA temperature, newly generated iron solution, H2O2 concentrations, and WAS conditioning time on the WAS dewaterability were investigated using a bench-scale system. Results indicated that the UTA-ZVSI/H2O2 treatment significantly improved the WAS dewaterability. The water content of the dewatered cake decreased to 44.15 ± 0.98 wt% during optimal operational conditions, which was significantly lower than that achieved using Fenton-based processes. Based on this outcome, a three-step treatment mechanism involving UTA-ZVSI/H2O2 has been developed, including iron flocculation, hydroxyl radical oxidation, and skeleton building. The dewatering efficiencies of three types of representative WAS were consistently effective in the UTA-ZVSI/H2O2 reactor for up to 15 cycles. Efficiencies levels were significantly higher than those achieved with Fenton-based processes. Economic analysis illustrated that the developed UTA-ZVSI/H2O2 system was the most cost-effective among other WAS dewatering treatments. In addition, the treatment system significantly alleviated toxicity of heavy metals and phytotoxicity in the dewatered sludge. This supported subsequent agricultural use. In summary, this study provided a comprehensive and useful basis for improving WAS dewatering and subsequent disposal.

Mechanism of zero valent iron and anaerobic mesophilic digestion combined with hydrogen peroxide pretreatment to enhance sludge dewaterability: Relationship between soluble EPS and rheological behavior.

This study proposed a novel two-step conditioning strategy to enhance activated sludge (AS) dewatering performance. The method involved a zero valent iron (ZVI), anaerobic mesophilic digestion (AMD) process, and hydrogen peroxide (H2O2) oxidation. Response surface methodology (RSM) was applied to achieve optimum dewatering conditions. After the combined conditioning, dewatering was significantly better in the treated sludge compared to the raw AS. The specific resistance of filtration (SRF) of the treated sludge decreased to 2.48 × 1011 m/kg; this SRF level was 93.60% lower compared to the raw AS. The bound water content (BWC) decreased to 1.19 g/g dry solid (DS); this BWC level was 15.2% lower compared to the raw AS. The water content of the treated sludge cake decreased to 44.18 ± 0.46%. An economic analysis shows that ZVI-AMD-H2O2 can be used in real-world settings. Investigations of the underlying mechanisms showed that small block structures were formed after conditioning; viscosity and the colloidal forces of the sludge decreased; and organic matter and BWC were released from inner extracellular polymeric substances (EPS) layers to form soluble (SB)-EPS. This study illuminated the relationship between SB-EPS and the rheological behavior of AS. There is a high correlation coefficient between rheological parameter τy and N-containing substances in SB-EPS (R = -0.993, p < 0.05). The ZVI-AMD-H2O2 process effectively changed the EPS content, especially protein materials. This led to a decrease in AS viscosity and an increase in sludge dewaterability.

A new strategy on biomining of low grade base-metal sulfide tailings.

This study investigated the effect of designed microbial consortia on biomining of low grade base-metal sulfide tailings. The results show the amount of recycled metals were equal if the tailings were leached by mixed cultures of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans at three different ratios or by pure culture of L. ferriphilum, which was better than the pure culture of Acidithiobacillus ferrooxidans. qPCR (quantitative polymerase chain reaction) demonstrated only L. ferriphilum functioned in the mixtures at initial stage. The results of extracellular polymeric substances (EPS) via three-dimensional excitation-emission matrix combined with parallel factor analysis (3DEEM-PARAFAC) collected from mixed or pure cultures indicated there were no interactions between two strains. Secondary minerals were formed, but did not influence the leaching process. A new strategy for tailings biomining was proposed: only ferrous oxidizers should be added during the initial and middle biomining stage, while sulfur oxidizers should be added at the end.

Analysis of the relationship of extracellular polymeric substances to the dewaterability and rheological properties of sludge treated by acidification and anaerobic mesophilic digestion.

The initial pH value of sludge affects sludge anaerobic digestion and dewatering performance. In order to determine the suitable pH value and decrease the sludge digestion time, in this study, the effect and the mechanism of combined acidification and anaerobic mesophilic digestion (acid-AMD) on sludge dewaterability were investigated. The changes and relationships among the extracellular polymeric substances (EPS), physicochemical properties, and rheological behavior of the treated sludge were analyzed. The results indicated that the combined acid-AMD treatment improved sludge dewaterability approximately 36.08% and 30.28% compared to the acid conditioning and AMD treatment, respectively. The factors improving sludge dewaterability include a lower sludge pH value and appropriate duration of AMD, changes in particle size, surface properties and distribution of the EPS fractions. The acid-AMD treatment hydrolyzed the EPS, loosening the sludge structure. These changes reflected in the rheological properties of the sludge. After the treatment, the network strength and colloid force of the sludge weakened. The linear viscoelastic region contracted, and the sludge system became sensitive to shear. These results demonstrated that rheological analysis can help explain the sludge dewatering mechanism. The acid-AMD treatment effectively changed the distribution of EPS that play a vital role in sludge dewaterability.