ABSTRACT
MCM3AP-AS1 regulates the cartilage repair in osteoarthritis, but how it regulates osteogenic differentiation of dental pulp stem cells (DPSCs) remains to be determined. DPSCs were isolated and induced for osteogenic differentiation. MCM3AP-AS1 expression was increased along with the osteogenic differentiation of DPSCs, whose expression was positive correlated with those of OCN, alkaline phosphatase (ALP) and RUNX2. On contrary, miR-143-3p expression was decreased along with the osteogenic differentiation and was negatively correlated with those of OCN, ALP and RUNX2. Dual-luciferase reporter gene assay showed that miR-143-3p can be negatively regulated by MCM3AP-AS1 and can regulate IGFBP5. MCM3AP-AS1 overexpression increased the expression levels of osteogenesis-specific genes, ALP activity and mineralized nodules during DPSC osteogenic differentiation, while IGFBP5 knockdown or miR-143-3p overexpression counteracted the effect of MCM3AP-AS1 overexpression in DPSCs. Therefore, this study demonstrated the role of MCM3AP-AS1/miR-143-3p/IGFBP5 axis in regulating DPSC osteogenic differentiation.
Subject(s)
Acetyltransferases/physiology , Cell Differentiation/genetics , Dental Pulp/cytology , Gene Expression Regulation, Developmental/genetics , Gene Expression/genetics , Gene Expression/physiology , Insulin-Like Growth Factor Binding Protein 5/metabolism , Intracellular Signaling Peptides and Proteins/physiology , MicroRNAs/metabolism , Osteogenesis/genetics , RNA, Long Noncoding/genetics , RNA, Long Noncoding/physiology , Stem Cells/physiology , Acetyltransferases/genetics , Acetyltransferases/metabolism , Alkaline Phosphatase/metabolism , Cell Differentiation/physiology , Core Binding Factor Alpha 1 Subunit/metabolism , Gene Expression Regulation, Developmental/physiology , Humans , Intracellular Signaling Peptides and Proteins/genetics , Intracellular Signaling Peptides and Proteins/metabolism , Osteogenesis/physiology , RNA, Long Noncoding/metabolism , Stem Cells/metabolismABSTRACT
BACKGROUND: To investigate the correlation between abnormal glucose metabolism and insulin resistance in patients with liver cirrhosis. METHODS: A total of 254 participants were assigned into either the experimental group (EG) (n=123) or the normal group (NG) (n=131). We detected fasting blood glucose (FBG), postprandial blood glucose (PBG), fasting insulin (FINS), postprandial insulin, Hemoglobin A1c (HbA1c) and Insulin sensitivity index (ISI), at the same time, we compared various indexes in different Child-Pugh classification of the experimental group. RESULTS: The 1-hour PBG and 2-hour PBG in the EG were significantly higher than the NG group (P<0.05), serum insulin level in each period was significantly higher (P<0.05). The insulin sensitivity index (ISI) in the experimental group was statistical significantly lower (-4.21±0.09) VS. (-4.03±0.32), (P=0.031<0.05). Furthermore, the 2-hour PBG and FIN of Child-Pugh grade B patients were significantly higher than that of Child-Pugh grade A patients. The fasting insulin level of patients with cirrhosis of Child-Pugh grade C patients was significantly higher than that of Child-Pugh grade B patients, while FBG, PBG and ISI had no significant difference compared with those of Child-Pugh grade B patients. The higher the level of fasting blood glucose and postprandial blood glucose, the higher the FIN with the aggravation of liver function damage. CONCLUSIONS: Patients with liver cirrhosis had different degrees of insulin resistance. Clinicians can take proactive measures to prevent the occurrence of hepatogenic diabetes mellitus.
ABSTRACT
OBJECTIVE: To optimize the aqueous two-phase extraction and separation technology of flavonoids from the leaves of Eucommia ulmoides. METHODS: The system was prepared by dissolving suitable amounis of polyethylene glycol 4000 (PEG4000) and dextran 40000 ( D40) in aqueous solution. Phase map of the system, the mass fraction of PEG4000 and D40, the effects of amount of added sample, pH value and temperature on target compounds were investigated systematically. RESULTS: The optimum extraction conditions were PEG4000 11%, N40 8%, 8 g sample which contains 6.85% of flavonoids, temperature 60 t and pH value 8. The highest extraction rate could reach to 75.82%. CONCLUSION: This method has the advantages of mild conditions, solvent-free residues and less time-consumed, and the obvious efficiency in single-stage separation and purification of flavonoids.
