1.
Yi Chuan
; 27(3): 367-71, 2005 May.
Article
in Chinese
| MEDLINE
| ID: mdl-15985398
ABSTRACT
PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.