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Int J Biol Macromol ; 150: 1084-1092, 2020 May 01.
Article in English | MEDLINE | ID: mdl-31759003

ABSTRACT

A water-soluble heteropolysaccharide was isolated and purified from Enteromorpha prolifera by DEAE-52 and Bio-Gel P-2 column chromatography. Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), multi-angle laser light scattering (MALLS), and nuclear magnetic resonance (NMR) spectroscopy were used to characterize the structure of E. prolifera polysaccharide degradation (EPP-1). Its anti-oxidative activity was determined in Caenorhabditis elegans via modulation of microRNAs. The average molecular weight of EPP-1 was 4.28 kDa. It contained six types of linkage units as →2)-ß-d-GlcpA-(1→, →3,6)-ß-d-Manp-(1→, →4)-α-d-Glcp-(1→, →6)-ß-d-Galp-(1→, ß-l-Rhap-(1→, and →4)-ß-d-GalpA-(1→. The mean lifespan, ultraviolet-induced oxidative stress, and thermotolerance in C. elegans were improved after treatment of EPP-1. Moreover, EPP-1 significantly increased the total superoxide dismutase levels and decreased the malondialdehyde levels in C. elegans. Intracellular reactive oxygen species accumulation and DNA damage were ameliorated by up-regulation of SKN-1 and DAF-16 expression through miR-48 and miR-51 miR-186 down-regulation. In vivo studies demonstrated that EPP-1 might be applied in functional foods as the antioxidative and anti-ageing ingredient.


Subject(s)
Antioxidants , Caenorhabditis elegans/metabolism , Chlorophyta/chemistry , Gene Expression Regulation, Plant , MicroRNAs/biosynthesis , Oxidative Stress , Polysaccharides , RNA, Plant/biosynthesis , Ultraviolet Rays , Animals , Antioxidants/chemistry , Antioxidants/pharmacology , Caenorhabditis elegans/genetics , Gene Expression Regulation, Plant/drug effects , Gene Expression Regulation, Plant/radiation effects , MicroRNAs/genetics , Oxidative Stress/drug effects , Oxidative Stress/radiation effects , Polysaccharides/chemistry , Polysaccharides/pharmacology , RNA, Plant/genetics
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