ABSTRACT
To investigate the effects of the rare earth element cerium (Ce) on the life span and biomarkers of oxidative stress in the fruit fly (Drosophila melanogaster). Fruit flies were fed on media with different dose of ceric sulfate (1, 4, 16, 64, 256, 1024mg/L, corresponding to cerium concentrations of 0.45, 1.65, 6.91, 26.3, 104, and 429microg/g culture medium). Mean life span, maximum life span, and fertility were calculated. There was a significant decrease in mean life span and maximum life span with increasing doses of cerium. At some concentrations, there was a decrease in reproductive output, especially concentrations >6.91microg/g. We also measured superoxide dismutase (SOD) activity, catalase (CAT) activity, and lipid peroxidation product levels (malondialdehyde (MDA) content). Cerium caused a significant increase in MDA content and decrease in SOD and CAT activities at concentrations >6.91microg/g. These results suggest that cerium may result in oxidative toxicity to D. melanogaster.
Subject(s)
Cerium/toxicity , Drosophila melanogaster/drug effects , Longevity/drug effects , Oxidative Stress/drug effects , Animals , Catalase/metabolism , Dose-Response Relationship, Drug , Drosophila melanogaster/metabolism , Female , Fertility/drug effects , Male , Malondialdehyde/analysis , Superoxide Dismutase/metabolismABSTRACT
OBJECTIVE: The aim of this study was to determine the complete abortion rate for the vaginal administration of high-dose misoprostol after a failed medical abortion. MATERIALS AND METHODS: When their medical abortions failed after the conventional oral administration of mifepristone and misoprostol, participants then received 1,000 microg of misoprostol vaginally. The efficacy and side effects of this treatment were evaluated. RESULTS: Twenty-seven women who failed to abort after the conventional administration of mifepristone and misoprostol were enrolled in this trial. Fourteen days after the vaginal administration of 1,000 microg misoprostol, the overall complete expulsion rate had reached 88.8% (24/27). Most adverse effects were mild to moderate and did not require treatment. CONCLUSION: The vaginal administration of 1,000 microg misoprostol as a salvage therapy after a failed medical abortion appears to be a safe and highly effective alternative to surgical intervention.
Subject(s)
Abortifacient Agents, Nonsteroidal/administration & dosage , Abortion, Induced/methods , Misoprostol/administration & dosage , Adolescent , Adult , Female , Gestational Age , Humans , Misoprostol/adverse effects , Pregnancy , Time Factors , Treatment Outcome , Uterine HemorrhageABSTRACT
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza, sativa ssp. indica cv. Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity ofrDNA at individual loci may also vary through the cell cycle in rice. On extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers.
Subject(s)
DNA, Plant/genetics , DNA, Ribosomal/genetics , Oryza/cytology , Oryza/genetics , Cell Cycle/genetics , Chromosomes, Plant/genetics , In Situ Hybridization, FluorescenceABSTRACT
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also vary through the cell cycle in rice. on extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers
Subject(s)
DNA, Ribosomal/genetics , Cell Cycle/genetics , Chromosomes, Plant/genetics , DNA, Plant , In Situ Hybridization, Fluorescence , Oryza/cytology , Oryza/genetics , ChinaABSTRACT
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also vary through the cell cycle in rice. on extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers(AU)
Subject(s)
Cell Cycle/genetics , Chromosomes, Plant/genetics , DNA, Plant/genetics , DNA, Ribosomal/genetics , In Situ Hybridization, Fluorescence , Oryza/cytology , Oryza/genetics , ChinaABSTRACT
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also vary through the cell cycle in rice. on extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers(AU)
Subject(s)
Cell Cycle/genetics , Chromosomes, Plant/genetics , DNA, Plant/genetics , DNA, Ribosomal/genetics , In Situ Hybridization, Fluorescence , Oryza/cytology , Oryza/genetics , ChinaABSTRACT
Chromosomes are non-randomly distributed in interphase nucleus, and each chromosome occupies a distinct domain in the chromosome territories (CTs), the positioning and arrangement of CTs are well-organized, at the same time, the chromosome territories have definite dynamics characteristics, the strict positioning and spatial organization of chromosome territories are closely related with gene expression regulation. This paper reviewed progress in these respects.
Subject(s)
Chromosomes/genetics , Animals , Cell Nucleus/metabolism , Chromosome Positioning , Humans , Interphase/physiology , Models, GeneticABSTRACT
BACKGROUND AND PURPOSE: Reflux esophagitis of Los Angeles grade A or B is more common than grades C and D disease among Taiwanese. This study compared the efficacy of esomeprazole 40 mg and omeprazole 20 mg for starting on-demand therapy for grade A and B reflux esophagitis. METHODS: 100 patients with grade A and B reflux esophagitis were randomized to receive either esomeprazole 40 mg once daily (n = 50) or omeprazole 20 mg once daily (n = 50) for the first 4 weeks. Sustained symptomatic response (SSR) was defined as freedom from symptoms for the last 7 days of the 4-week treatment duration. On-demand therapy was used for the next 4 weeks in patients with SSR; patients without SSR continued with the same proton pump inhibitor regimen. Patients were asked to record their daily severity of acid regurgitation (AR) and heartburn (HB). Medication usage during on-demand therapy was recorded. RESULTS: Forty six patients in the esomeprazole group and 45 patients in the omeprazole group completed the study protocol. The rate of SSR was higher in the esomeprazole group than in the omeprazole group (per-protocol: 73.9% vs 51.1%, p < 0.05; intent-to-treat: 68% vs 46%, p < 0.05). The symptomatic scores for AR and HB were similar between patients taking medication continuously and those taking medication on-demand with both esomeprazole and omeprazole. For patients starting on-demand therapy, the total number of tablets used during 4 weeks was lower in the esomeprazole group than in the omeprazole group (13.5 vs 18.5, p < 0.05). CONCLUSIONS: In patients with grade A and B reflux esophagitis, esomeprazole 40 mg was more effective than omeprazole 20 mg for the initiation of on-demand therapy.
