Folate deficiency affects dendritic cell function and subsequent T helper cell differentiation.

Insufficient folate status may be related to the increasing prevalence of immune- or inflammation-related chronic diseases. To investigate the effects of folate on immune regulation, we examined the impact of folate deficiency (FD) on dendritic cell (DC) maturation and function and, thus, T helper (Th) cells differentiation. First, bone marrow-derived DCs (BMDCs) were generated from BALB/c mice bone marrow cells cultured in folate-containing (F-BMDCs) or folate-deficient (FD-BMDCs) medium. FD-BMDC displayed more immature phenotype including reduced levels of major histocompatibility complex class II (MHC II), co-stimulatory molecules and characteristic of higher endocytic activity. FD-BMDC produced less IL-12p70 and proinflammatory cytokines in response to lipopolysaccharide. This aberrant DC maturation due to FD resulted in reduced BMDC-induced Th cell activity and lower IL-2, IFNγ, IL-13 and IL-10 productions. Further in vivo study confirmed significantly lower IFNγ and IL-10 productions by T cells and showed higher splenic naïve Th and lower memory T, effector T and regulatory T cell (Treg) percentages in mice fed with the FD diet for 13 weeks. To investigate the role of DCs on T cell activity, splenic DCs (spDC) from FD mice were cocultured with Th cells. The FD spDC had lower MHC II and CD80 expressions and subsequently impaired DC-induced Th differentiation, shown as decreased cytokine productions. This study demonstrated that folate deficiency impaired DC functions and, thus, Th differentiation and responses, suggesting that folate plays a crucial role in maintaining Th cells homeostasis.

Large-area and thin light guide plates fabricated using UV-based imprinting.

As the demand of larger and thinner flat panel display increasing, conventional methods such as injection molding and hot embossing to fabricate light guide plates (LGPs) become difficult and unsuitable. This study reports a low-cost and high-throughput method to fabricate large-size (320 mm x 240 mm, 15" in diagonal) LGPs by using UV-based imprinting process. With the UV-based imprinting process, a large-size LGP with thickness down to 0.8 mm has been successfully fabricated. The optical property of fabricated LGP has been verified. This study has demonstrated the fabrication of large-size and thin LGPs by using UV-based imprinting process, and the possibility of UV-based imprinting process for fabricating other large thin optical elements.

Fabrication of large area resin microlens arrays using gas-assisted ultraviolet embossing.

Large-area microlens arrays are becoming important components in many applications such as LCD-TV diffusers. This paper reports a uniform pressure, low temperature process for their fabrication. The process integrates gas-assisted embossing and UV-curing embossing. During the process, the 230mm x 203mm PMMA substrate is pressed against the stainless-steel stamper coated with UV-curable resin. Under the gas pressuring and UV irradiating, a large array of microlens can be formed. By using this process, high embossing temperature and high embossing pressure can be avoided. Little residual stress is observed in the embossed PMMA substrate. The uniformity of large-area fabrication and optical properties of fabricated resin microlens array have been verified. This study has successfully shown the potential of this gas-assisted UV embossing process for the replication of large-area microstructures.

Fast fabrication of integrated surface-relief and particle-diffusing plastic diffuser by use of a hybrid extrusion roller embossing process.

Most plastic diffusers are either of surface-relief or particle-diffusing types, based on different principles and fabrication methods. This paper reports an innovative extrusion roller embossing process, which enables the fabrication of diffusers with both surface-relief and particle-diffusing functions. An extruder with die is employed to fabricate the thin film of PC/bead composite; the roller micro-embossing process is used to replicate the microstructure onto the surface of PC composite film. A metallic roller mold with microstructures is fabricated using turning process. During the extrusion rolling embossing process, the extruded film of PC with diffusion beads is immediately pressed against the surface of the roller mold. Under the proper processing parameters, the plastic diffusers integrating surface-relief and particle-diffusing functions have been successfully fabricated. The shape, uniformity, and optical properties of fabricated diffuser have been verified. This method shows the great potential for continuous fabrication of high-performance plastic diffusers integrating surface-relief and particle-diffusing functions with high throughput.

Fabrication of plastic microlens arrays using hybrid extrusion rolling embossing with a metallic cylinder mold fabricated using dry film resist.

This paper reports a novel and effective method to fabricate microlens arrays on polycarbonate films by hybrid extrusion rolling embossing. The metallic cylinder mold bearing an array of micro-holes is fabricated using photolithography with dry film resist. During the extrusion rolling embossing process, the extruded PC film is immediately pressed against the surface of the roller mold. Under the influence of the rolling pressure and surface tension, an array of convex microlenses is formed. The uniformity and optical properties have been verified. An efficient continuous mass production technique has been demonstrated.