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1.
Sci Rep ; 11(1): 1253, 2021 01 13.
Article in English | MEDLINE | ID: mdl-33441929

ABSTRACT

Melanoma is a skin cancer with great metastatic potential, which is responsible for the major deaths in skin cancer. Although the prognosis of melanoma patients has been improved with the comprehensive treatment, for patients with metastasis, the complexity and heterogeneity of diffuse diseases make prognosis prediction and systematic treatment difficult and ineffective. Therefore, we established a novel personalized immune-related gene pairs index (IRGPI) to predict the prognosis of patients with metastatic melanoma, which was conducive to provide new insights into clinical decision-making and prognostic monitoring for metastatic melanoma. Through complex analysis and filtering, we identified 24 immune-related gene pairs to build the model and obtained the optimal cut-off value from receiver operating characteristic curves, which divided the patients into high and low immune-risk groups. Meantime, the Kaplan-Meier analysis, Cox regression analysis and subgroup analysis showed that IRGPI had excellent prognostic value. Furthermore, IRGPI was shown that was closely associated with immune system in the subsequent tumor microenvironment analysis and gene set enrichment analysis. In addition, we broken through the data processing limitations of traditional researches in different platforms through the application of gene pairs, which would provide great credibility for our model. We believe that our research would provide a new perspective for clinical decision-making and prognostic monitoring in metastatic melanoma.


Subject(s)
Databases, Nucleic Acid , Gene Expression Regulation, Neoplastic/immunology , Melanoma/immunology , Melanoma/mortality , Models, Immunological , Disease-Free Survival , Female , Humans , Male , Melanoma/pathology , Neoplasm Metastasis , Survival Rate
2.
ChemistryOpen ; 9(2): 176-182, 2020 02.
Article in English | MEDLINE | ID: mdl-32025462

ABSTRACT

Fifteen novel furoxan-based nitric oxide (NO) releasing hybrids of estradiol derivatives were synthesized and evaluated in vitro anti-proliferative activity in MDA-MB-231, A2780, Hela and HUVEC cell lines. Most of them displayed potent anti-proliferative effects. Among the compounds, 4-bromo-3-((phenylsulfonyl)-1,2,5-oxadiazole 2-oxide)-oxy-propoxy-estradiol (11 b) exhibited the best activity with IC50 values of 3.58-0.0008 µM. Preliminary pharmacological studies showed that 11 b induced apoptosis and hardly affected the cell cycle of MDA-MB-231 cell line. NO-releasing capacity and inhibition of ERK/MAPK pathway signaling might explain the potent antineoplastic activity of these compounds. The preliminary structure-activity relationship (SAR) showed that steroidal scaffolds with a linker in 3-position were favorable moieties to evidently increase the bioactivities of these hybrids. Overall, these results implied that 11 b merited to be further investigated as a promising anti-cancer candidate.


Subject(s)
Estradiol/chemical synthesis , Oxadiazoles/chemical synthesis , Antineoplastic Agents , Apoptosis/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Drug Screening Assays, Antitumor , Estradiol/pharmacology , Humans , MAP Kinase Signaling System , Mitogen-Activated Protein Kinase Kinases/metabolism , Nitric Oxide/chemistry , Nitric Oxide/pharmacology , Oxadiazoles/pharmacology , Steroids/chemistry , Structure-Activity Relationship
3.
Nat Commun ; 9(1): 3783, 2018 09 17.
Article in English | MEDLINE | ID: mdl-30224715

ABSTRACT

Structural variations (SVs) exert important functional impacts on biological phenotypic diversity. Here we show a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic variations and improve the production of prodeoxyviolacein (PDV) by applying Synthetic Chromosome Recombination and Modification by LoxP-mediated Evolution (SCRaMbLE) in haploid yeast cells. The SCRaMbLE of ring_synV generates aneuploid yeast strains with increased PDV productivity, and we identify aneuploid chromosome I, III, VI, XII, XIII, and ring_synV. The neochromosome of SCRaMbLEd ring_synV generated more unbalanced forms of variations, including duplication, insertions, and balanced forms of translocations and inversions than its linear form. Furthermore, of the 29 novel SVs detected, 11 prompted the PDV biosynthesis; and the deletion of uncharacterized gene YER182W is related to the improvement of the PDV. Overall, the SCRaMbLEing ring_synV embraces the evolution of the genome by modifying the chromosome number, structure, and organization, identifying targets for phenotypic comprehension.


Subject(s)
Chromosomes, Artificial, Yeast , Genetic Engineering/methods , Saccharomyces cerevisiae/genetics , Aneuploidy , Gene Deletion , Genetic Variation , Genome, Fungal , Genotype , Haploidy , Indoles/metabolism , Microorganisms, Genetically-Modified , Phenotype , Polymerase Chain Reaction/methods , Saccharomyces cerevisiae/metabolism
4.
Steroids ; 101: 7-14, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26004429

ABSTRACT

Thirteen novel furoxan-based nitric oxide (NO) releasing hybrids (14a-e, 15a-e, 17b-d) of 16,17-pyrazo-annulated steroidal derivatives were synthesized and evaluated against the MDA-MB-231, HCC1806, SKOV-3, DU145, and HUVEC cell lines for their in vitro anti-proliferative activity. Most of the compounds displayed potent anti-proliferative effects. Among them, 17c exhibited the best activity with IC50 values of 20-1.4nM against four cell lines (MDA-MB-231, SKOV-3, DU145, and HUVEC), and 1.03µM against a tamoxifen resistant breast cancer cell line (HCC1806). Furthermore, five compounds (14a, 15a, 17b-d) were selected to screen for VEGF inhibitory activity. Compounds 15a, 17b,c showed obviously better activity than 2-Methoxyestradiol (2-ME) on reducing levels of VEGF secreted by MDA-MB-231 cell line. In a Capillary-like Tube Formation Assay, compounds 17b,c exhibited a significant suppression of the tubule formation in the concentration of 1.75nM and 58nM, respectively. The preliminary SAR showed that steroidal scaffolds with a linker in 3-position were favorable moieties to evidently increase the bioactivities of these hybrids. Overall, these results implied that 17c merited to be further investigated as a promising anti-cancer candidate.


Subject(s)
Androsterone/analogs & derivatives , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/pharmacology , Dehydroepiandrosterone/analogs & derivatives , Oxadiazoles/chemical synthesis , Oxadiazoles/pharmacology , Antineoplastic Agents/chemistry , Cell Line, Tumor , Cell Proliferation/drug effects , Chemistry Techniques, Synthetic , Humans , Oxadiazoles/chemistry , Vascular Endothelial Growth Factor A/antagonists & inhibitors
5.
J Med Chem ; 57(22): 9343-56, 2014 Nov 26.
Article in English | MEDLINE | ID: mdl-25350923

ABSTRACT

Sixteen furoxan-based nitric oxide (NO) releasing coumarin derivatives (6a-c, 8a-g, 10a, 13a,b, 15, and 17a,b) were designed, synthesized, and evaluated against the A549, HeLa, A2780, A2780/CDDP, and HUVEC cell lines. Most derivatives displayed potent antiproliferation activities. Among them, 8b exhibited the strongest antiproliferation activity on the four sensitive cell lines mentioned above and three drug resistant tumor cell lines A2780/CDDP, MDA-MB-231/Gem, and SKOV3/CDDP with IC50 values from 14 to 53 nM and from 62 to 140 nM, respectively. Furthermore, 8b inhibited the growth of A2780 in vivo and displayed lower toxicity on nontumorigenesis T29, showing good selectivity against malignant cells in vitro. Preliminary pharmacological studies showed that 8b induces apoptosis, arrests the cell cycle at the G2/M phase in the A2780 cell line, and disrupts the phosphorylation of MEK1 and ERK1. Overall, the NO-releasing capacity and the inhibition of ERK/MAPK pathway signaling may explain the potent antineoplastic activity of these compounds.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Coumarins/administration & dosage , Coumarins/chemistry , Drug Screening Assays, Antitumor , Neoplasms/drug therapy , Oxadiazoles/administration & dosage , Oxadiazoles/chemistry , Animals , Apoptosis , Carcinogenesis , Cell Cycle , Cell Line, Tumor , Cell Proliferation , Drug Combinations , Female , HeLa Cells , Human Umbilical Vein Endothelial Cells , Humans , Inhibitory Concentration 50 , MAP Kinase Kinase 1/metabolism , Mice , Mice, Inbred BALB C , Mice, Nude , Mitogen-Activated Protein Kinase 3/metabolism , Nitric Oxide/chemistry , Signal Transduction
6.
Steroids ; 78(14): 1339-46, 2013 Dec 20.
Article in English | MEDLINE | ID: mdl-24128809

ABSTRACT

This paper describes the regio- and stereoselective reduction of △4-3-keto moiety in certain steroids using Na2S2O4/NaHCO3 and CuCl/NaBH4, respectively. Using either one of the two reduction agents in the reaction, the 17-substituents in the D ring were observed to have clearly influenced the stereoselective reduction of 4-ene in the A ring by the so-called conformational transmission effect. Na2S2O4/NaHCO3 regioselectively reduced CC at 4-position of 17-substituted-androst-4-en-3-one derivatives to 5α-H-3-one as the main isomer. And as an extended application, Epiandrosterone (11) was further synthesized from androst-4-en-3,17-dione (AD) via four steps. The total yield from this was about 45%. In the presence of CuCl/NaBH4, △4-3-keto conjugated reduction of 17-spirocyclic ethylene ketal protected androst-4-en-3-one derivatives mainly produced 3α-hydroxy-5ß-H isomers, at a yield around 81%. Considering the scaffold configuration of 3α-hydroxy-5ß-H moiety coincided with that of bile acid analogs, this selective reduction could also be used as an alternative method for the synthetic study of bile acids using AD and its derivatives, which are from the microorganism degradation of natural sterols, as the potential materials. Meanwhile, configurations of the reductive compounds 5b, 6b, 9, 10 and 17e were identified by X-ray diffraction.


Subject(s)
Androstenedione/chemistry , Androsterone/chemical synthesis , Bile Acids and Salts/chemical synthesis , Borohydrides/chemistry , Copper/chemistry , Crystallography, X-Ray , Dithionite/chemistry , Sodium Bicarbonate/chemistry , Stereoisomerism
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