Potential diagnostic and prognostic value of plasma long noncoding RNA LINC00086 and miR-214 expression in gastric cancer.

BACKGROUND: Plasma carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and cancer antigen 72-4 (CA72-4) are common markers which are useful in the diagnosis and prognosis of GC. However, their sensitivity and specificity in GC remain unsatisfactory. Identification of cancer diagnosed-biomarkers would be of great value. OBJECTIVE: Evaluate the diagnostic and prognostic value of LINC00086 and miR-214 in GC. METHODS: In this study, we determined the expression of LINC00086 and miR-214 in GC by qRT-PCR. Additionally, we investigated the relationship between various clinicopathological features of GC patients and LINC00086 or miR-214 expression, and evaluated the diagnostic and prognostic value of LINC00086 and miR-214 in GC. RESULTS: In this study, we found that plasma LINC00086 expression was significantly lower, whereas plasma miR-214 expression was significantly higher in GC patients than in normal individuals. LINC00086 and miR-214 exhibited high sensitivity and specificity in diagnosing GC. Additionally, GC patients with low LINC00086 or high miR-214 expression were likely to have larger tumors, lymphatic metastasis, larger TNM stage, and higher CEA and CA19-9 levels. Moreover, GC patients with low LINC00086 or high miR-214 expression showed lower survival rates. Lymphatic metastasis, LINC00086, and miR-214 are independent factors affecting patient diagnosis. CONCLUSIONS: LINC00086 and miR-214 are potentially diagnostic and prognostic markers for GC.

Identification of SEPP1 polymorphisms is not a genetic risk factor for preeclampsia in Chinese Han women: A clinical trial and experimental study.

BACKGROUND: SEPP1 encodes selenoprotein P, which involved in oxidative stress and plays an important role in the development of preeclampsia (PE). The aim of this study was to investigate the association between PE and genetic variants of SEPP1 in Chinese Han women. METHODS: In all, 2434 unrelated pregnant women were recruited, including 1034 PE cases and 1400 normal pregnant controls. TaqMan allelic discrimination real-time PCR method was used to genotype the 2 polymorphisms of rs7579 and rs230813 in SEPP1. RESULTS: No statistically significant difference in genotypic or allelic frequencies were found at the 2 genetic variants in SEPP1 between PE patients and controls (rs7579: genotype χ = 2.417, P = .299 and allele χ = 0.197, P = .761, odds ratio 1.049, 95% confidence interval 0.744-1.151; rs230813: genotype χ = 3.273, P = .195 and allele χ = 0.252, P = .615, odds ratio 0.971, 95% confidence interval 0.864-1.091). There were also no statistically significant differences in genetic distributions between mild/severe PE or early/late-onset PE and control subgroups. CONCLUSION: Our data indicate that the 2 genetic variants of rs7579 and rs230813 in SEPP1 may not play a role in the pathogenesis of PE in Chinese Han Women.

Analytical modeling and tolerance analysis of a linear variable filter for spectral order sorting.

This paper proposes an innovative method to overcome the low production rate of current linear variable filter (LVF) fabrication. During the fabrication process, a commercial coater is combined with a local mask on a substrate. The proposed analytical thin film thickness model, which is based on the geometry of the commercial coater, is developed to more effectively calculate the profiles of LVFs. Thickness tolerance, LVF zone width, thin film layer structure, transmission spectrum and the effects of variations in critical parameters of the coater are analyzed. Profile measurements demonstrate the efficacy of local mask theory in the prediction of evaporation profiles with a high degree of accuracy.

Finite element analysis of dental implant neck effects on primary stability and osseointegration in a type IV bone mandible.

The purpose of this study is to investigate the effect of implant neck design and cortical bone thickness by means of 3-D linearly elastic finite element analysis and to analyze primary and secondary stability of clinical evidence based on micromotion and principal stress. Four commercial dental implants, comparable in size, for a type IV bone and mandibular segments were created. Various parameters were considered, including the osseointegration condition (non- and full bonded), force direction (vertical and horizontal) and cortical bone thickness (0.3, 0.5 and 1mm). The force was considered a static load applied at the top of the platform. The magnitudes of the vertical and horizontal loading direction were 500 N and 250 N. Micromotion and principal stresses were employed to evaluate the failure of osseointegration and bone overloading, respectively. The results show that Maximum stress of the peri-implant bone decreased as cortical bone thickness increased. The stress concentration regions were located at the implant neck between the cortical bone and cancellous bone. The micromotion level in full osseointegration is less than that in non-osseointegration and it also decreases as a increasing of cortical bone thickness. Consequently, cortical bone thickness is a key factor for primary stability.

[On mistakes in contemporary literatures of extra points in China].

Contemporary literatures which are taken as the base of literature study of extra points are insufficient and lack of reliability. The foundation of study is very weak. Based on abundant firsthand materials, analyses are made on the major problems of confounded names and locations, unclear quotation and source of reference in the study of contemporary literatures of extra points. Meanwhile, methods and way of thinking for solving the above mentioned problems are discussed in this article as well.

[Study on the title of Chongbian Yijingxiaoxue].

Chongbian Yijingxiaoxue was compiled by Jitian Zongxun in 1590 - 1610. The existing editions included a carved edition and copied edition, then came the photocopy. There was a chapter entitled 'Loujing Xuefa' which recorded 29 extrameridian points. This chapter was compiled into a verse in Volume 3 of Chongbian Yijingxiaoxue. As the book had always been confused with Yijing Xiaoxue, written by Liu Chun in the Ming Dynasty, its literature value increased and became necessary in literature research on extrameridian points. This kind of error was related to people's credulity and blindness on the authority of classical books and 'momentum of literature error'.

[False dissemination of "treatment of hemorrhoids by acupuncture of Kongzui (LU 6)" and its unfavorable influence on modern acupuncture].

"Treatment of hemorrhoids by acupuncture of Kongzui (LU 6)" has been considered to be an example of new discovery in current clinical acupuncture practice and has produced a significant influence on domestic and foreign textbooks of acupuncturology and the health care of the people. After comprehensively analyzing this known saying, it was found that the so-called "new discovery" which has been inherited for decades of years is in fact derived from incorrect dissemination, being lack of evidence of both literature and clinical practice.

[Texture research on the circulating editions and it's composition of Dou tai shi zhen jing (the acupuncture canon of Dou tai shi)].

Dou tai shi zhen jing (the acupuncture canon of Dou tai shi) was an ancient book of acupuncture and moxibustion of Yuan dynasty which had two kinds of transcript of Qing dynasty and the Republic of China. The transcript of Qing dynasty entitled "Mi chao yang shi jia chuan zhen jing tu" (The secret note of acupuncture canon picture of Yang's family heritage). The transcript of the Republic of China was copied together with Yu long ge, so entitled Yu long ge. By making texture research, the two kinds of transcripts were actually different circulating editions of Dou tai shi zhen jing, but the exact title was still uncertain. The author of this book was not Dou Han-qing himself. It should be arranged by Dou's successor and annotated by his descendants. The transcript of Qing dynasty consisted of text, annotation, variorum and headnote. Because Dou tai shi zhen jing was a single conclusion based on clinical practice of acupoint after Zhen quan zhen jing (the acupuncture canon of Zhen quan), so it had high documentary and practical values.