ABSTRACT
OBJECTIVE: To observe the effects of Compound Danshen Tablets (CDST) on spatial cognition and expression of brain b-amyloid precursor protein (beta-APP) in a rat model of Alzheimer's disease. METHODS: The rat model of Alzheimer's disease (AD) was established using D-galactose to cause subacute aging combined with Meynert nucleus damage. Rat behavior was monitored using the Morris water maze, and the expression of beta-APP in rat brain tissue was detected via immunohistochemistry. RESULTS: CDST significantly improved spatial cognition and decreased beta-APP expression in the cortex and hippocampus (P < 0.05, P < 0.01). CONCLUSIONS: CDST can significantly improve spatial cognition in a rat model of AD. This observation is possibly related to a reduction in beta-APP expression in the rat brain.
Subject(s)
Alzheimer Disease/drug therapy , Amyloid beta-Protein Precursor/metabolism , Brain/metabolism , Cognition/drug effects , Drugs, Chinese Herbal/administration & dosage , Salvia miltiorrhiza/chemistry , Alzheimer Disease/metabolism , Alzheimer Disease/psychology , Amyloid beta-Protein Precursor/genetics , Animals , Brain/drug effects , Disease Models, Animal , Female , Humans , Male , RatsABSTRACT
AIM: To investigate the mechanism of NOR(1) gene, a novel gene associated with liver cancer. METHODS: NOR(1) was introduced into HepG2 cells by liposome transfection. After staining and image analysis, 6 differential expression spots which were up-regulated in NOR(1) transfected cells were isolated and identified by two-dimensional polyacrylamide gel eletrophoresis (2D PAGE) and MALDI-TOF. RESULTS: The proteins which included zinc finger protein, tumor necrosis factor receptor, and protein tyrosine phosphatase receptor were involved in gene transcription and signal transduction associated with cancer. CONCLUSION: NOR(1) gene may have some effects on liver cancer by up-regulating the expression of these proteins.
Subject(s)
Membrane Transport Proteins/metabolism , Blotting, Northern , Electrophoresis, Gel, Two-Dimensional , Gene Expression Regulation, Neoplastic , Hep G2 Cells , Humans , Liver Neoplasms , Membrane Transport Proteins/genetics , Receptors, Tumor Necrosis Factor/genetics , Receptors, Tumor Necrosis Factor/metabolism , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
OBJECTIVE: To analyze the effects of cholesterol ester transfer protein (CETP) Taq1B polymorphism on the response of serum HDL-C to dietary factors in hyperlipidemia patients. METHODS: All 141 hyperlipidemia residents in a community in Shanghai were investigated by a 3-day diet questionnaire. The serum lipids were analyzed, and the polymorphism of CETP gene was detected by PCR-RFLP method. The correlation between dietary factors and serum HDL-C in different genotype was analyzed when considering the effects of sex, age and body mass index. RESULT: The HDL-C of hyperlipidemia patients was affected by dietary factors and CETP gene Taq1B polymorphism. The strength of the correlation between dietary factors and serum HDL-C was different among the genotype groups. The relation was shown closer in B(2)B(2) subjects than in B(1)B(1). CONCLUSION: The Taq1B CETP gene polymorphism should be a strong determinant of HDL-C in hyperlipidemia patients, and might contribute to the heterogeneity in HDL-C response to dietary intervention.
Subject(s)
Cholesterol Ester Transfer Proteins/genetics , Cholesterol, HDL/blood , Hyperlipidemias/genetics , Diet , Disease Susceptibility , Female , Gene Frequency , Genotype , Humans , Hyperlipidemias/blood , Male , Middle Aged , Polymorphism, Single NucleotideABSTRACT
OBJECTIVE: To study the association of gene polymorphism at cholesterol ester transfer protein (CETP) locus with obesity and response to dietary intervention in obesity. METHODS: The PCR-PFLP method was used to detect the polymorphism of CETP gene of 340 adults in Shanghai. The levels of serum lipid profile, including TG, TC, HDL and LDL were analyzed. Obesity was selected to dietary intervention. RESULTS: (1)The genotype frequencies of CETP-TaqIB B1 B1, B1 B2 and B2 B2 were 35.6%, 47.9% and 16.5% respectively, which were in agreement with Hardy-Weinberg equilibrium. There was no significant difference in the distribution of genotypes between the obesity group and control group. The result was same after several influence factors controlled. (2) The levels of HDL were significantly different among genotype groups. Subjects for the B2 B2 genotype had the highest HDL levels. The relationship was steady after adjusting several influence factors. (3)Subjects for the B1 B2 genotype had higher HDL level after intervention, which was significantly different to other genotype groups. After adjusting baseline HDL level and gender, genotype didn't effect the change in HDL. CONCLUSION: CETP-TaqIB gene polymorphism influenced serum HDL level. But this gene polymorphism at CETP locus wasn't especial in adult obesity. Baseline HDL level influenced the change in HDL response to dietary intervention in three genotype groups.
Subject(s)
Cholesterol Ester Transfer Proteins/genetics , Dietary Fats/administration & dosage , Obesity/genetics , Polymorphism, Genetic , Aged , Female , Genotype , Humans , Lipoproteins, HDL/blood , Male , Middle Aged , Obesity/metabolism , Polymerase Chain Reaction/methods , Polymorphism, Restriction Fragment LengthABSTRACT
Plasma cholesteryl ester transfer protein (CETP) mediates the transfer of cholesteryl ester (CE) from high density lipoprotein (HDL) to apolipoprotein B-containing lipoproteins. It plays a critical role in reverse cholesterol transport (RCT). Genetic CETP deficiency is the main cause of HALP. Mass research results demonstrate that Genetic CETP deficiency is relative to atherosclerosis. The current review focused on the relationship between cholesteryl ester transfer protein and atherosclerosis.
