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1.
ACS Appl Mater Interfaces ; 12(29): 33386-33396, 2020 Jul 22.
Article in English | MEDLINE | ID: mdl-32589393

ABSTRACT

The continuous emission of nitrous oxides contributes to the overall air pollution and deterioration of air quality. In particular, an effective NO2 sensor capable of low concentration detection for continuous monitoring is demanded for safety, health, and wellbeing. The sensing performance of a metal oxide-based sensor is predominantly influenced by the availability of surface area for O2 adsorption and desorption, efficient charge transport, and size or thickness of the sensing layer. In this study, we utilized anodic one-dimensional (1D) TiO2 nanotube layers of 5 µm thick which offer large surface area and unidirectional electron transport pathway as a platform to accommodate thin SnO2 coatings as a sensing layer. Conformal and homogeneous SnO2 coatings across the entire inner and outer TiO2 nanotubes were achieved by atomic layer deposition with a controlled thickness of 4, 8, and 16 nm. The SnO2-coated TiO2 nanotube layers attained a higher sensing response than a reference Figaro SnO2 sensor. Specifically, the 8 nm SnO2-coated TiO2 nanotube layer has recorded up to ten-fold enhancement in response as compared to the blank nanotubes for the detection of 1 ppm NO2 at an operating temperature of 300 °C with 0.5 V applied bias. This is attributed to the SnO2/TiO2 heterojunction effect and controlled SnO2 thickness within the range of the Debye length. We demonstrated in this work, a tailored large surface area platform based on 1D nanotubes with thin active coatings as an efficient approach for sensing applications and beyond.

2.
Sci Rep ; 10(1): 6925, 2020 04 24.
Article in English | MEDLINE | ID: mdl-32332774

ABSTRACT

Optofluidic devices combining optics and microfluidics have recently attracted attention for biomolecular analysis due to their high detection sensitivity. Here, we show a silicon chip with tubular microchannels buried inside the substrate featuring temperature gradient (∇T) along the microchannel. We set up an optical fluorescence system consisting of a power-modulated laser light source of 470 nm coupled to the microchannel serving as a light guide via optical fiber. Fluorescence was detected on the other side of the microchannel using a photomultiplier tube connected to an optical fiber via a fluorescein isothiocyanate filter. The PMT output was connected to a lock-in amplifier for signal processing. We performed a melting curve analysis of a short dsDNA - SYBR Green I complex with a known melting temperature (TM) in a flow-through configuration without gradient to verify the functionality of the proposed detection system. We then used the segmented flow configuration and measured the fluorescence amplitude of a droplet exposed to ∇T of ≈ 2.31 °C mm-1, determining the heat transfer time as ≈ 554 ms. The proposed platform can be used as a fast and cost-effective system for performing either MCA of dsDNAs or for measuring protein unfolding for drug-screening applications.

3.
Sensors (Basel) ; 19(24)2019 Dec 05.
Article in English | MEDLINE | ID: mdl-31817539

ABSTRACT

Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.

4.
PLoS One ; 14(3): e0214066, 2019.
Article in English | MEDLINE | ID: mdl-30901347

ABSTRACT

Selenium nanoparticle modified surfaces attract increasing attention in the field of tissue engineering. Selenium exhibits strong anticancer, antibacterial and anti-inflammatory properties and it maintains relatively low off-target cytotoxicity. In our paper, we present the fabrication, characterization and cytocompatibility of titanium oxide (TiO2) nanotube surface decorated with various surface densities of chemically synthesized selenium nanoparticles. To evaluate antibacterial and anti-cancer properties of such nanostructured surface, gram negative bacteria E. coli, cancerous osteoblast like MG-63 cells and non-cancerous fibroblast NIH/3T3 were cultured on designed surfaces. Our results suggested that selenium nanoparticles improved antibacterial properties of titanium dioxide nanotubes and confirmed the anticancer activity towards MG-63 cells, with increasing surface density of nanoparticles. Further, the selenium decorated TiO2 nanotubes suggested deteriorating effect on the cell adhesion and viability of non-cancerous NIH/3T3 cells. Thus, we demonstrated that selenium nanoparticles decorated TiO2 nanotubes synthesized using sodium selenite and glutathione can be used to control bacterial infections and prevent the growth of cancerous cells. However, the higher surface density of nanoparticles adsorbed on the surface was found to be cytotoxic for non-cancerous NIH/3T3 cells and thus it might complicate the integration of biomaterial into the host tissue. Therefore, an optimal surface density of selenium nanoparticles must be found to effectively kill bacteria and cancer cells, while remaining favorable for normal cells.


Subject(s)
Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Nanoparticles , Nanotubes , Selenium/pharmacology , Titanium/pharmacology , Animals , Anti-Bacterial Agents/chemistry , Antineoplastic Agents/chemistry , Cell Line, Tumor , Escherichia coli/drug effects , Escherichia coli Infections/drug therapy , Humans , Mice , NIH 3T3 Cells , Nanoparticles/chemistry , Nanoparticles/ultrastructure , Nanotubes/chemistry , Nanotubes/ultrastructure , Neoplasms/drug therapy , Osteoblasts/drug effects , Selenium/chemistry , Titanium/chemistry
5.
J Biomed Nanotechnol ; 14(8): 1505-1514, 2018 Aug 01.
Article in English | MEDLINE | ID: mdl-29903065

ABSTRACT

A non-viral tool for the delivery of nucleic acids termed magnetofection was recently developed as a promising transgenic technique with high transfection efficiency for gene delivery into mammalian cells. Despite the fact that transfection efficiency was the objective in the past, the post-transfection cell morphology and the essential gigaseal formation between cells and patch clamp glass electrodes have not been studied in detail. The cell viability and fluorescent response of Accelerated Sensor of Action Potentials (ASAP1) were studied in somatic HEK293 cells with respect to preserving physiological cell behavior and morphology. The DNA vector (pcDNA3.1/Puro-CAG-ASAP1) was intracellularly delivered by DNA/polyethyleneimine/magnetic nanoparticles and the transfection protocols varied in complex formations were optimized with respect to transfection rate, cytotoxicity of modified nanoparticles and essential gigaseal formation needed for patch clamp technique. A patch clamp study of transfected cells was carried out 72 hours post-transfection. Our results showed the best complex formation in order DNA/magnetic nanoparticle/polyethyleneimine that provides 51.82% transfection efficiency, 83.45% of patch clamp applicable cells, and 90.15% of gigasealed patch clamp applicable cells. A significant difference in fluorescent response of transfected cells was not found compared to control. Thus, these observations suggested that a large amount of the cells were able to create a gigaseal with a glass electrode 72 hours from transfection despite the lower transfection efficiencies.


Subject(s)
Magnetite Nanoparticles , HEK293 Cells , Humans , Polyethyleneimine , Transfection
6.
Anal Biochem ; 539: 22-28, 2017 12 15.
Article in English | MEDLINE | ID: mdl-28970072

ABSTRACT

The main mechanism of action of platinum-based cytostatic drugs - cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 µg/mL), oxaliplatin (5.23 µg/mL), and carboplatin (71.11 µg/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 µg/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of µg per 5 µg of DNA.


Subject(s)
Cytostatic Agents/chemistry , DNA/chemistry , Platinum/chemistry , Sequence Analysis, DNA , Carboplatin/chemistry , Cisplatin/chemistry , DNA/analysis , DNA/metabolism , DNA Adducts/chemistry , DNA Adducts/metabolism , Mass Spectrometry , Organoplatinum Compounds/chemistry , Oxaliplatin , Polymerase Chain Reaction
7.
Sensors (Basel) ; 17(3)2017 Mar 10.
Article in English | MEDLINE | ID: mdl-28287435

ABSTRACT

The aim of this review is to summarize the recent progress in the fabrication of efficient nanostructured polymer-based sensors with special focus on polypyrrole. The correlation between physico-chemical parameters, mainly morphology of various polypyrrole nanostructures, and their sensitivity towards selected gas and volatile organic compounds (VOC) is provided. The different approaches of polypyrrole modification with other functional materials are also discussed. With respect to possible sensors application in medicine, namely in the diagnosis of diseases via the detection of volatile biomarkers from human breath, the sensor interaction with humidity is described as well. The major attention is paid to analytes such as ammonia and various alcohols.

8.
Sensors (Basel) ; 15(5): 12080-91, 2015 May 22.
Article in English | MEDLINE | ID: mdl-26007745

ABSTRACT

The paper presents a new approach in the field of precise electrolytic conductivity measurements with planar thin- and thick-film electrodes. This novel measuring method was developed for measurement with comb-like electrodes called interdigitated electrodes (IDEs). Correction characteristics over a wide range of specific conductivities were determined from an interface impedance characterization of the thick-film IDEs. The local maximum of the capacitive part of the interface impedance is used for corrections to get linear responses. The measuring frequency was determined at a wide range of measured conductivity. An iteration mode of measurements was suggested to precisely measure the conductivity at the right frequency in order to achieve a highly accurate response. The method takes precise conductivity measurements in concentration ranges from 10(-6) to 1 M without electrode cell replacement.

9.
Sensors (Basel) ; 15(2): 2644-61, 2015 Jan 26.
Article in English | MEDLINE | ID: mdl-25629702

ABSTRACT

Vertically aligned multi-walled carbon nanotubes (VA-MWCNTs) with an average diameter below 80 nm and a thickness of the uniform VA-MWCNT layer of about 16 µm were grown in microwave plasma torch and tested for selected functional properties. IR absorption important for a construction of bolometers was studied by Fourier transform infrared spectroscopy. Basic electrochemical characterization was performed by cyclic voltammetry. Comparing the obtained results with the standard or MWCNT­modified screen-printed electrodes, the prepared VA-MWCNT electrodes indicated their high potential for the construction of electrochemical sensors. Resistive CNT gas sensor revealed a good sensitivity to ammonia taking into account room temperature operation. Field emission detected from CNTs was suitable for the pressure sensing application based on the measurement of emission current in the diode structure with bending diaphragm. The advantages of microwave plasma torch growth of CNTs, i.e., fast processing and versatility of the process, can be therefore fully exploited for the integration of surface-bound grown CNTs into various sensing structures.

10.
Sensors (Basel) ; 15(1): 1479-95, 2015 Jan 14.
Article in English | MEDLINE | ID: mdl-25594591

ABSTRACT

A non-invasive solution for monitoring of the activity and dehydration of organisms is proposed in the work. For this purpose, a wireless standalone chemical sensor platform using two separate measurement techniques has been developed. The first approach for activity monitoring is based on humidity measurement. Our solution uses new humidity sensor based on a nanostructured TiO2 surface for sweat rate monitoring. The second technique is based on monitoring of potassium concentration in urine. High level of potassium concentration denotes clear occurrence of dehydration. Furthermore, a Wireless Body Area Network (WBAN) was developed for this sensor platform to manage data transfer among devices and the internet. The WBAN coordinator controls the sensor devices and collects and stores the measured data. The collected data is particular to individuals and can be shared with physicians, emergency systems or athletes' coaches. Long-time monitoring of activity and potassium concentration in urine can help maintain the appropriate water intake of elderly people or athletes and to send warning signals in the case of near dehydration. The created sensor system was calibrated and tested in laboratory and real conditions as well. The measurement results are discussed.


Subject(s)
Dehydration/diagnosis , Monitoring, Physiologic/instrumentation , Calibration , Computer Communication Networks/instrumentation , Electrodes , Equipment Design , Female , Humans , Humidity , Male , Nanostructures/ultrastructure , Titanium/chemistry , Wireless Technology/instrumentation , X-Ray Diffraction
11.
Photochem Photobiol ; 91(2): 417-23, 2015.
Article in English | MEDLINE | ID: mdl-25476270

ABSTRACT

The present work is aimed to synthesize CdTe/ZnSe core/shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in in vitro imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time-consuming method. Subsequently, these QDs were characterized by spectrofluorimetry, Transmission Electron Microscopy, X-ray fluorescence analysis and Dynamic Light Scattering measurement. A blueshifting of the emission was found when ZnSe was deposited on CdTe QDs. The QDs showed its fluorescence emission quantum yields up to 25%. They were applied into chicken embryos and breast muscle tissues to study their efficiency in in vitro imaging. All the QDs of different color were able to visualize in in vitro imaging. The highest fluorescence intensity was detected in the case of red QDs prepared at 100°C. The green and red QDs were possible to detect up to the depth of 3 and 4 mm of the tissue, respectively.


Subject(s)
Cadmium Compounds/chemistry , Embryo, Nonmammalian/ultrastructure , Molecular Imaging/methods , Muscle, Skeletal/ultrastructure , Quantum Dots/chemistry , Selenium Compounds/chemistry , Tellurium/chemistry , Zinc Compounds/chemistry , Animals , Chickens , Microwaves , Spectrometry, Fluorescence , Temperature
12.
Methods Mol Biol ; 1129: 381-96, 2014.
Article in English | MEDLINE | ID: mdl-24648089

ABSTRACT

In 1957, protein rich in cysteine able to bind cadmium was isolated from horse kidney and named as metallothionein according to its structural properties. Further, this protein and metallothionein-like proteins have been found in tissues of other animal species, yeasts, fungi and plants. MT is as a potential cancer marker in the focus of interest, and its properties, functions, and behavior under various conditions are intensively studied. Our protocol describes separation of two major mammalian isoforms of MT (MT-1 and MT-2) using capillary electrophoresis (CE) coupled with UV detector. This protocol enables separation of MT isoforms and studying of their basic behavior as well as their quantification with detection limit in units of ng per µL. Sodium borate buffer (20 mM, pH 9.5) was optimized as a background electrolyte, and the separation was carried out in fused silica capillary with internal diameter of 75 µm and electric field intensity of 350 V/cm. Optimal detection wavelength was 254 nm.


Subject(s)
Electrophoresis, Capillary/methods , Proteins/analysis , Amino Acid Sequence , Molecular Sequence Data , Proteins/chemistry , Spectrophotometry, Ultraviolet
13.
Electrophoresis ; 35(2-3): 393-404, 2014 Feb.
Article in English | MEDLINE | ID: mdl-23868417

ABSTRACT

Microfluidic techniques have been developed intensively in recent years due to lower reagent consumption, faster analysis, and possibility of the integration of several analytical detectors into one chip. Electrochemical detectors are preferred in microfluidic systems, whereas liposomes can be used for amplification of the electrochemical signals. The aim of this study was to design a nanodevice for targeted anchoring of liposome as transport device. In this study, liposome with encapsulated Zn(II) was prepared. Further, gold nanoparticles were anchored onto the liposome surface allowing binding of thiol moiety-modified molecules (DNA). For targeted capturing of the transport device, DNA loops were used. DNA loops were represented by paramagnetic microparticles with oligo(DT)25 chain, on which a connecting DNA was bound. Capturing of transport device was subsequently done by hybridization to the loop. The individual steps were analyzed by electrochemistry and UV/Vis spectrometry. For detection of Zn(II) encapsulated in liposome, a microfluidic system was used. The study succeeded in demonstrating that liposome is suitable for the transport of Zn(II) and nucleic acids. Such transporter may be used for targeted binding using DNA anchor system.


Subject(s)
Liposomes/chemistry , Nanostructures/chemistry , Nucleic Acids/chemistry , Gold/chemistry , Liposomes/metabolism , Liposomes/ultrastructure , Metal Nanoparticles/chemistry , Microfluidic Analytical Techniques , Nanomedicine/instrumentation , Nanostructures/ultrastructure , Nucleic Acids/metabolism , Zinc/chemistry
14.
Materials (Basel) ; 7(3): 1455-1472, 2014 Feb 25.
Article in English | MEDLINE | ID: mdl-28788525

ABSTRACT

The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected-peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.

15.
Nanoscale Res Lett ; 8(1): 503, 2013 Dec 01.
Article in English | MEDLINE | ID: mdl-24289214

ABSTRACT

A biphasic solvothermal reaction method has been used for the synthesis of TiO2 nanoparticles (NPs). In this method, hydrolysis and nucleation occur at the interface of organic phase (titanium (IV) n-propoxide and stearic acid dissolved in toluene) and water phase (tert-butylamine dissolved in water) resulting in the nucleation of the stearic acid-capped TiO2 NPs. These NPs are hydrophilic due to hydrophobic stearic acid ligands and could be dispersed in toluene, but not in water. These stearic acid-capped TiO2 NPs were surface-modified with 2,3-dimercaptosuccinic acid (DMSA) in order to make them water soluble. The resultant TiO2 NPs were easily redispersed in water without any noticeable aggregation. The Rietveld profile fitting of X-ray diffraction (XRD) pattern of the TiO2 NPs revealed highly crystalline anatase structure. The average crystallite size of TiO2 NPs was calculated to be 6.89 nm, which agrees with TEM results. These results have important implications for the use of TiO2 in biomedical, environmental, and industrial applications.

16.
Sensors (Basel) ; 13(11): 14417-37, 2013 Oct 25.
Article in English | MEDLINE | ID: mdl-24233071

ABSTRACT

In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl2, Zn(his)Cl2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to use) instrumentation and variable setting of flows. Reduction signals of zinc under the strictly defined and controlled conditions (pH, temperature, flow rate, accumulation time and applied potential) were studied. We showed that the increasing concentration of the complexes (Zn(phen)(his)Cl2, Zn(his)Cl2) led to a decrease in the electrochemical signal and a significant shift of the potential to more positive values. The most likely explanation of this result is that zinc is strongly bound in the complex and its distribution on the electrode is very limited. Changing the pH from 3.5 to 5.5 resulted in a significant intensification of the Zn(II) reduction signal. The complexes were also characterized by UV/VIS spectrophotometry, chromatography, and ESI-QTOF mass spectrometry.

17.
Prion ; 7(5): 349-58, 2013.
Article in English | MEDLINE | ID: mdl-24055838

ABSTRACT

A diagnostics of infectious diseases can be done by the immunologic methods or by the amplification of nucleic acid specific to contagious agent using polymerase chain reaction. However, in transmissible spongiform encephalopathies, the infectious agent, prion protein (PrP(Sc)), has the same sequence of nucleic acids as a naturally occurring protein. The other issue with the diagnosing based on the PrP(Sc) detection is that the pathological form of prion protein is abundant only at late stages of the disease in a brain. Therefore, the diagnostics of prion protein caused diseases represent a sort of challenges as that hosts can incubate infectious prion proteins for many months or even years. Therefore, new in vivo assays for detection of prion proteins and for diagnosis of their relation to neurodegenerative diseases are summarized. Their applicability and future prospects in this field are discussed with particular aim at using quantum dots as fluorescent labels.


Subject(s)
Fluorescent Dyes , Neurodegenerative Diseases/diagnosis , Prions/analysis , Quantum Dots , Animals , Brain/pathology , Humans , Models, Molecular , Neurodegenerative Diseases/pathology , Prion Diseases/diagnosis , Prion Diseases/pathology
18.
J Pharm Biomed Anal ; 86: 65-72, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23994762

ABSTRACT

Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step is essential for all of them - isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimized method with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureus and Escherichia coli.


Subject(s)
Bacteriophage lambda/metabolism , Chemistry, Pharmaceutical/methods , Magnetic Phenomena , Silicon Dioxide/analysis , Silicon Dioxide/metabolism , DNA Viruses/metabolism , Particle Size
19.
Viruses ; 5(7): 1719-39, 2013 Jul 15.
Article in English | MEDLINE | ID: mdl-23860384

ABSTRACT

Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads® with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids.


Subject(s)
DNA Barcoding, Taxonomic/methods , Electrochemical Techniques/methods , Influenza A Virus, H5N1 Subtype/enzymology , Magnetics/methods , Neuraminidase/genetics , Point Mutation , Viral Proteins/genetics , Virology/methods , Animals , China , Drug Resistance, Viral , Humans , Influenza A Virus, H5N1 Subtype/classification , Influenza A Virus, H5N1 Subtype/genetics , Mutant Proteins/genetics
20.
Int J Mol Sci ; 14(7): 13497-510, 2013 Jun 27.
Article in English | MEDLINE | ID: mdl-23807507

ABSTRACT

Electrochemical detection of quantum dots (QDs) has already been used in numerous applications. However, QDs have not been well characterized using voltammetry, with respect to their characterization and quantification. Therefore, the main aim was to characterize CdTe QDs using cyclic and differential pulse voltammetry. The obtained peaks were identified and the detection limit (3 S/N) was estimated down to 100 fg/mL. Based on the convincing results, a new method for how to study stability and quantify the dots was suggested. Thus, the approach was further utilized for the testing of QDs stability.


Subject(s)
Cadmium/chemistry , Quantum Dots/chemistry , Tellurium/chemistry , Animals , Cadmium/pharmacology , Chickens , Quantum Dots/therapeutic use , Quantum Dots/ultrastructure , Tellurium/pharmacology
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