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2.
Bioorg Med Chem Lett ; 11(11): 1411-5, 2001 Jun 04.
Article in English | MEDLINE | ID: mdl-11378366

ABSTRACT

A series of aryloxy substituted piperazinones with dual farnesyltransferase/geranylgeranyltransferase-I inhibitory activity was prepared. These compounds were found to have potent inhibitory activity in vitro and are promising agents for the inhibition of Ki-Ras signaling.


Subject(s)
Alkyl and Aryl Transferases/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Piperazines/pharmacology , Drug Design , Enzyme Inhibitors/chemistry , Farnesyltranstransferase , Genes, ras/drug effects , Piperazines/chemistry , Polymers/chemistry , Signal Transduction/drug effects , Structure-Activity Relationship
3.
Bioorg Med Chem Lett ; 11(7): 865-9, 2001 Apr 09.
Article in English | MEDLINE | ID: mdl-11294379

ABSTRACT

A series of 2-arylindole-3-acetamide farnesyl protein transferase inhibitors has been identified. The compounds inhibit the enzyme in a farnesyl pyrophosphate-competitive manner and are selective for farnesyl protein transferase over the related enzyme geranylgeranyltransferase-I. A representative member of this series of inhibitors demonstrates equal effectiveness against HDJ-2 and K-Ras farnesylation in a cell-based assay when geranylgeranylation is suppressed.


Subject(s)
Alkyl and Aryl Transferases/antagonists & inhibitors , Indoleacetic Acids/metabolism , Indoleacetic Acids/pharmacology , Protein Prenylation/drug effects , ras Proteins/metabolism , Alkyl and Aryl Transferases/metabolism , Carrier Proteins/metabolism , Enzyme Inhibitors/chemical synthesis , Enzyme Inhibitors/metabolism , Enzyme Inhibitors/pharmacology , HSP40 Heat-Shock Proteins , Heat-Shock Proteins/metabolism , Humans , Indoleacetic Acids/chemical synthesis , Protein Prenylation/physiology , Structure-Activity Relationship , Tumor Cells, Cultured
4.
J Biol Chem ; 276(27): 24457-65, 2001 Jul 06.
Article in English | MEDLINE | ID: mdl-11274181

ABSTRACT

We have identified and characterized potent and specific inhibitors of geranylgeranyl-protein transferase type I (GGPTase I), as well as dual inhibitors of GGPTase I and farnesyl-protein transferase. Many of these inhibitors require the presence of phosphate anions for maximum activity against GGPTase I in vitro. Inhibitors with a strong anion dependence were competitive with geranylgeranyl pyrophosphate (GGPP), rather than with the peptide substrate, which had served as the original template for inhibitor design. One of the most effective anions was ATP, which at low millimolar concentrations increased the potency of GGPTase I inhibitors up to several hundred-fold. In the case of clinical candidate l-778,123, this increase in potency was shown to result from two major interactions: competitive binding of inhibitor and GGPP, and competitive binding of ATP and GGPP. At 5 mm, ATP caused an increase in the apparent K(d) for the GGPP-GGPTase I interaction from 20 pm to 4 nm, resulting in correspondingly tighter inhibitor binding. A subset of very potent GGPP-competitive inhibitors displayed slow tight binding to GGPTase I with apparent on and off rates on the order of 10(6) m(-)1 s(-)1 and 10(-)3 s(-)1, respectively. Slow binding and the anion requirement suggest that these inhibitors may act as transition state analogs. After accounting for anion requirement, slow binding, and mechanism of competition, the structure-activity relationship determined in vitro correlated well with the inhibition of processing of GGPTase I substrate Rap1a in vivo.


Subject(s)
Alkyl and Aryl Transferases/antagonists & inhibitors , Anions/metabolism , Enzyme Inhibitors/pharmacology , Adenosine Triphosphate/metabolism , Binding, Competitive , Humans , Imidazoles/pharmacology , Kinetics , Models, Chemical , Polyisoprenyl Phosphates/metabolism , Protein Binding , Structure-Activity Relationship
5.
J Antibiot (Tokyo) ; 52(12): 1086-94, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10695671

ABSTRACT

A resorcylic acid lactone, L-783,277, isolated from a Phoma sp. (ATCC 74403) which came from the fruitbody of Helvella acetabulum, is a potent and specific inhibitor of MEK (Map kinase kinase). L-783,277 inhibits MEK with an IC50 value of 4 nM. It weakly inhibits Lck and is inactive against Raf, PKA and PKC. L-783,277 is an irreversible inhibitor of MEK and is competitive with respect to ATP. L-783,290, the trans-isomer of L-783,277, was isolated from the same culture and evaluated together with several semi-synthetic resorcylic acid lactone analogs. A preliminary structure-activity relationship is presented. Several independent cell-based assays have been carried out to study the biological activities of these resorcylic acid lactone compounds and a brief result summary from these studies is presented.


Subject(s)
Enzyme Inhibitors/pharmacology , Lactones/pharmacology , MAP Kinase Kinase Kinase 1 , Protein Serine-Threonine Kinases/antagonists & inhibitors , Resorcinols/pharmacology , Adenosine Triphosphate/pharmacology , Humans , Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/antagonists & inhibitors
6.
J Biol Chem ; 269(9): 6999-7005, 1994 Mar 04.
Article in English | MEDLINE | ID: mdl-8120063

ABSTRACT

E2F is a mammalian transcription factor involved in cell cycle regulation. The retinoblastoma gene product, pRB, binds to E2F in a cell cycle-dependent manner and appears to turn E2F from a transcriptional activator into a repressor. We show here that in vitro binding of pRB has three major effects on the DNA binding properties of E2F affinity-purified from HeLa cells; pRB binding increases the half-life of E2F.DNA complexes 10-15-fold, it reduces E2F specific DNA binding in the presence of nonspecific DNA by sequestering E2F, and it partially reverses the DNA bending induced by E2F. Upon specific DNA binding, E2F induces a DNA bend with a flexure angle of 125 degrees. Both full-length pRB105 and the N-terminally truncated pRB60 bind to the E2F.DNA complex with a Kd,app of 150 pM and reduce the apparent DNA bending to less than 80 degrees. DNA footprinting analysis indicates that the nonspecific DNA binding activity of pRB is not involved in this effect. Our biochemical data suggest that transcriptional activation by E2F may involve DNA bending and that the reversal of bending upon binding of pRB may turn E2F into a repressor.


Subject(s)
Carrier Proteins , Cell Cycle Proteins , DNA-Binding Proteins , DNA/chemistry , DNA/metabolism , Oligodeoxyribonucleotides/metabolism , Retinoblastoma Protein/metabolism , Transcription Factors/metabolism , Base Sequence , Binding Sites , Chromatography, Affinity , Deoxyribonuclease I , E2F Transcription Factors , HeLa Cells , Humans , Kinetics , Molecular Sequence Data , Mutagenesis, Site-Directed , Nucleic Acid Conformation , Plasmids/metabolism , Promoter Regions, Genetic , Protein Binding , Repressor Proteins/metabolism , Retinoblastoma-Binding Protein 1 , Transcription Factor DP1 , Transcription Factors/isolation & purification
7.
Mol Cell Biol ; 13(12): 7802-12, 1993 Dec.
Article in English | MEDLINE | ID: mdl-8246995

ABSTRACT

E2F is a mammalian transcription factor that appears to play an important role in cell cycle regulation. While at least two proteins (E2F-1 and DP-1) with E2F-like activity have been cloned, studies from several laboratories suggest that additional homologs may exist. A novel protein with E2F-like properties, designated E2F-2, was cloned by screening a HeLa cDNA library with a DNA probe derived from the DNA binding domain of E2F-1 (K. Helin, J. A. Lees, M. Vidal, N. Dyson, E. Harlow, and A. Fattaey, Cell 70:337-350, 1992). E2F-2 exhibits overall 46% amino acid identity to E2F-1. Both the sequence and the function of the DNA and retinoblastoma gene product binding domains of E2F-1 are conserved in E2F-2. The DNA binding activity of E2F-2 is dramatically enhanced by complementation with particular sodium dodecyl sulfate-polyacrylamide gel electrophoresis-purified components of HeLa cell E2F, and anti-E2F-2 antibodies cross-react with components of purified HeLa cell E2F. These observations are consistent with a model in which E2F binds DNA as a heterodimer of two distinct proteins, and E2F-2 is functionally and immunologically related to one of these proteins.


Subject(s)
Carrier Proteins , Cell Cycle Proteins , Transcription Factors/genetics , Amino Acid Sequence , Base Sequence , Binding Sites/genetics , Cloning, Molecular , Conserved Sequence , DNA Primers/genetics , DNA, Complementary/genetics , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , E2F Transcription Factors , E2F1 Transcription Factor , E2F2 Transcription Factor , Glutathione Transferase/genetics , HeLa Cells , Humans , Molecular Sequence Data , Protein Conformation , RNA, Messenger/genetics , Recombinant Fusion Proteins/genetics , Retinoblastoma-Binding Protein 1 , Transcription Factor DP1 , Transcription Factors/chemistry , Transcription Factors/metabolism
8.
Proc Natl Acad Sci U S A ; 90(8): 3525-9, 1993 Apr 15.
Article in English | MEDLINE | ID: mdl-8475102

ABSTRACT

E2F is a mammalian transcription factor that appears to play an important role in cell cycle control. DNA affinity column-purified E2F from HeLa cells reproducibly exhibits multiple protein bands when analyzed by SDS/PAGE. After electrophoretic purification, electroelution, and refolding of the individual protein components, the E2F DNA binding activity of the individual proteins was poor. However, upon mixing the individual components together, a dramatic (100- to 1000-fold) increase in specific DNA binding activity was observed. The five protein bands isolated can be separated into two groups based on apparent molecular mass. Optimal reconstitution of activity requires one of the two proteins found in the group of larger molecular mass (approximately 60 kDa) and one of the three proteins in the smaller-sized group (approximately 50 kDa). The reconstituted heterodimer is identical to authentic affinity-purified E2F by three criteria: DNA-binding specificity, DNA pattern, and binding to the retinoblastoma gene product. A recently cloned protein with E2F-like activity, RBP3/E2F-1, is related to the protein components of the group of larger molecular mass, as determined by Western blot analysis and reconstitution experiments. These data suggest that E2F, like many other transcription factors, binds DNA as an oligomeric complex composed of at least two distinct proteins.


Subject(s)
Carrier Proteins/metabolism , Cell Cycle Proteins , DNA-Binding Proteins/metabolism , DNA/metabolism , Transcription Factors/metabolism , Adenoviruses, Human/genetics , Base Sequence , Binding Sites , Blotting, Western , Carrier Proteins/genetics , Carrier Proteins/isolation & purification , Chromatography, Affinity , DNA, Viral/genetics , DNA, Viral/metabolism , DNA-Binding Proteins/isolation & purification , E2F Transcription Factors , Glutathione Transferase/genetics , Glutathione Transferase/isolation & purification , Glutathione Transferase/metabolism , HeLa Cells , Humans , Macromolecular Substances , Molecular Sequence Data , Oligodeoxyribonucleotides/metabolism , Promoter Regions, Genetic , Protein Denaturation , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Retinoblastoma Protein/metabolism , Retinoblastoma-Binding Protein 1 , Substrate Specificity , Transcription Factor DP1 , Transcription Factors/isolation & purification
9.
Mol Cell Biol ; 13(2): 953-60, 1993 Feb.
Article in English | MEDLINE | ID: mdl-7678696

ABSTRACT

Human papillomaviruses (HPVs) are the etiological agents for genital warts and contribute to the development of cervical cancer in humans. The HPV E7 gene product is expressed in these diseases, and the E7 genes from HPV types 16 and 18 contribute to transformation in mammalian cells. Mutation and deletion analysis of this gene suggests that the transforming activity of the protein product resides in the same domain as that which is directly involved in complex formation with the retinoblastoma gene product (pRB). This domain is one of two conserved regions (designated CRI and CRII) shared by E7 and other viral oncoproteins which bind pRB, including adenovirus E1A protein. Binding of HPV type 16 E7 protein to pRB has previously been shown to affect pRB's ability to bind DNA and to form complexes with other cellular proteins. In the current study, we map the functional interaction between E7 protein and pRB by monitoring the association between a 60-kDa version of the pRB, pRB60, and the cellular transcription factor E2F. We observe that CRII of E7 (amino acids 20 to 29), which completely blocks binding of full-length E7 protein, is necessary but not sufficient to inhibit E2F/pRB60 complex formation. While CRI of E1A (amino acids 37 to 55) appears to be sufficient to compete with E2F for binding to pRB60, the equivalent region of E7 is neither necessary nor sufficient. Only E7 fragments that contained both CRII and at least a portion of the zinc-binding domain (amino acids 60 to 98) inhibited E2F/pRB60 complex formation. These results suggest that pRB60 associates with E7 and E2F through overlapping but distinct domains.


Subject(s)
Carrier Proteins , Cell Cycle Proteins , DNA-Binding Proteins , Oncogene Proteins, Viral/metabolism , Retinoblastoma Protein/metabolism , Transcription Factors/metabolism , Amino Acid Sequence , Base Sequence , Binding, Competitive , DNA/metabolism , E2F Transcription Factors , Epitopes , HeLa Cells , Humans , Molecular Sequence Data , Oncogene Proteins, Viral/chemistry , Papillomavirus E7 Proteins , Peptide Fragments/metabolism , Retinoblastoma Protein/chemistry , Retinoblastoma-Binding Protein 1 , Transcription Factor DP1 , Transcription Factors/chemistry
10.
Mol Cell Biol ; 12(5): 1905-14, 1992 May.
Article in English | MEDLINE | ID: mdl-1314947

ABSTRACT

The human papillomavirus E7 gene can transform murine fibroblasts and cooperate with other viral oncogenes in transforming primary cell cultures. One biochemical property associated with the E7 protein is binding to the retinoblastoma tumor suppressor gene product (pRB). Biochemical properties associated with pRB include binding to viral transforming proteins (E1A, large T, and E7), binding to cellular proteins (E2F and Myc), and binding to DNA. The mechanism by which E7 stimulates cell growth is uncertain. However, E7 binding to pRB inhibits binding of cellular proteins to pRB and appears to block the growth-suppressive activity of pRB. We have found that E7 also inhibits binding of pRB to DNA. A 60-kDa version of pRB (pRB60) produced in reticulocyte translation reactions or in bacteria bound quantitatively to DNA-cellulose. Recombinant E7 protein used at a 1:1 or 10:1 molar ratio with pRB60 blocked 50 or greater than 95% of pRB60 DNA-binding activity, respectively. A mutant E7 protein (E7-Ala-24) with reduced pRB60-binding activity exhibited a parallel reduction in its blocking of pRB60 binding to DNA. An E7(20-29) peptide that blocks binding of E7 protein to pRB60 restored the DNA-binding activity of pRB60 in the presence of E7. Peptide E7(2-32) did not block pRB60 binding to DNA, while peptide E7(20-57) and an E7 fragment containing residues 1 to 60 partially blocked DNA binding. E7 species containing residues 3 to 75 were fully effective at blocking pRB60 binding to DNA. These studies indicate that E7 protein specifically blocks pRB60 binding to DNA and suggest that the E7 region responsible for this property lies between residues 32 and 75. The functional significance of these observations is unclear. However, we have found that a point mutation in pRB60 that impairs DNA-binding activity also blocks the ability of pRB60 to inhibit cell growth. This correlation suggests that the DNA-binding activity of retinoblastoma proteins contributes to their biological properties.


Subject(s)
DNA-Binding Proteins/metabolism , DNA/metabolism , Oncogene Proteins, Viral/metabolism , Papillomaviridae/metabolism , Retinoblastoma Protein/metabolism , 3T3 Cells , Amino Acid Sequence , Animals , Cell Division , Chromatography, Affinity , Chromosome Deletion , Cloning, Molecular , DNA-Binding Proteins/genetics , Escherichia coli/genetics , Genes, Retinoblastoma , Genes, Viral , Humans , Kinetics , Mice , Molecular Sequence Data , Oligonucleotide Probes , Oncogene Proteins, Viral/genetics , Papillomaviridae/genetics , Papillomavirus E7 Proteins , Peptides/chemical synthesis , Protein Binding , Protein-Tyrosine Kinases/metabolism , Recombinant Fusion Proteins/metabolism , Recombinant Proteins/metabolism , Retinoblastoma Protein/genetics
11.
J Biol Chem ; 267(12): 7971-4, 1992 Apr 25.
Article in English | MEDLINE | ID: mdl-1569054

ABSTRACT

The retinoblastoma susceptibility gene (RB) encodes a 928-amino acid protein (pRB) that is hypothesized to function in a pathway that restricts cell proliferation. The immortalizing proteins from three distinct DNA tumor viruses (SV40 large T antigen, adenovirus E1a, and human papilloma virus Type 16 E7) have been shown to interact with RB protein through two noncontiguous regions comprised of amino acids 393-572 (domain A) and 646-772 (domain B). We constructed a truncated form of RB (RB p60) that retains these two domains but eliminates the N-terminal 386 amino acids of RB. RB p60 was expressed in Escherichia coli in inclusion bodies. After solubilization, it was refolded in the presence of magnesium chloride, and the active protein was isolated with an E7 peptide affinity column. The protein that elutes from this column is functionally homogenous in its ability to bind immobilized E7 protein. Thermal denaturation studies provide additional evidence for the conformational homogeneity of the isolated protein. This purification scheme allows the isolation of significant amounts of RB p60 protein that is suitable for structural and functional studies.


Subject(s)
Retinoblastoma Protein/isolation & purification , Chromatography, Affinity , Circular Dichroism , DNA/genetics , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Escherichia coli/genetics , Gene Expression , Genes, Bacterial , Hot Temperature , Humans , Oncogene Proteins, Viral/metabolism , Papillomavirus E7 Proteins , Plasmids , Protein Conformation , Protein Denaturation , Retinoblastoma Protein/metabolism
12.
J Biol Chem ; 267(2): 908-12, 1992 Jan 15.
Article in English | MEDLINE | ID: mdl-1309779

ABSTRACT

Complex formation between the human papilloma virus type 16 E7 protein (HPV-16 E7) and the retinoblastoma growth suppressor protein (RB) is believed to contribute to the process of cellular transformation that leads to cervical carcinoma. Genetic analysis of the HPV-16 E7 protein has shown that the segment of E7 homologous to the conserved region 2 of adenovirus 5 E1A protein is involved in both RB binding and E7-mediated cell transformation. We have previously shown that a peptide colinear with HPV-16 E7 residues 21-29 was able to block immobilized species of E7 from binding to RB protein. The current study reports the effects of different chemical modifications of this peptide. One type of modification, methylation of the alpha-amino nitrogens contributed by Leu22, Tyr25, and Leu28, resulted in a 45-fold increase in E7/RB binding antagonist activity. This increased antagonist activity is sequence-specific since methylation of the amino groups contributed by Tyr23, Cys24, or Glu26 resulted in a profound loss of binding antagonist activity. Using a newly developed binding assay we determined that the apparent dissociation constant for recombinant HPV-16 E7 protein binding to recombinant human RB protein is 1.3 nM. The peptide Ac[N-MeLeu22,N-Me-Tyr25,N-MeLeu28]-(21-29)-E7 amide was determined to be a competitive inhibitor of HPV-16 E7 binding to RB with a Ki value of 32 nM.


Subject(s)
Oncogene Proteins, Viral/metabolism , Papillomaviridae/metabolism , Retinoblastoma Protein/metabolism , Amino Acid Sequence , DNA, Viral/genetics , Humans , Methylation , Molecular Sequence Data , Oncogene Proteins, Viral/genetics , Papillomavirus E7 Proteins , Plasmids , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Retinoblastoma Protein/genetics
13.
Nature ; 352(6332): 251-4, 1991 Jul 18.
Article in English | MEDLINE | ID: mdl-1857421

ABSTRACT

The E7 transforming protein of human papilloma virus-16 binds to the retinoblastoma gene product (pRb) through a nine-amino-acid segment of E7 (21-29). This segment of E7 is homologous to the pRb-binding domains of the simian virus 40 large T and adenovirus E1A transforming proteins. Each of these viral transforming proteins bind to the same region of pRb. To isolate cellular proteins that interact with this viral protein-binding domain on pRb, we used recombinant pRb to screen a human complementary DNA expression library. Two cDNAs were isolated that encode retinoblastoma binding proteins (RBP-1 and RBP-2). We report here that these RBP genes exist in separate loci and produce discrete messenger RNAs. The predicted amino-acid sequence of these genes showed no homology to known proteins, but both RBPs contain the pRb binding motif conserved between E7, large T and E1A14. In vitro expression of the RBP cDNAs yielded proteins that specifically bound to pRb. Recombinant E7 protein, the E7 21-29 peptide and the homologous RBP-1 peptide inhibited RBP-pRb binding. Mutations introduced into the putative pRb-binding segment in RBP-1 impaired its binding activity. These studies indicate that the cellular RBP-1, RBP-2 and viral E7 proteins interact with pRb through similar domains.


Subject(s)
Carrier Proteins/genetics , DNA/genetics , Intracellular Signaling Peptides and Proteins , Retinoblastoma Protein/metabolism , Tumor Suppressor Proteins , Amino Acid Sequence , Base Sequence , Blotting, Southern , Carrier Proteins/isolation & purification , Carrier Proteins/metabolism , Cloning, Molecular , DNA/isolation & purification , Female , Humans , Molecular Sequence Data , Placenta/physiology , Pregnancy , Recombinant Proteins/metabolism , Retinoblastoma-Binding Protein 2 , Sequence Homology, Nucleic Acid
14.
J Biol Chem ; 265(18): 10565-73, 1990 Jun 25.
Article in English | MEDLINE | ID: mdl-1693920

ABSTRACT

We have analyzed the processing of the RNA primer for (+) strand DNA synthesis by reverse transcriptase of the human immunodeficiency virus 1. To test for specific RNA cleavage and primer usage, we constructed a 99-base pair RNA-DNA hybrid containing the viral polypurine tract and flanking viral sequences. Although the RNase H activity of reverse transcriptase cleaves the RNA strand into multiple fragments, only two primers are extended in the presence of nucleoside triphosphates. The major RNA primer includes the entire polypurine tract except for the last adenosine and has the sequence 5'-UUUUAAAAGAAAAGGGGGG-3'. The minor primer has the same 3' end but is two nucleotides shorter. In a subsequent processing step reverse transcriptase releases the primer intact via a cleavage at the RNA-DNA junction. RNA cleavage, primer extension, and primer removal can take place in a single reaction. However, specificity does not require coupling of the three steps and is preserved in the individual reactions. The polypurine primer is generated and removed after its elongation in the absence of DNA synthesis. Furthermore, the polypurine primer is selected among the several RNA fragments available and extended by reverse transcriptase as well as by p51, a short form of reverse transcriptase lacking RNase H activity.


Subject(s)
DNA Replication , HIV-1/enzymology , RNA, Viral/genetics , RNA-Directed DNA Polymerase/metabolism , Base Sequence , Cloning, Molecular , DNA, Viral/genetics , Escherichia coli/genetics , HIV-1/genetics , Molecular Sequence Data , Promoter Regions, Genetic , RNA-Directed DNA Polymerase/genetics , Recombinant Proteins/metabolism , Transcription, Genetic
15.
J Biol Chem ; 264(8): 4669-78, 1989 Mar 15.
Article in English | MEDLINE | ID: mdl-2466838

ABSTRACT

We have analyzed the kinetics of DNA synthesis catalyzed by reverse transcriptase from human immunodeficiency virus 1 (HIV-1). Reverse transcriptase, overproduced in Escherichia coli and purified to homogeneity, has polymerase and RNase H activity. Reverse transcriptase forms a stable complex with poly(rA).oligo(dT) primer-templates in the absence of Mg2+ and dTTP with an equilibrium dissociation constant of 3 nM. Synthesis from these preformed complexes can be initiated, and restricted to a single processive cycle, by the simultaneous addition of Mg2+, dTTP, and excess competitor RNA. Preformed complexes decay with a maximal half-life of 2-3 min. Synthesis on poly(rA) templates is processive with an incorporation rate of 10-15 nucleotides/s at 37 degrees C. Processivity varies widely with the template used, increasing from a few to greater than 300 nucleotides in the order: poly(dA) less than double-stranded DNA less than single-stranded DNA less than single-stranded RNA less than poly(rA). On double-stranded DNA reverse transcriptase catalyzes limited strand-displacement synthesis of up to 50 nucleotides. On RNA-DNA hybrids significant DNA synthesis is observed only after degradation of the RNA strand by the RNase H activity of reverse transcriptase. Intermolecular strand switching occurs with poly(rA) templates. At low ionic strength reverse transcriptase can use multiple templates with a single primer, leading to products of greater than template length. Reverse transcriptase and primer do not have to dissociate during the exchange of template strands, thus allowing processive DNA synthesis across template borders.


Subject(s)
DNA, Viral/biosynthesis , HIV/enzymology , RNA-Directed DNA Polymerase/metabolism , DNA, Single-Stranded/metabolism , Kinetics , Magnesium/pharmacology , Nucleic Acid Hybridization , Oligodeoxyribonucleotides/metabolism , Poly A/metabolism , RNA/metabolism , Templates, Genetic , Thymine Nucleotides/pharmacology
16.
J Biol Chem ; 263(27): 13549-56, 1988 Sep 25.
Article in English | MEDLINE | ID: mdl-2843524

ABSTRACT

Escherichia coli has a unique enzyme, deoxyguanosine triphosphate triphosphohydrolase (dGTPase) that cleaves dGTP into deoxyguanosine and tripolyphosphate. An E. coli mutant, optA1, has a 50-fold increased level of the dGTPase (Beauchamp, B.B., and Richardson, C.C. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 2563-2567). Successful infection of E. coli optA1 by bacteriophage T7 is dependent on a 10-kDa protein encoded by gene 1.2 of the phage. In this report we show that the gene 1.2 protein is a specific inhibitor of the E. coli dGTPase. Gene 1.2 protein inhibits dGTPase activity by forming a complex with the dGTPase with an apparent stoichiometry of two monomers of gene 1.2 protein/tetramer of dGTPase. The interaction is reversible with a half-life of the complex of 30 min and an apparent binding constant Ki of 35 nM. The binding of inhibitor of dGTPase is cooperative, indicating allosteric interactions between dGTPase subunits with a Hill coefficient of 1.7. The interaction is modulated differentially by DNA, RNA, and deoxyguanosine mono-, di-, and triphosphate. Both the binding of the substrate dGTP and of the inhibitor gene 1.2 protein induce conformational changes in dGTPase. The conformation of the enzyme in the presence of saturating concentrations of dGTP virtually prevents the association with, and the dissociation from, gene 1.2 protein.


Subject(s)
Escherichia coli/enzymology , Phosphoric Monoester Hydrolases/antagonists & inhibitors , Viral Proteins/pharmacology , Chromatography, Affinity , DNA/metabolism , Deoxyguanine Nucleotides/metabolism , Deoxyguanine Nucleotides/pharmacology , Kinetics , Macromolecular Substances , Magnesium/pharmacology , Manganese/pharmacology , Phosphoric Monoester Hydrolases/metabolism , T-Phages , Viral Proteins/metabolism
17.
J Biol Chem ; 262(33): 16212-23, 1987 Nov 25.
Article in English | MEDLINE | ID: mdl-3316214

ABSTRACT

Bacteriophage T7 gene 5 protein has been purified to apparent homogeneity from cells overexpressing its gene several hundred-fold. Gene 5 protein is a DNA polymerase with low processivity; it dissociates from the primer-template after catalyzing the incorporation of 1-50 nucleotides, depending on the salt concentration. Escherichia coli thioredoxin, a host protein that is tightly associated with the gene 5 protein in phage-infected cells, is not required for this activity. Thioredoxin acts as an accessory protein to bestow processivity on the polymerizing reaction; DNA synthesis catalyzed by the gene 5 protein-thioredoxin complex on a single-stranded DNA template can polymerize thousands of nucleotides without dissociation. Conditions that increase the stability of secondary structures in the template (i.e., low temperature or high ionic strength) decrease the processivity. E. coli single-stranded DNA-binding protein stimulates both the rate of elongation and the processivity of the gene 5 protein-thioredoxin complex.


Subject(s)
DNA-Directed DNA Polymerase/metabolism , Escherichia coli/metabolism , T-Phages/metabolism , Viral Proteins/metabolism , Escherichia coli/genetics , Genetic Complementation Test , Kinetics , Plasmids , T-Phages/genetics , Viral Proteins/genetics , Viral Proteins/isolation & purification
18.
J Biol Chem ; 262(33): 16224-32, 1987 Nov 25.
Article in English | MEDLINE | ID: mdl-3316215

ABSTRACT

The DNA polymerase activity induced after bacteriophage T7 infection of Escherichia coli is found in a complex of two proteins, the T7 gene 5 protein and a host protein, thioredoxin. Gene 5 protein is a DNA polymerase and a 3' to 5' exonuclease. Thioredoxin binds tightly to the gene 5 protein and increases the processivity of polymerization some 1000-fold. Gene 5 protein forms a short-lived complex with the primer-template, poly(dA).oligo(dT), in the absence of Mg2+ and nucleotides. Thioredoxin increases the half-life of the preformed primer-template-polymerase complex from less than a second to approximately 5 min. The dissociation is accelerated by excess single-stranded DNA in an apparent second order reaction, indicating direct transfer of polymerase between DNA fragments. Thioredoxin also reduces the equilibrium dissociation constant, Kd, of the gene 5 protein -poly(dA).oligo(dT) complex 20- to 80-fold. The salt dependence of Kd indicates that thioredoxin stabilizes the primer-template-polymerase complex mainly through additional charge-charge interactions, increasing the estimated number of interactions from 2 to 7. The affinity of gene 5 protein for single-stranded DNA is at least 1000-fold higher than for double-stranded DNA and is little affected by thioredoxin. Under conditions of steady state synthesis the effect of thioredoxin on the polymerization rate is determined by two competing factors, an increase in processivity and a decrease of the dissociation rate of polymerase and replicated template.


Subject(s)
Bacterial Proteins/metabolism , DNA-Directed DNA Polymerase/metabolism , Escherichia coli/metabolism , T-Phages/metabolism , Thioredoxins/metabolism , Viral Proteins/metabolism , Escherichia coli/genetics , Kinetics , T-Phages/genetics , Templates, Genetic , Viral Proteins/genetics
19.
J Biol Chem ; 261(32): 15006-12, 1986 Nov 15.
Article in English | MEDLINE | ID: mdl-3533931

ABSTRACT

DNA polymerase activity in Escherichia coli cells infected with bacteriophage T7 resides in a protein complex consisting of the T7 gene 5 protein and E. coli thioredoxin in a 1 to 1 stoichiometry. We have analyzed nine mutant thioredoxins, both in vivo and in vitro, for their ability to interact with the T7 gene 5 protein and stimulate the DNA polymerase and exonuclease activities inherent in gene 5 protein. The efficiency of plating of T7 on E. coli thioredoxin mutants depends strongly on the copy number of the respective mutant thioredoxin allele. Plating efficiencies at a constant copy number correlate well with the affinity of the purified mutant proteins for T7 gene 5 protein. The observed dissociation constant, Kobs, is increased between 5 and several hundredfold at 42 degrees C compared to wild-type thioredoxin. The maximum polymerase activity of the reconstituted gene 5 protein-thioredoxin complex at saturating concentrations of mutant thioredoxins, however, is reduced by less than 20%. Consequently, none of the mutant thioredoxins acts as a competitive inhibitor of wild-type thioredoxin. The active-site disulfide of thioredoxin is not essential for the activities of the gene 5 protein-thioredoxin complex. Both cysteines can be replaced without significantly affecting the maximum polymerase or exonuclease activities. Substitution or alkylation of either cysteine, however, reduces the affinity for gene 5 protein drastically, indicating that the active site is part of the thioredoxin surface involved in the protein-protein interaction.


Subject(s)
Bacterial Proteins/metabolism , DNA-Directed DNA Polymerase/metabolism , Escherichia coli/metabolism , Mutation , T-Phages/metabolism , Thioredoxins/metabolism , Viral Proteins/metabolism , Escherichia coli/genetics , Kinetics , Oxidation-Reduction , T-Phages/genetics , Thioredoxins/genetics , Viral Proteins/genetics
20.
Proc Natl Acad Sci U S A ; 82(11): 3776-80, 1985 Jun.
Article in English | MEDLINE | ID: mdl-16593573

ABSTRACT

A segment of the bacteriophage P1 genome, called the C segment, can be inverted by site-specific recombination; the two different orientations of the invertible segment confer different host ranges to the phage. Inversion is catalyzed by the product of the cin gene which is adjacent to one of the crossover sites flanking the C segment. The Cin-catalyzed recombination can be measured in trans by using tester plasmids in which inversion switches on antibiotic-resistance genes. We show here that an additional sequence, distinct from the two crossover sites, is needed in cis for efficient inversion. This sequence is part of the cin structural gene and stimulates recombination more than 100-fold. We have localized the major enhancer sequence on a 72-base-pair fragment and found its activity to be largely independent of the orientation or position of the sequence with respect to the crossover sites.

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