Unraveling the dataset transcriptomic response of Hydrangea macrophylla stem to mechanical stimulation: De novo assembly and functional annotation.

A crucial attribute of potted ornamental plants is compactness characterized by well branched plants with rather short stems bearing numerous flowers. To gain plant compactness, producers use plant growth regulators (PGRs), in particular growth retardants during culture. However, due to their negative environmental impacts, growth retardants are progressively withdrawn from the market. As a response, eco-friendly alternative methods to chemicals need to be developed. One method consists in mimicking mechanical stimulation (MS) imposed by wind on plants which causes reduction in stem elongation, an increase in stem diameter and an increase in branching, all contributing to plant compactness. So far, few plant species were studied under MS and little is known on molecular response mechanisms to MS. This first transcriptomic data after MS in Hydrangea macrophylla will contribute unravelling how plants respond to mechanical stimuli. RNAseq data were obtained from total mRNA of stems collected 15 min before MS and 1, 3, 24 and 72 h after MS treatment. RNA from non-MS treated plants were used as control. MS treatment consisted in 12 consecutive bendings (i.e. 6 forth and 6 back) applied at 9 a.m. during 1 h and for a single day. From RNAseq data a de novo assembly of the transcriptome was produced and 78,398 transcripts functionally annotated. These transcriptomic data also contribute to a better knowledge of how outdoor crop respond to the increasing frequency of strong harmful winds under climate change.

Recurrent symmetrical bendings cause dwarfing in Hydrangea through spatial molecular regulation of xylem cell walls.

Environmental prejudices progressively lead to the ban of dwarfing molecules in agriculture, and alternatives are urgently required. Mechanical stimulation (MS) is a promising, eco-friendly, and economical technique, but some responses to mechanical stimulation vary from one plant species to another. Additionally, as more frequent and violent wind episodes are forecasted under global climate change, knowledge of plant responses to stimuli mimicking wind sways is decisive for agriculture. However, little is known about plant mechanosensitive responses after long-term, recurrent MS. Here, the effects of 3-week, recurrent, symmetrical bendings (1 or 12 per day) in Hydrangea macrophylla stems are examined. Bendings repressed internode elongation and leaf area development, whereas the diametrical growth of the basal internode is increased. Responses were dose-dependent, and no desensitization was observed during the 3 weeks of treatment. MS was almost as efficient as daminozide for plant dwarfing, and it improved stem robustness. Histological and molecular responses to MS were spatially monitored and were concordant with ongoing primary or secondary growth in the internodes. Our molecular data provide the first knowledge on the molecular paths controlled by mechanical loads in Hydrangea and revealed for the first time the involvement of XYP1 in thigmomorphogenetic responses. MS still had a transcriptional impact 48 h after the last bending session, promoting the expression of XYP1, FLA11, and CAD1 while repressing the expression of EXP3 and XTH33 homologs in accordance with xylogenesis, cell wall thickening, and lignin deposition in the xylem of basal internodes. In upper elongating internodes, repression of XYP1, CAD1, SAMS1, and CDC23 homologs is correlated with ongoing primary, even though stunted, growth. For producers, our findings highlight the potential of MS as a sustainable and economical option for controlling plant compactness in Hydrangea and show valuable reinforcement of stem strength.

Genotypic differences in architectural and physiological responses to water restriction in rose bush.

The shape and, therefore, the architecture of the plant are dependent on genetic and environmental factors such as water supply. The architecture determines the visual quality, a key criterion underlying the decision to purchase an ornamental potted plant. The aim of this study was to analyze genotypic responses of eight rose bush cultivars to alternation of water restriction and re-watering periods, with soil water potential of -20 and -10 kPa respectively. Responses were evaluated at the architectural level through 3D digitalization using six architectural variables and at the physiological level by measuring stomatal conductance, water content, hormones [abscisic acid (ABA), auxin, cytokinins, jasmonic acid, and salicylic acid (SA)], sugars (sucrose, fructose, and glucose), and proline. Highly significant genotype and watering effects were revealed for all the architectural variables measured, as well as genotype × watering interaction, with three distinct genotypic architectural responses to water restriction - weak, moderate and strong - represented by Hw336, 'Baipome' and 'The Fairy,' respectively. The physiological analysis explained, at least in part, the more moderate architectural response of 'Baipome' compared to 'The Fairy,' but not that of Hw336 which is an interspecific hybrid. Such physiological responses in 'Baipome' could be related to: (i) the maintenance of the stimulation of budbreak and photosynthetic activity during water restriction periods due to a higher concentration in conjugated cytokinins (cCK) and to a lower concentration in SA; (ii) a better resumption of budbreak during the re-watering periods due to a lower concentration in ABA during this period. When associated with the six architectural descriptors, cCK, SA and ABA, which explained the genotypic differences in this study, could be used as selection criteria for breeding programs aimed at improving plant shape and tolerance to water restriction.

Light Signaling in Bud Outgrowth and Branching in Plants.

Branching determines the final shape of plants, which influences adaptation, survival and the visual quality of many species. It is an intricate process that includes bud outgrowth and shoot extension, and these in turn respond to environmental cues and light conditions. Light is a powerful environmental factor that impacts multiple processes throughout plant life. The molecular basis of the perception and transduction of the light signal within buds is poorly understood and undoubtedly requires to be further unravelled. This review is based on current knowledge on bud outgrowth-related mechanisms and light-mediated regulation of many physiological processes. It provides an extensive, though not exhaustive, overview of the findings related to this field. In parallel, it points to issues to be addressed in the near future.

Rose bush leaf and internode expansion dynamics: analysis and development of a model capturing interplant variability.

Rose bush architecture, among other factors, such as plant health, determines plant visual quality. The commercial product is the individual plant and interplant variability may be high within a crop. Thus, both mean plant architecture and interplant variability should be studied. Expansion is an important feature of architecture, but it has been little studied at the level of individual organs in rose bushes. We investigated the expansion kinetics of primary shoot organs, to develop a model reproducing the organ expansion of real crops from non-destructive input variables. We took interplant variability in expansion kinetics and the model's ability to simulate this variability into account. Changes in leaflet and internode dimensions over thermal time were recorded for primary shoot expansion, on 83 plants from three crops grown in different climatic conditions and densities. An empirical model was developed, to reproduce organ expansion kinetics for individual plants of a real crop of rose bush primary shoots. Leaflet or internode length was simulated as a logistic function of thermal time. The model was evaluated by cross-validation. We found that differences in leaflet or internode expansion kinetics between phytomer positions and between plants at a given phytomer position were due mostly to large differences in time of organ expansion and expansion rate, rather than differences in expansion duration. Thus, in the model, the parameters linked to expansion duration were predicted by values common to all plants, whereas variability in final size and organ expansion time was captured by input data. The model accurately simulated leaflet and internode expansion for individual plants (RMSEP = 7.3 and 10.2% of final length, respectively). Thus, this study defines the measurements required to simulate expansion and provides the first model simulating organ expansion in rosebush to capture interplant variability.

Mobilisation of nutrients and transport via the xylem sap in a shrub (Ligustrum ovalifolium) during spring growth: N and C compounds and interactions.

In open-field soilless culture there can be great deal of leaching, particularly in rainy springs. Ligneous plants have the capacity to store large quantities of nutrients in perennial organs. Knowledge of the plant's internal nutrient mobilisation during spring to supply growing organs could lead to reduction of fertiliser application. To quantify the fraction of storage mobilisation available for growth of new organs during spring, Ligustrum ovalifolium shrubs were grown for 2 years with or without fertilisation in the second spring. Nitrogen (N) absorption and N and carbon (C) mobilisation from storage were followed during spring growth via the sap quality. A mathematical combination of the sap composition with flow velocity provided the transported quantities of N and C. Nitrogen and C mobilisation towards new shoots took place during all the spring growth from bud break onwards. In unfertilised plants, C was mobilised primarily as sugars (stachyose, mannose and sucrose) and starch. In fertilised plants, the same sugars were transported in the xylem sap, but at lower concentrations. Stachyose concentration was lower in fertilised than in unfertilised plants and decreased during spring growth. Nitrogen was transported in the xylem sap mainly as amino acids in both fertilisation treatments. Glutamine was the predominant form at bud break and during shoot elongation. In fertilised plants, arginine became predominant after shoot elongation, and was related to low C availability. The interactions of N with C are discussed; specifically, insufficient availability of N limits the use of C, more of which is directed to aerial organs by sap flow.