ABSTRACT
The effects of dimethyl sulfoxide (DMSO) were studied in irradiated BALB/c mice to see if DMSO has a stimulatory effect on hematopoietic stem cells. The spleen colony forming technique was employed. DMSO given after a lethal dose of radiation enhanced post-irradiation recovery and survival. The erythroid to granuloid spleen colony ratio was higher in the DMSO treated group compared to the controls. There was some evidence of an increase in the endogenous formation of erythroid colonies after DMSO treatment (0.38%). However, DMSO had no effect on exogenous erythroid colony formation; rather, it inhibited granuloid differentiation.
Subject(s)
Dimethyl Sulfoxide/pharmacology , Hematopoietic Stem Cells/drug effects , Animals , Female , Granulocytes/drug effects , Hematopoietic Stem Cells/radiation effects , Mice , Radiation Dosage , SpleenABSTRACT
In vivo and in vitro survival and response of lymphocytes were studied in 12 lung cancer patients before, during and following radiotherapy, and comparisons were made to data from 20 healthy control subjects. Lymphocyte counts of lung cancer patients prior to radiotherapy did not differ significantly from those of control subjects. Following radiotherapy, lymphocyte counts were significantly reduced. The in vitro survival of lymphocytes from lung cancer patients prior to radiotherapy was lower than that of normal controls. Radiation treatment had no effect on lymphocyte survival in vitro. PHA-stimulated lymphoblast formation in lung cancer patients prior to radiotherapy did not differ from that of normal controls, but decreased significantly during radiotherapy.
Subject(s)
Lung Neoplasms/radiotherapy , Lymphocyte Activation/radiation effects , Lymphocytes/radiation effects , Radiation Injuries/immunology , Cell Survival/radiation effects , Humans , In Vitro Techniques , Leukocyte Count , Lung Neoplasms/blood , Lung Neoplasms/immunology , Male , Middle Aged , Phytohemagglutinins/pharmacology , Time FactorsABSTRACT
Aryl hydrocarbon hydroxylase (AHH) inducibility was studied in cultured lymphocytes from 21 healthy control subjects and from 15 lung cancer patients selected for radiation therapy. AHH inducibility of the patients was measured prior to, during and at the end of radiation therapy. Four of 15 patients had values comparable to the healthy controls. Cellular DNA and protein measurements of cultured lymphocytes were the same for patients and healthy controls. There was no significant difference in the percentage of lymphoblast formation and percentage of cell survival between the two groups. Radiation therapy reduces the number of lymphocytes in vivo and the amount of lymphoblast formation in vitro. AHH inducibulity is signifcantly lowered by radiation in the patients who had very high inducibility at pre-treatment level. DNA and protein contents of cultured lymphocytes did not change during radiation therapy.
Subject(s)
Aryl Hydrocarbon Hydroxylases/biosynthesis , Lung Neoplasms/enzymology , Lymphocytes/enzymology , Aged , Cells, Cultured , DNA/analysis , DNA, Neoplasm/analysis , Enzyme Induction/radiation effects , Humans , Lung Neoplasms/immunology , Lung Neoplasms/radiotherapy , Lymphocyte Activation/radiation effects , Male , Middle Aged , Neoplasm Proteins/analysis , Proteins/analysisABSTRACT
Aryl hydrocarbon hydroxylase (AHH) inducibility and lymphoblast formation were studied in lymphocytes from healthy control subjects and from lung cancer patients undergoing radiotherapy. The relationship between AHH inducibility and percentage lymphoblasts was statistically significant only for the pre-treatment patients (r = 0.598; p less than 0.05). In the control group and in patients undergoing radiotherapy the correlation between AHH inducibility and lymphoblast formation was positive but statistically it was not significant. Our data do not suggest a linear relationship between AHH inducibility and lymphoblast formation.
Subject(s)
Aryl Hydrocarbon Hydroxylases/biosynthesis , Lung Neoplasms/enzymology , Lymphocyte Activation , Enzyme Induction , Humans , Lung Neoplasms/immunology , Lung Neoplasms/radiotherapy , Male , Middle AgedABSTRACT
A group of patients treated with radiation therapy for bronchogenic carcinoma were evaluated with serial pulmonary function testing. The patients were treated with a split-course technique with 60-Cobalt. A total of 30 patients with unresectable carcinoma have been studied. Measurements of vital capacity (V.C.) and forced expiratory volume at one second (F.E.V.1) were made prior to therapy; at the beginning and end of the split; upon completion of therapy; and thereafter at 1, 2, and 6 months post therapy. The percentage changes in V.C. and F.E.V.1 from pretreatment values are plotted against time. A trend to improve throughout the treatment course is suggested by these results. Twelve patients have been studied at six-month follow-up: eight of the patients showed less than a 10% reduction from pretreatment V.C. and F.E.V.1 and had no respiratory impairment; of the other four patients, three had evidence of progression of disease in the thorax.
Subject(s)
Carcinoma, Bronchogenic/radiotherapy , Forced Expiratory Volume , Lung Neoplasms/radiotherapy , Vital Capacity/radiation effects , Carcinoma, Bronchogenic/physiopathology , Cobalt Radioisotopes/therapeutic use , Female , Humans , Lung Neoplasms/physiopathology , Male , Radioisotope Teletherapy , Radiotherapy DosageSubject(s)
Bile Duct Neoplasms/radiotherapy , Cholestasis/radiotherapy , Laryngeal Neoplasms/radiotherapy , Liver Neoplasms/radiotherapy , Lymphoma/radiotherapy , Adenocarcinoma/radiotherapy , Bile Duct Neoplasms/complications , Cholestasis/etiology , Cobalt Radioisotopes , Epiglottis , Humans , Laryngeal Neoplasms/complications , Liver Neoplasms/complications , Lymphoma/complications , Male , Middle Aged , Neoplasm Metastasis , Palliative Care , Radioisotope Teletherapy , Radiotherapy, High-EnergyABSTRACT
A small number of patients with limited stage C carcinoma of the prostate have been treated with combined interstitial and external beam radiotherapy with gratifying results. Whereas more patients will have to be followed for at least 10 to 15 years to completely evaluate this modality of treatment, the results continue to encourage us in its use.
Subject(s)
Carcinoma/radiotherapy , Prostatic Neoplasms/radiotherapy , Biopsy , Carcinoma/pathology , Cystitis/etiology , Erectile Dysfunction/etiology , Humans , Male , Proctitis/etiology , Prostatic Neoplasms/pathology , Radiotherapy/adverse effects , Radiotherapy DosageABSTRACT
A small number of patients with limited stage C carcinoma of the prostate have been treated with combined interstitial and external beam radiotherapy with gratifying results. Whereas more patients will have to be followed for at least 10 to 15 years to completely evaluate this modality of treatment, the results continue to encourage us in its use.
