Biomechanical Analysis of Porcine Cartilage Elasticity.

Grafting of tissue-engineered cartilage to joints with osteoarthritis has the potential to supersede arthroplasty as the standard of care. However, in order to support the development of functional tissue engineering methods, the subfailure biomechanics of the individual cartilage types that comprise joints must be determined. Current methods for analyzing tissues are based on imaging and are therefore unable to profile the strain dependence of mechanical behaviors within different cartilage types. Recently, an analysis technique based on Optical Fiber Polarimetric Elastography (OFPE) has overcome these challenges. OFPE has been used to characterize the different mechanical behaviors of a range of unprocessed biomaterials and tissues. In the present work, this technique is used to characterize the biomechanics of both articular cartilage and meniscal fibrocartilage within a porcine knee. OFPE testing of the tissue is conducted over a range of physiological loading and unloading values. These results demonstrate the distinctive mechanics of each cartilage type. Due to their different locations within the knee, each cartilage type exhibits distinctly unique biomechanical behavior. Based on the results of OFPE, we correlate the specific buckling, delamination, and bridging events to maxima and minima along the loading and unloading curves. This provides unprecedented detail with regard to the subfailure biomechanics. This information is integral to the design of the next generation of tissue-engineered constructs. Therefore, OFPE will be used across multiple disciplines to rapidly determine the mechanical behavior of tissue-engineered constructs to support functional tissue engineering efforts.

Role of extracellular matrix in the biomechanical behavior of pancreatic tissue.

Correlating the biomechanical properties of tissue with its function is an emerging area of research with potential impact in diagnostics, therapeutics, and prognostics. A critical stepping-stone in developing structure-function models is creating methods that can correlate the tissue structure with its mechanical behavior. As an initial step in addressing this challenge, we have characterized the mechanical behavior of unprocessed pancreatic tissue using optical fiber polarimetric elastography. To correlate the observed behavior to physiologically relevant structural features, a series of architectures are designed and fabricated using 3D printing. The mechanical response of the 3D printed elastomeric structures is analyzed using compressive testing and modeled using finite element analysis. The biomechanical behavior and buckling point of the 3D printed structures is used to create a calibration curve to understand the measured response of the resected pancreatic tissue. Based on the modeling and biomimetic results, the biomechanical behavior of pancreatic tissue is likely due to the collagen IV network.

Characterization of the mechanical properties of resected porcine organ tissue using optical fiber photoelastic polarimetry.

Characterizing the mechanical behavior of living tissue presents an interesting challenge because the elasticity varies by eight orders of magnitude, from 50Pa to 5GPa. In the present work, a non-destructive optical fiber photoelastic polarimetry system is used to analyze the mechanical properties of resected samples from porcine liver, kidney, and pancreas. Using a quasi-linear viscoelastic fit, the elastic modulus values of the different organ systems are determined. They are in agreement with previous work. In addition, a histological assessment of compressed and uncompressed tissues confirms that the tissue is not damaged during testing.

On-chip asymmetric microcavity optomechanics.

High quality factor (Q) optical resonators have enabled rapid growth in the field of cavity-enhanced, radiation pressure-induced optomechanics. However, because research has focused on axisymmetric devices, the observed regenerative excited mechanical modes are similar. In the present work, a strategy for fabricating high-Q whispering gallery mode microcavities with varying degrees of asymmetry is developed and demonstrated. Due to the combination of high optical Q and asymmetric device design, two previously unobserved modes, the asymmetric cantilever and asymmetric crown mode, are demonstrated with sub-mW thresholds for onset of oscillations. The experimental results are in good agreement with computational modeling predictions.