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1.
Chem Sci ; 15(15): 5723-5729, 2024 Apr 17.
Article in English | MEDLINE | ID: mdl-38638209

ABSTRACT

Previously, we have shown that native ambient mass spectrometry imaging allows the spatial mapping of folded proteins and their complexes in thin tissue sections. Subsequent top-down native ambient mass spectrometry of adjacent tissue section enables protein identification. The challenges associated with protein identification by this approach are (i) the low abundance of proteins in tissue and associated long data acquisition timescales and (ii) irregular spatial distributions which hamper targeted sampling of the relevant tissue location. Here, we demonstrate that these challenges may be overcome through integration of laser capture microdissection in the workflow. We show identification of intact protein assemblies in rat liver tissue and apply the approach to identification of proteins in the granular layer of rat cerebellum.

2.
J Am Chem Soc ; 145(29): 15658-15662, 2023 07 26.
Article in English | MEDLINE | ID: mdl-37459360

ABSTRACT

Native ambient mass spectrometry enables the in situ analysis of proteins and their complexes directly from tissue, providing both structural and spatial information. Until recently, the approach was applied exclusively to the analysis of soluble proteins; however, there is a drive for new techniques that enable analysis of membrane proteins. Here we demonstrate native ambient mass spectrometry of membrane proteins, including ß-barrel and α-helical (single and multipass) integral membrane proteins and membrane-associated proteins incorporating lipid anchors, by integration of a simple washing protocol to remove soluble proteins. Mass spectrometry imaging revealed that washing did not disrupt the spatial distributions of the membrane and membrane-associated proteins. Some delocalization of the remaining soluble proteins was observed.


Subject(s)
Membrane Proteins , Membrane Proteins/chemistry , Mass Spectrometry/methods
3.
Methods Mol Biol ; 2688: 55-62, 2023.
Article in English | MEDLINE | ID: mdl-37410283

ABSTRACT

Liquid extraction surface analysis (LESA) is an ambient surface sampling technique that can be coupled with mass spectrometry (MS) to analyze analytes directly from biological substrates such as tissue sections. LESA MS involves liquid microjunction sampling of a substrate by use of a discrete volume of solvent followed by nano-electrospray ionization. As the technique makes use of electrospray ionization, it lends itself to the analysis of intact proteins. Here, we describe the use of LESA MS to analyze and image the distribution of intact denatured proteins from thin fresh frozen tissue sections.


Subject(s)
Proteins , Spectrometry, Mass, Electrospray Ionization , Mass Spectrometry/methods , Proteins/chemistry , Spectrometry, Mass, Electrospray Ionization/methods
4.
Angew Chem Int Ed Engl ; 61(36): e202202075, 2022 09 05.
Article in English | MEDLINE | ID: mdl-35830332

ABSTRACT

Here, we demonstrate detection by mass spectrometry of an intact protein-drug complex directly from liver tissue from rats that had been orally dosed with the drug. The protein-drug complex comprised fatty acid binding protein 1, FABP1, non-covalently bound to the small molecule therapeutic bezafibrate. Moreover, we demonstrate spatial mapping of the [FABP1+bezafibrate] complex across a thin section of liver by targeted mass spectrometry imaging. This work is the first demonstration of in situ mass spectrometry analysis of a non-covalent protein-drug complex formed in vivo and has implications for early stage drug discovery by providing a route to target-drug characterization directly from the physiological environment.


Subject(s)
Bezafibrate , Liver , Animals , Bezafibrate/analysis , Bezafibrate/metabolism , Diagnostic Imaging , Drug Discovery , Liver/metabolism , Mass Spectrometry , Rats
5.
Anal Chem ; 94(14): 5608-5614, 2022 04 12.
Article in English | MEDLINE | ID: mdl-35358391

ABSTRACT

Untargeted label-free interrogation of proteins in their functional form directly from their physiological environment promises to transform life sciences research by providing unprecedented insight into their transient interactions with other biomolecules and xenobiotics. Native ambient mass spectrometry (NAMS) shows great potential for the structural analysis of endogenous protein assemblies directly from tissues; however, to date, this has been limited to assemblies of low molecular weight (<20 kDa) or very high abundance (hemoglobin tetramer in blood vessels, RidA homotrimer in kidney cortex tissues). The present work constitutes a step change for NAMS of protein assemblies: we demonstrate the detection and identification of a range of intact endogenous protein assemblies with various stoichiometries (dimer, trimer, and tetramer) from a range of tissue types (brain, kidney, liver) by the use of multiple NAMS techniques. Crucially, we demonstrate a greater than twofold increase in accessible molecular weight (up to 145 kDa). In addition, spatial distributions of protein assemblies up to 94 kDa were mapped in brain and kidney by nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging.


Subject(s)
Scrapie , Spectrometry, Mass, Electrospray Ionization , Animals , Brain/metabolism , Kidney/metabolism , Proteins/metabolism , Sheep , Spectrometry, Mass, Electrospray Ionization/methods
6.
Angew Chem Weinheim Bergstr Ger ; 134(36): e202202075, 2022 Sep 05.
Article in English | MEDLINE | ID: mdl-38505542

ABSTRACT

Here, we demonstrate detection by mass spectrometry of an intact protein-drug complex directly from liver tissue from rats that had been orally dosed with the drug. The protein-drug complex comprised fatty acid binding protein 1, FABP1, non-covalently bound to the small molecule therapeutic bezafibrate. Moreover, we demonstrate spatial mapping of the [FABP1+bezafibrate] complex across a thin section of liver by targeted mass spectrometry imaging. This work is the first demonstration of in situ mass spectrometry analysis of a non-covalent protein-drug complex formed in vivo and has implications for early stage drug discovery by providing a route to target-drug characterization directly from the physiological environment.

7.
Anal Chem ; 92(4): 2885-2890, 2020 02 18.
Article in English | MEDLINE | ID: mdl-31967787

ABSTRACT

The benefits of high field asymmetric waveform ion mobility spectrometry (FAIMS) for mass spectrometry imaging of intact proteins in thin tissue sections have been demonstrated previously. In those works, a planar FAIMS device coupled with a Thermo Elite mass spectrometer was employed. Here, we have evaluated a newly introduced cylindrical FAIMS device (the FAIMS Pro) coupled with a Thermo Fusion Lumos mass spectrometer for liquid extraction surface analysis mass spectrometry imaging of intact proteins in thin tissue sections from rat testes, kidney, and brain. The method makes use of multiple FAIMS compensation values at each location (pixel) of the imaging array. A total of 975 nonredundant protein species were detected in the testes imaging dataset, 981 in the kidney dataset, and 249 in the brain dataset. These numbers represent a 7-fold (brain) and over 10-fold (testes, kidney) improvement on the numbers of proteins previously detected in LESA FAIMS imaging, and a 10-fold to over 20-fold improvement on the numbers detected without FAIMS on this higher performance mass spectrometer, approaching the same order of magnitude as those obtained in top-down proteomics of cell lines. Nevertheless, high throughput identification within the LESA FAIMS imaging workflow remains a challenge.


Subject(s)
Proteins/analysis , Animals , Brain , Cell Line , Ion Mobility Spectrometry , Kidney/chemistry , Male , Mass Spectrometry , Proteomics , Rats , Rats, Wistar , Testis/chemistry
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