ABSTRACT
Head and neck primary soft tissue sarcoma is a rare adult connective tissue malignant tumor derived from mesenchymal tissue, which can occur in the paranasal sinuses, throat or neck space.The clinical manifestations are local spread masses in the head and neck or difficulty breathing, swallowing, etc al. MRI and enhanced CT examination are the most commonly used to diagnose such diseases. Pathological diagnosis requires immunohistochemistry combined with FISH to detect MDM2 and CDK4. In this reportï¼two cases of primary soft tissue sarcoma were reported,one is parotid high-differentiated liposarcoma and the other is laryngeal dedifferentiated leiomyosarcoma, introducing the characteristics diagnosis and treatment, and reviewing the relevant literature.
Subject(s)
Head and Neck Neoplasms , Liposarcoma , Sarcoma , Soft Tissue Neoplasms , Humans , Liposarcoma/diagnosis , Proto-Oncogene Proteins c-mdm2 , Sarcoma/diagnosis , Soft Tissue Neoplasms/diagnosis , Head and Neck Neoplasms/diagnosisABSTRACT
Laryngeal schwannoma is a rare clinical benign neurogenic tumor that originated in the larynx. Large laryngeal schwannoma can cause foreign body sensation in the pharynx and swallowing obstruction. Clinical symptoms includehoarseness and dyspnea. The enhanced CT scan illustrates that the localized mass is isolated, with clear boundary with the surrounding tissues in the larynx. A 68-year-old woman presented with a swallowing discorder,endoscopy demonstrated a smooth mass of the left aryepiglottic fold.The patient then underwent endoscopy surgey supported by the low temperature plasma instead of an open incision.
Subject(s)
Laryngeal Neoplasms , Larynx , Neurilemmoma , Aged , Endoscopy , Female , Humans , Laryngeal Neoplasms/surgery , Neurilemmoma/diagnostic imaging , Neurilemmoma/surgery , TemperatureABSTRACT
OBJECTIVE: To study the relationship between the radiotherapy resistance and autophagy. To provide a theoretiacal basis for drugs that regulate autophagy to improve radiotherapy sensitivity. METHOD: Flow cytometry (FCM) was performed to analyze the distribution of the cell cycle of CNE2 and CNE2/DDP cells under the action of X radiation. The expression of autopagy-specific gene Beclin1 and microtubule-associated protein light chain 3ß (MAPLC3ß) in CNE2 and CNE2/DDP cells was determined by real time PCR and Immumofluorescence staining. RESULT: CNE2/DDP and their parental CNE2 cells produced the G2-M phase arrest under the action of X radiation. With the radiation dose increasing,The cells which in the G2-M phase were more and more (P<0. 05). The G2-M phase arrest in CNE2/DDP cells was more obvious than in CNE2 cells (P<0. 05). The expression of Beclin1 and MAPLC3ß in CNE2 and CNE2/DDP cells increased under the action of X radiation. What's more, the raise was more and more obvious with the increase of the irradiation dose(P<0. 05). The expression levels of Beclin1 and MAPLC3ß in CNE2/DDP was lower than that in CNE2 cells (P<0. 05). CONCLUSION: Autophagic cell death may be the one manner of death in nasopharyngeal carcinoma CNE2 and CNE2/DDP cells under the action of X radiation. The radiation resistance of CNE2/DDP cells may be related to the low expression of autophagy-related genes.
