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Repeated implantation failure (RIF) is one of the most prominent problems in the field of assisted reproduction. Neu5Gc on the surface of decidual macrophages (dMΦ) leads to different activation patterns of dMΦ, which affects embryo implantation and development. Cmah-/- (Neu5Gc-deficient) mice induced to produce anti-Neu5Gc antibodies in vivo were given a special diet rich in Neu5Gc and their fertility was monitored. The long-term diet rich in Neu5Gc induced the decrease of endometrial receptivity of female mice. The pregnancy rate of female mice fed the normal diet was 63.6% (n = 11) and the average number of embryos was 9.571 ± 1.272, while the pregnancy rate of female mice fed the diet rich in Neu5Gc was 36.4% (n = 11) and the average number of embryos in pregnant mice was 5.750 ± 3.304. The intake of Neu5Gc and the production of anti-Neu5Gc antibody led to M1 polarization of endometrial dMΦ and abnormal embryo implantation.
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Embryo Implantation , Macrophages , Pregnancy , Animals , Female , MiceABSTRACT
Objective:To explore the impact of intervention based on the SMG health management model on self-efficacy and medical coping styles of patients with moderate to severe stroke.Methods:In this experimental study, 106 stroke patients who visited the rehabilitation medicine department of the Second Affiliated Hospital of Xinjiang Medical University from April 2022 to August 2022 were selected by drawing lots and randomly divided into a control group (53 cases) and an intervention group (53 cases). The control group received routine health management after early stroke rehabilitation, while the intervention group was included in the SMG health management model intervention for 8 weeks based on the control group. The Medical Coping Style Questionnaire and Stroke Self-efficacy Questionnaire were used to compare the medical coping style and self-efficacy of the two groups and compared the results.Results:The total scores of coping style and self-efficacy in the intervention group were (16.42 ± 2.79) and (59.86 ± 13.84), which were higher than those in the control group (14.80 ± 1.70) and (35.96 ± 13.92) ( t = 3.50, 8.61, both P<0.05). The scores of avoidance and surrender coping styles were (13.28 ± 1.60) and (7.16 ± 2.10), which were lower than the control group′s (14.66 ± 0.77) and (8.26 ± 1.01) scores ( t = -5.48, -3.34, both P<0.05). Conclusions:Integrating the SMG health management model intervention into the early rehabilitation nursing process of patients with moderate to severe stroke can help improve patients′ self-efficacy, transform positive disease coping styles, actively cooperate with medical staff for rehabilitation training to improve disease prognosis.
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Objective To investigate the epidemiological and clinical features of brucellosis in Foshan.Methods The epidemiological history,clinical manifestations,laboratory tests,treatment and outcomes of 41 patients with brucellosis from 2013 to 2016 in the First People's Hospital of Foshan were retrospectively analyzed.Results Brucellosis onsets occurred mainly from February to June [85.4％ (35/41)],and 58.5％ (24/41) of them had positive epidemic history.Irregular fever,rachialgia/arthralgia,fatigue and hepatosplenomegaly occurred in 29 (70.7％),20 (48.8％),19 (46.3％) and 16 (39.0％) of the patients,respectively.The C-reactive protein (hs-CRP) of patients infected with Brucella only was lower than that in patients infected also with other bacteria (26.72 vs 50.87 mg/L,Z =-2.300,P ＜ 0.05),but no significant difference of white blood cell counts (5.77 × 109/L vs 5.83 × 109/L),neutrophil (3.50 × 109/L vs 3.84 × 109/L) and procalcitonin (PCT,0.10 vs 0.14 μg/L) between the two groups were observed.The patients with positive epidemic history had lower white blood cell,neutrophil and monocyte counts than those who did not had epidemic history (4.73 × 109/Lvs 7.28 × 109/L,2.73 × 109/L vs 4.79 × 109/L,and 0.36 × 109/L vs 0.64 × 109/L;F =9.486,10.130,9.785,P ＜ 0.05).And no significant difference of lymphocyte counts,hs-CRP and PCT between the two groups were observed (1.57 × 109/L vs 1.73 × 109/L,29.30 vs 35.76 mg/L,and 0.15 vs 0.09 μg/L;P ＞ 0.05).All the cases were infected by Brucella melitensis,and 33 of them were sensitive to general antibiotics in vitro.There were 40 cases discharged after treatment,and 34 cases still needed to increase antibiotic treatment courses.Most patients had good outcomes.Conclusions In Foshan,patients with irregular fever and rachialgia or arthralgia,and no significantly increased inflammation index,should be aware of brucellosis.We should strengthen the screening of brucellosis in Foshan.
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Objective To investigate the correlation of self perceived burden, family cohesion and adaptability in advanced cancer patients. Methods The Chinese version self perceived burden scale (SPBS), Chinese version of family cohesion and Adaptability Scale (FACES-CV) were used to investigate 139 cases of patients with advanced cancer, and to analyze their correlation with family cohesion and adaptability compared with the norm. Results Patients' self perceived burden score (35.83 ± 5.59) points in the moderate level. The 3 dimensions of the item score from high to low was economic burden (4.12 ± 0.86), body burden (3.63 ± 0.53), emotional burden (3.49 ± 0.63); survey of patients with family cohesion and adaptability, intimacy score (67.96 ± 9.35) points, the ideal intimacy score (83.68 ± 6.05), satisfaction score (15.71 ± 9.39) points, were higher than the national norm, the actual adaptability score (48.00 ± 6.92) and ideal adaptability score (56.47 ± 4.99) points lower than the national norm, the difference was statistically significant (P<0.01 or 0.05). Patients' self perceived burden score and intimacy was negatively correlated (r=-0.186, P<0.05), and positively correlated with intimacy satisfaction (r=0.175, P<0.05). Regression analysis, gender (B=0.236, P<0.01), family medical burden (B=0.183, P<0.05), actual family cohesion (B=-0.111, P<0.05) was the influencing factors of patients' self perceived burden. Conclusions The burden of self perception in advanced cancer patients is closely related to family cohesion and adaptability. It is suggested that the medical staff should pay attention to the family psychological intervention and provide emotional connection for the patients and their families, so as to reduce the negative emotion of the patients with advanced cancer.
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Objective To explore the correlations of between expectations and anxiety and depression of cervical cancer patients. Method Herth hope index,self-rating anxiety scale(SAS)and self rating depression scale(SDS)and self-made general information questionnaire were used to investigate the levels of hope,anxiety and depression as well as the correlations between them.Results The overall level of hope in cervical cancer patients was(36.89±4.21)in a moderate to high level. The scores on hope level and keeping intimate relationships with other in the group of patients,aged above 50 years,married,with high school education or above and monthly income of 1,500 yuan were significantly higher than those of the other group patients.The incidences of anxiety and depression were 47.83%and 52.17%, respectively. The general hope level and its three dimensions was negatively related to the anxiety and depression(P<0.01).Conclusions The hope of cervical cancer patients are at moderate to high level and are negatively correlated between anxiety and depression.Improving the hope level of cervical cancer patients can effectively improve patients bad feelings.
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Objective To understand the structures and functions of enterovirus 71 (EV71)VP1 gene and its encoded protein using bioinformatics method, so as to direct studies of its biological function. Methods VP1 gene and its encoded protein of EV71 2008-GZCH07 strain and other representative EV71 strains were analyzed by online analysis at bioinformatics websites and software packages. Multi-sequence homological alignment and phylogenetic analysis were performed.Physicochemical characteristics, secondary structure, homology modeling of tertiary structure,enzymological characteristics, antigenic epitope of VP1 gene encoded protein were predicted. Results The homology of EV71 2008-GZCH07 strain was highest (97% and 98%) with ZJ001 strain, and lowest with human coxsackievirus A16. The homology of EV71 2008-GZCH07 strain and EV71 types A,B,C was 86%-98%. Phylogenetic analysis demonstrated that 2008-GZCH07 stain was close to ZJ001 and BJ08-Z025-5 stains, which belonged to C4 subtype. In VP1 encoded proteins of EV71 types A,B,C, the relationship between 2008-GZCH07 and EV71-B, EV71-C was closer than EV71-A.The whole length of VP1 gene was 510 bp, with open reading frame (ORF) located at 116-510 bp region,and it encoded 132 amino acids with isoelectric point of 4.39.The protein was rich of a-helix and random coilon without transmembrane regions, and contained 5 high hydrophobic regions and belonged to extracellular protein. The homology modeling of tertiary structure showed that the region was on the surface of protein and formed a binding loop. There was 5 antigen epitopes. And 7 key catalytic sites were located at or close to the loop. Conclusions EV71-VP1 encoded protein contains many phosphorylation sites, with many biological function sites and antigenic epitope regions, which might be a potential target antigen for immunodiagnosis, anti-schistosome drug and vaccine development, and would be basis of further study of diagnosis, treatment and prevention of EV71 infection.
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In this paper, a newly-designed device is introduced, which uses non-dispersive infrared absorption technique to acquire anesthesia gas concentration signal and to perform side-stream measurement in the air way. The microcontroller is adopted to implement data acquisition, data processing and then to transmit the data to the PC screen, thus the real-time continuous invasive concentration monitoring of the anesthesia gas in the patient's respiratory air can be realized during a surgical operation.
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The plateau personal physiological parameters monitor can be applied to casualty rescue at peacetime and wartime and trainings of troops in the plateau. With the function of telemering, the monitor can give realtime data of O2Sat, pulse and temperature when the subject is in an environment such as the plateau or the one of high and cold.
