CD4+CD25+CD127low/- regulatory T cells and related cytokines in patients with psoriasis vulgaris / 实用医学杂志

Objective To explore the role of CD4+CD25+CD127low/-regulatory T cells (Tregs) in the pathogenesis of psoriasis vulgaris(PV). Methods Flow cytometry analysis was used to detect the amount of Tregs in peripheral blood and ELISA to test the levels of IL-10 and TGF-β1 in blood serum; the suppressive function of Tregs on autologous CD4+CD25-T cells was determined by MTT method. Results No significant difference was found in the proportion of Tregs in PV patients and healthy controls(P>0.05). There was a diminished suppression of Tregs from patients on autologous CD4+CD25- responder T cell proliferation in PV patients when compared with that in controls (P < 0.01). The serum level of IL-10 in patients was lower than that in controls (P < 0.01) while that of TGF-β1 in PV patients was significantly higher than that in controls(P < 0.01). Conclusion Abnormal function of Tregs and low secretion of IL-10 in PV patients might be related to the pathogenesis of psoriasis.

Elevated Local and Serum CX3CL1(Fractalkine) Expression and Its Association with Disease Severity in Patients with Psoriasis.

BACKGROUND AND OBJECTIVE: Psoriasis is a chronic immune and inflammatory skin disease. Fractalkine (FKN, CX3CL1), a membrane-bound CX3C chemokine, has been identified to play an important role in the pathogenesis of psoriasis. However, whether it is elevated in the tissue or peripheral blood of patients with psoriasis and is associated with disease severity is unclear. This study was carried out to explore local and serum FKN expression in patients with psoriasis and investigate the relationship with the disease severity. METHODS: Serum samples were collected from 47 plaque psoriasis patients and 49 healthy individuals. Serum FKN levels were measured by an enzymelinked immunosorbent assay. The PASI scores of patients with psoriasis and their correlation with serum FKN levels were evaluated. 16 cases of local skin tissues were collected from psoriasis patients and controls who underwent traumatic incidents needing autologous skin grafting, respectively. FKN protein and mRNA expression were examined using Western blotting and RT-PCR, respectively. RESULTS: Local and serum expression of FKN were significantly higher than the expression in the controls (P<0.05), serum FKN levels were positively associated with Psoriasis Area and Severity Index (PASI) scores, (P<0.001) and levels of IL-22 (P<0.001) and IFN-γ (P<0.001). CONCLUSION: These results suggest that FKN may play an important role in the pathogenesis of psoriasis and represent a reliable biomarker to reflect disease severity. Therapeutic interventions that target FKN in psoriasis deserve further study.

Expression and significance of autophagic gene Beclin 1 and MAP1 LC3 protein in cutaneous malignant melanoma / 临床与实验病理学杂志

Purpose To detect the expression of autophagic genes Beclin 1 and MAP1LC3 in cutaneous malignant melanoma and to ex-plore the relationship between autophagia and malignant melanoma. Methods 85 cases of speicmens including normal skin tissue, in-tradermal nevi, radial growth phase melanomas, vertical growth phase melanomas, and metastatic melanoma were collected, and the protein expression of Beclin 1 and MAP1LC3 were evaluated by immunohistochemistry of SP methods. Results The Beclin 1 and MAP1LC3 expression were pretended to be 100% in normal skin tissue, and they were declined to 85% and 95% in intradermal nevi, 58% and 50% in radial growth phase melanomas, 49. 5% and 44. 4% in vertical growth phase melanomas, both of 17% in melanoma metastases (P<0. 05). Conclusion Beclin 1 and MAP1LC3 autophagic gene expression were significantly decreased with tumor pro-gression, as well as was correlated with conventional histopathologic prognostic factors.

Effect of Artesunate on Experimental Immune Myositis Animal Model / 中华皮肤科杂志

Objective To investigate the effect of Artesunate (Art) on experimental immune myositis (EIM) animal model. Methods Guinea pigs were immunized with rabbit muscle homogenate in Freund′s adjuvant, and a generalized myositis fundamentally similar to human polymyositis was established. These animals were divided into two groups: the control group (injected intraperitoneally with normal saline everyday) and the treatment group (injected intraperitoneally with Art everyday). Results The serum levels of CPK, AST and LDH were significantly lower in the treatment group than those in the control group. EMG demonstrated that the treatment group kept improving along with the elapse of time. Histological findings revealed that the damage of muscle in the treatment group was less severe than that of the control group. Conclusion Art is effective in therapy of EIM guinea pigs. The result of this study provides a reliable evidence that Art might be used for the treatment of PM/DM and other immune diseases.