ABSTRACT
The Subhuman Primate Pregnancy Tube Test (SHPT test) was useful for early pregnancy diagnosis in cynomolgus monkeys (Macaca fascicularis). Test results were correct in 86.5% of series of four successive urine samples. SHPT test results were not confirmed in 13.5% of series, comprising two (false) negative series in pregnant animals and 13 (false) positive series in nonpregnant animals. In ten of 13 false-positive series, a positive-negative sequence was observed in successive test results, presumably due to a decline in chorionic gonadotropin levels. In all animals with a false-positive SHPT test, early embryonic mortality was most probable, since all showed normal cycling activity immediately afterwards. Postmating bleeding records were not reliable for pregnancy diagnosis. A practical approach for the use of the SHPT test in the individual breeding of monkeys is given.
Subject(s)
Macaca fascicularis/physiology , Macaca/physiology , Pregnancy Tests, Immunologic/veterinary , Animals , Chorionic Gonadotropin/urine , Embryo Implantation , False Negative Reactions , False Positive Reactions , Female , Hemagglutination Inhibition Tests/veterinary , Menstruation , PregnancyABSTRACT
The addition of 100 g/l NaCl and 200 mg/l nitrofurantoin to 24-h urine samples destined for determination of LH and FSH with haemagglutination tests, proved to be an adequate preservative in comparison to chilling.
Subject(s)
Follicle Stimulating Hormone/urine , Luteinizing Hormone/urine , Adult , Female , Hemagglutination Inhibition Tests , Humans , Middle AgedABSTRACT
1. A study of the precision of clinical estriol- and total estrogen determinations in late pregnancy urine was carried out in collaboration with 26 clinical laboratories in the Netherlands and one laboratory in Suriname. 2. Ten urine samples were circulated twice with an interval of 2 weeks. 3. It was shown, that repeated analysis of the same sample in the same clinical laboratory in different assays can yield differences in results up to 40%. This interassay variation can be regarded as the main source of uncertainty of results of clinical estriol and total estrogen determinations. 4. The quantitative differences between results of total estrogen methods and a gas chromatographic method, which measures only estriol, were shown to be caused primarily by the lower recovery of the glucuronide of estriol (both native and added) in the latter method. 5. As expected, methods based on the principle of Ittrich (1960), Acta Endocrinol. 35, 34-48) proved to be more susceptible to the disturbing influence of glucose than a gas chromatographic method that measured estriol specifically. 6. Various recommendations to improve the precision of clinical estrogen determinations in pregnancy urine resulted from this study.
Subject(s)
Estriol/urine , Estrogens/urine , Pregnancy Trimester, Third , Evaluation Studies as Topic , Female , Humans , Methods , PregnancyABSTRACT
In the use of anabolic agents, the most pronounced effect on growth is caused by estrogens. For this reason primarily attention will be given to the methods of detection of estrogen administration to fattening animals. The detection methods can mainly be divided in histological, biological, chemical, and immunological determinations and these will be briefly discussed in the light of the present situation in many countries, where the use of anabolic agents is prohibited. From the point of view of control, this prohibition is much easier to handle than a situation in which the application of some specified products is permitted. The possibilities and limitations of control, when certain anabolic agents are permitted for use, will be discussed and evaluated. The conclusion is drawn that in this latter case a sufficient control is very difficult if at all possible considering the methods of control available at the time.
