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1.
Pillars article: cell sorting: automated separation of Mammalian cells as a function of intracellular fluorescence. Science. 1969. 166: 747-749.
Hulett, H R; Bonner, W A; Barrett, Janet; Herzenberg, Leonard A.
J Immunol ; 193(5): 2045-7, 2014 Sep 01.
Article in English | MEDLINE | ID: mdl-25128549

Subject(s)
Antibody-Producing Cells/cytology , Spleen/cytology , Animals , Antibody-Producing Cells/immunology , CHO Cells , Cricetinae , Cricetulus , Flow Cytometry/history , Flow Cytometry/instrumentation , Flow Cytometry/methods , History, 20th Century , Mice , Sheep , Spleen/immunology
2.
Development and application of a rapid cell sorter.
Hulett, H R; Bonner, W A; Sweet, R G; Herzenberg, L A.
Clin Chem ; 19(8): 813-6, 1973 Aug.
Article in English | MEDLINE | ID: mdl-4200173

Subject(s)
Cytological Techniques/instrumentation , Animals , B-Lymphocytes/immunology , Cell Separation , Chromosome Aberrations/diagnosis , Chromosome Disorders , Concanavalin A , Female , Fluoresceins , Fluorescent Antibody Technique , Goats/immunology , Humans , Iodine Isotopes , Lasers , Lymphocytes/immunology , Maternal-Fetal Exchange , Methods , Mice/immunology , Pregnancy , Prenatal Diagnosis , Quaternary Ammonium Compounds , Rh-Hr Blood-Group System/analysis , Scattering, Radiation , Serum Albumin , Spectrometry, Fluorescence , T-Lymphocytes/immunology , Xanthenes
3.
Fluorescence activated cell sorting.
Bonner, W A; Hulett, H R; Sweet, R G; Herzenberg, L A.
Rev Sci Instrum ; 43(3): 404-9, 1972 Mar.
Article in English | MEDLINE | ID: mdl-5013444

Subject(s)
Cell Biology/instrumentation , Erythrocytes/cytology , Fluorescence , Animals , Cells , Chickens , Photochemistry/instrumentation
4.
An improved cell volume analyzer.
Merrill, J T; Veizades, N; Hulett, H R; Wolf, P L; Herzenberg, L A.
Rev Sci Instrum ; 42(8): 1157-63, 1971 Aug.
Article in English | MEDLINE | ID: mdl-5125114

Subject(s)
Blood Cells , Blood Cell Count , Humans , Methods , Reticulocytes , Transducers
5.
Amino acid synthesis in stimulated primitive environments.
Hulett, H R; Bar-Nun, A; Bar-Nun, N; Bauer, S H; Sagan, C.
Science ; 170(3961): 1000-2, 1970 Nov 27.
Article in English | MEDLINE | ID: mdl-5475019

Subject(s)
Amino Acids/chemical synthesis , Biological Evolution , Calorimetry , Extraterrestrial Environment , Photochemistry
6.
Tissue-typing instrumentation using the fluorochromatic cytotoxicity assay.
Hulett, H R; Coukell, A; Bodmer, W.
Transplantation ; 10(1): 135-7, 1970 Jul.
Article in English | MEDLINE | ID: mdl-5425463

Subject(s)
Fluorescence , Histocompatibility Testing , Leukocytes , Acetates , Autoanalysis , Fluoresceins , Histocompatibility Testing/instrumentation , Humans , Immune Sera , Methods , Spectrophotometry
7.
Non-enzymatic hydrolysis of adenosine phosphates.
Hulett, H R.
Nature ; 225(5239): 1248-9, 1970 Mar 28.
Article in English | MEDLINE | ID: mdl-5435354

Subject(s)
Adenine Nucleotides , Adenosine Triphosphate , Calcium , Chemical Phenomena , Chemistry , Osmolar Concentration , Seawater , Temperature , Water
8.
Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence.
Hulett, H R; Bonner, W A; Barrett, J; Herzenberg, L A.
Science ; 166(3906): 747-9, 1969 Nov 07.
Article in English | MEDLINE | ID: mdl-4898615

ABSTRACT

A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.


Subject(s)
Automation , Cell Biology , Fluorescence , Animals , Cricetinae , Culture Techniques , Female , Fluoresceins/pharmacology , Fluorescent Antibody Technique , Methods , Mice , Neoplasms, Experimental , Ovarian Neoplasms , Ovary/drug effects , Spleen/drug effects
9.
Limitations on prebiological synthesis.
Hulett, H R.
J Theor Biol ; 24(1): 56-72, 1969 Jul.
Article in English | MEDLINE | ID: mdl-5348640

Subject(s)
Biological Evolution , Chemistry , Models, Theoretical , Amino Acids/chemical synthesis , Chemical Phenomena , Cyanides/chemical synthesis , Electricity , Formaldehyde/chemical synthesis , Metabolism , Phosphates/chemical synthesis , Photosynthesis , Sunlight , Temperature , Ultraviolet Rays
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