Phytoplankton assemblage responses to nitrogen following COVID-19 stay-in-place orders in western Long Island Sound (New York/Connecticut).

This study evaluated water quality, nitrogen (N), and phytoplankton assemblage linkages along the western Long Island Sound (USA) shoreline (Nov. 2020-Dec. 2021) following COVID-19 stay-in-place (SIP) orders through monthly surveys and N-addition bioassays. Ammonia-N (AmN; NH3+NH4+) negatively correlated with total chlorophyll-a (chl-a) at all sites; this was significant at Alley Creek, adjacent to urban wastewater inputs, and at Calf Pasture, by the Norwalk River (Spearman rank correlation, p < 0.01 and 0.02). Diatoms were abundant throughout the study, though dinoflagellates (Heterocapsa, Prorocentrum), euglenoids/cryptophytes, and both nano- and picoplankton biomass increased during summer. In field and experimental assessments, high nitrite + nitrate (N + N) and low AmN increased diatom abundances while AmN was positively linked to cryptophyte concentrations. Likely N + N decreases with presumably minimal changes in AmN and organic N during COVID-19 SIP resulted in phytoplankton assemblage shifts (decreased diatoms, increased euglenoids/cryptophytes), highlighting the ecological impacts of N-form delivered by wastewater to urban estuaries.

Transitions in nitrogen and organic matter form and concentration correspond to bacterial population dynamics in a hypoxic urban estuary.

Nitrogen (N) inputs to developed coastlines are linked with multiple ecosystem and socio-economic impacts worldwide such as algal blooms, habitat/resource deterioration, and hypoxia. This study investigated the microbial and biogeochemical processes associated with recurrent, seasonal bottom-water hypoxia in an urban estuary, western Long Island Sound (WLIS), that receives high N inputs. A 2-year (2020-2021) field study spanned two hypoxia events and entailed surface and bottom depth water sampling for dissolved nutrients as inorganic N (DIN; ammonia-N and nitrite + nitrate (N + N)), organic N, orthophosphate, organic carbon (DOC), as well as chlorophyll a and bacterial abundances. Physical water quality data were obtained from concurrent conductivity, temperature, and depth casts. Results showed that dissolved organic matter was highest at the most-hypoxic locations, DOC was negatively and significantly correlated with bottom-water dissolved oxygen (Pearson's r = -0.53, p = 0.05), and ammonia-N was the dominant DIN form pre-hypoxia before declining throughout hypoxia. N + N concentrations showed the reverse, being minimal pre-hypoxia then increasing during and following hypoxia, indicating that ammonia oxidation likely contributed to the switch in dominant DIN forms and is a key pathway in WLIS water column nitrification. Similarly, at the most hypoxic sampling site, bottom depth bacteria concentrations ranged ~ 1.8 × 104-1.1 × 105 cells ml-1 pre-hypoxia, declined throughout hypoxia, and were positively and significantly correlated (Pearson's r = 0.57; p = 0.03) with ammonia-N, confirming that hypoxia influences N-cycling within LIS. These findings provide novel insight to feedbacks between major biogeochemical (N and C) cycles and hypoxia in urban estuaries. Supplementary Information: The online version contains supplementary material available at 10.1007/s10533-023-01021-2.