ABSTRACT
The sex pheromone of the mealybug, Planococcus minor was isolated by fractionation of crude pheromone extract obtained by aeration of virgin females. The pheromone was identified as the irregular terpenoid, 2-isopropyl-5-methyl-2,4-hexadienyl acetate, by mass spectrometry, microchemical tests, and (1)H NMR spectroscopy. The stereochemistry of the pheromone was assigned as (E) by comparison with synthetic standards of known geometry. The compound was highly attractive to males in laboratory bioassays, whereas the (Z)-isomer appeared to antagonize attraction.
Subject(s)
Hemiptera/metabolism , Sex Attractants/chemical synthesis , Animals , Female , Male , Molecular Structure , Sex Attractants/pharmacology , Sexual Behavior, Animal/drug effectsABSTRACT
A simple and efficient technique that does not require solvent and uses less operating time for the investigation of sex pheromones of the carambola fruit borer (Eucosma notanthes Meyrick) by utilizing headspace solid-phase microextraction (SPME) followed by GC-MS analysis has been developed. Variables such as types of SPME fiber, number of pests, temperature and extraction time have been studied. Whole sex glands of Eucosma notanthes Meyrick were dissected from 5 virgin insects, placed in a 2mL vial, equilibrated at 170 degrees C for 10min, and then extracted by headspace SPME at room temperature for 5min. The results of the GC-MS analyses of headspace SPME of these sex glandular solid samples were much better than those obtained with hexane extraction of sex glandular from 117 insects followed by either headspace SPME or direct injection due to higher absorption efficiency. The simplicity of this technique renders it a very suitable method for research on the biological control of pests.