ABSTRACT
An analysis of the assay of 28 preparations of anti-D immunoglobulin using a radioisotope method carried out at 6-montly intervals for 2--4.5 years showed an average fall in anti-D concentration of 10.6% each year, with 99% confidence limits of 6.8--14.7%. The fall in anti-D concentration after storage at 37 degrees C for 1 month was less than 8%, the minimum change that could be detected. No significant change in physical characteristics of the immunoglobulin were detected. The error of a single estimate of anti-D by the radioisotope method (125I-labelled anti-IgG) used here was calculated to be such that the true value probably (p = 0.95) lay between 66 and 150% of the estimated value.
Subject(s)
Antibodies, Anti-Idiotypic , Rh-Hr Blood-Group System , Cold Temperature , Drug Stability , Hot Temperature , Humans , Immunoglobulin G , Preservation, Biological , Time FactorsABSTRACT
The degradation of red cell stroma phospholipids by phospholipase A2 is accompanied by a concomitant fall in the activity of the Rh antigens, c, D and e. The action of phospholipase C on stroma also brings about a fall in D antigen activity. Anti-D bound to the red cells protects the D antigen from inactivation by phospholipase A2.