ABSTRACT
This study was aimed to screen human papillomavirus (HPV) types associated with esophageal squamous cell carcinoma of Kazakh in Xinjiang using the gene chip technique and study the clinical significance of this application. The DNAs were collected from esophageal squamous cell carcinoma tissues and healthy esophageal mucosa of Kazakh adults in Xinjiang, and amplified firstly using HPV MY09/11 and then using HPV G5+/6+ to screen positive HPV specimens. These positive specimens were further detected by the gene chip technique to screen highly pathogenic HPV types. After determination with nested PCR amplification with HPV MY09/11 and G5+/6+, the infection rate of HPV was 66.67% in the esophageal squamous cell carcinoma group and 12.12% in the healthy control group. By testing the positive HPV specimens from the esophageal squamous cell carcinoma group, the infection rate of HPV16 was 97.72% and the co-infection rate of HPV16 and HPV18 was 2.27%. HPV16 infection may be involved in the development of esophageal squamous cell carcinoma in Xinjiang Hazakh adults.
Subject(s)
Carcinoma, Squamous Cell/genetics , Esophageal Neoplasms/genetics , Oligonucleotide Array Sequence Analysis/methods , Papillomaviridae/genetics , Papillomavirus Infections/genetics , Aged , Asian People , Carcinoma, Squamous Cell/ethnology , Carcinoma, Squamous Cell/virology , Case-Control Studies , China , DNA, Neoplasm/analysis , DNA, Neoplasm/genetics , DNA, Viral/analysis , DNA, Viral/genetics , Esophageal Neoplasms/ethnology , Esophageal Neoplasms/virology , Female , Host-Pathogen Interactions/genetics , Human papillomavirus 16/genetics , Human papillomavirus 18/genetics , Humans , Male , Middle Aged , Molecular Typing/methods , Molecular Typing/statistics & numerical data , Papillomaviridae/classification , Papillomaviridae/physiology , Papillomavirus Infections/ethnology , Papillomavirus Infections/virology , Polymerase Chain ReactionABSTRACT
The methanol extract from roots of Salacia hainanensis Chun et How afforded three new compounds, 24,26-dihydroxy-25-methoxy-tirucall-9-en-3-one (1), 2ß,3ß,22α-trihydroxy-lup-20(29)-ene (2) and 3ß-hydroxy-2-carbonyl-lupan-29-oic acid (3), along with six known triterpenoids (4-9). Their structures were established on the basis of spectral analysis, in particular according to the data obtained by two-dimensional-NMR and high-resolution mass spectra experiments. Some of them showed much stronger inhibitory activity towards α-glucosidase than the positive control (acarbose, IC50 10.2 µM).
