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1.
Nat Methods ; 20(5): 761-769, 2023 05.
Article in English | MEDLINE | ID: mdl-37024651

ABSTRACT

Neuronal computation is achieved through connections of individual neurons into a larger network. To expand the repertoire of endogenous cellular communication, we developed a synthetic, photon-assisted synaptic transmission (PhAST) system. PhAST is based on luciferases and channelrhodopsins that enable the transmission of a neuronal state across space, using photons as neurotransmitters. PhAST overcomes synaptic barriers and rescues the behavioral deficit of a glutamate mutant with conditional, calcium-triggered photon emission between two neurons of the Caenorhabditis elegans nociceptive avoidance circuit. To demonstrate versatility and flexibility, we generated de novo synaptic transmission between two unconnected cells in a sexually dimorphic neuronal circuit, suppressed endogenous nocifensive response through activation of an anion channelrhodopsin and switched attractive to aversive behavior in an olfactory circuit. Finally, we applied PhAST to dissect the calcium dynamics of the temporal pattern generator in a motor circuit for ovipositioning. In summary, we established photon-based synaptic transmission that facilitates the modification of animal behavior.


Subject(s)
Calcium , Photons , Animals , Neurons/physiology , Synaptic Transmission , Caenorhabditis elegans/physiology
2.
Infect Dis Rep ; 13(4): 1061-1077, 2021 Dec 14.
Article in English | MEDLINE | ID: mdl-34940407

ABSTRACT

Since the outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in December 2019, the spread of SARS-CoV2 infection has been escalating rapidly around the world. In order to provide more timely access to medical intervention, including diagnostic tests and medical treatment, the FDA authorized multiple test protocols for diagnostic tests from nasopharyngeal swab, saliva, urine, bronchoalveolar lavage and fecal samples. The traditional diagnostic tests for this novel coronavirus 2019 require standard processes of viral RNA isolation, reverse transcription of RNA to cDNA, then real-time quantitative PCR with the RNA templates extracted from the patient samples. Recently, many reports have demonstrated a direct detection of SARS-Co-V2 genomic material from saliva samples without any RNA isolation step. To make the rapid detection of SARS-Co-V2 infection more accessible, a point-of-care type device was developed for SARS-CoV-2 detection. Herein, we report a portable microfluidic-based integrated detection-analysis system for SARS-CoV-2 nucleic acids detection directly from saliva samples. The saliva cartridge is self-contained and capable of microfluidic evaluation of saliva, from heating, mixing with the primers to multiplex real-time quantitative polymerase chain reaction, detecting SARS-CoV-2 with different primer sets and internal control. The approach has a detection sensitivity of 1000 copies/mL of SARS-CoV-2 RNA or virus, with consistency and automation, from saliva sample-in to result-out.

3.
Biomed Opt Express ; 10(7): 3698, 2019 Jul 01.
Article in English | MEDLINE | ID: mdl-31467799

ABSTRACT

[This corrects the article on p. 2101 in vol. 10, PMID: 31061773.].

4.
Biomed Opt Express ; 10(4): 2101-2116, 2019 Apr 01.
Article in English | MEDLINE | ID: mdl-31061773

ABSTRACT

Monitoring the early onset of bacterial film formation is critical in many clinical, environmental, and food quality control applications. We built a small inexpensive optical surface cytometer, in contrast with bulk spectroscopic methods, around a light-emitting diode (LED) and a complementary metal-oxide-semiconductor (CMOS) image sensor. It is designed to offer a large field-of-view of 200 mm2 and a large depth-of-field of 2-3 mm to overcome the limitations of routine methods like spectrophotometry and fluorescence microscopy. It provides a direct measurement without the need for complex image post-processing with a limit-of-detection around 104 cells/mm2, which is competitive with other similar yet more complex devices already available.

5.
Methods Mol Biol ; 1906: 207-224, 2019.
Article in English | MEDLINE | ID: mdl-30488395

ABSTRACT

A fully automated rapid DNA analysis system requires integrating several operational elements performing multiple steps onto one single microfluidic platform. The functions to include on the microfluidic platform consist of substrate lysis, lysate DNA extraction, single or multiplexed PCR amplification, amplicon separation, and product readout. Here we describe a fully automated integrated system for forensic short tandem repeat (STR) analysis of reference samples, achieving buccal swab-in and DNA profile-out.


Subject(s)
DNA Fingerprinting/instrumentation , Microfluidic Analytical Techniques/instrumentation , Microsatellite Repeats , DNA Fingerprinting/methods , Electrophoresis, Capillary , Equipment Design , Forensic Genetics , Humans , Microfluidic Analytical Techniques/methods , Mouth/chemistry , Multiplex Polymerase Chain Reaction , Specimen Handling
6.
Biomed Microdevices ; 16(6): 905-14, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25106501

ABSTRACT

We report a microfluidic device and measurement method to perform real-time PCR (or qPCR) in a miniaturized configuration for on-chip implementation using reaction volumes of less than 20 µL. The qPCR bioreactor is designed as a module to be embedded in an automated sample-in/profile-out system for rapid DNA biometrics or human identification. The PCR mixture is excited with a 505 nm diode-pumped solid-state laser (DPSSL) and the fluorescence build-up is measured using optical fibers directly embedded to the sidewalls of the microfluidic qPCR bioreactor. We discuss manufacturing and operating parameters necessary to adjust the internal surface conditions and temperature profiles of the bioreactor and to optimize the yield and quality of the PCR reaction for the amplification of 62 bp hTERT intron fragments using the commercial Quantifiler® kit (Life Technologies, Carlsbad, CA) commonly accepted for genotyping analysis. We designed a microfluidic device suitable for continuously processing a specimen by efficiently mixing the reagents from the kit to a set volume of DNA template on chip. Our approach relies on a calibration curve for the specific device using control DNA. We successfully applied this method to determine the concentration of genomic DNA extracted from a buccal swab on separate microfluidic devices which are operated upstream the qPCR device and perform buccal swab lysis and buccal DNA extraction. A precise correlation between the amount determined on chip and that obtained using a commercial cycler is demonstrated.


Subject(s)
Genotyping Techniques , Lab-On-A-Chip Devices , Microfluidic Analytical Techniques , Real-Time Polymerase Chain Reaction , DNA/chemistry , DNA/genetics , DNA/isolation & purification , Female , Genotyping Techniques/instrumentation , Genotyping Techniques/methods , Humans , Lasers, Semiconductor , Male , Microfluidic Analytical Techniques/instrumentation , Microfluidic Analytical Techniques/methods , Mouth Mucosa , Real-Time Polymerase Chain Reaction/instrumentation , Real-Time Polymerase Chain Reaction/methods , Telomerase/genetics
7.
Forensic Sci Int Genet ; 8(1): 147-58, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24315603

ABSTRACT

Fully automated rapid forensic DNA analysis requires integrating several multistep processes onto a single microfluidic platform, including substrate lysis, extraction of DNA from the released lysate solution, multiplexed PCR amplification of STR loci, separation of PCR products by capillary electrophoresis, and analysis for allelic peak calling. Over the past several years, most of the rapid DNA analysis systems developed started with the reference swab sample lysate and involved an off-chip lysis of collected substrates. As a result of advancement in technology and chemistry, addition of a microfluidic module for swab sample lysis has been achieved in a few of the rapid DNA analysis systems. However, recent reports on integrated rapid DNA analysis systems with swab-in and answer-out capability lack any quantitative and qualitative characterization of the swab-in sample lysis module, which is important for downstream forensic sample processing. Maximal collection and subsequent recovery of the biological material from the crime scene is one of the first and critical steps in forensic DNA technology. Herein we present the design, fabrication and characterization of an integratable swab lysis cartridge module and the test results obtained from different types of commonly used forensic swab samples, including buccal, saliva, and blood swab samples, demonstrating the compatibility with different downstream DNA extraction chemistries. This swab lysis cartridge module is easy to operate, compatible with both forensic and microfluidic requirements, and ready to be integrated with our existing automated rapid forensic DNA analysis system. Following the characterization of the swab lysis module, an integrated run from buccal swab sample-in to the microchip CE electropherogram-out was demonstrated on the integrated prototype instrument. Therefore, in this study, we demonstrate that this swab lysis cartridge module is: (1) functionally, comparable with routine benchtop lysis, (2) compatible with various types of swab samples and chemistries, and (3) integratable to achieve a micro total analysis system (µTAS) for rapid DNA analysis.


Subject(s)
Forensic Genetics , Microfluidics/instrumentation , Feasibility Studies , Microsatellite Repeats/genetics , Polymerase Chain Reaction
8.
Phys Chem Chem Phys ; 15(31): 12805-14, 2013 Aug 21.
Article in English | MEDLINE | ID: mdl-23636584

ABSTRACT

We report an on-chip gradient generator that has been designed, modelled, fabricated, and characterized to facilitate temporal tuning of several unique gradients in parallel for multiple applications. This design allows for steady state programming of the intensities across multiple orders of magnitude while producing exponential, linear, and logarithmic gradient profiles. The magnitude of the gradients is controlled through regulating the ratio of the two on-chip flow inlets without the need for valves or other active mixers. On-chip binding of biotin by a fluorescent streptavidin complex creates a diffusive barrier that regulates access to the gradient inlets, providing a second orthogonal mechanism for regulating the microgradient intensities. The device is also characterized using an on-chip enzymatic reaction to produce an array of tuneable product concentrations within the various microchannels.


Subject(s)
Microfluidic Analytical Techniques/instrumentation , Equipment Design
9.
Electrophoresis ; 33(16): 2604-11, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22899270

ABSTRACT

We report the design and performance validation of microfluidic separation technologies for human identification using a disposable plastic device suitable for integration into an automated rapid DNA analysis system. A fabrication process for a 15-cm long hot-embossed plastic microfluidic devices with a smooth semielliptical cross section out of cyclic olefin copolymer is presented. We propose a mixed polymer solution of 95% w/v hydroxyethylcellulose and 5% w/v polyvinylpyrrolidone for a final polymer concentration of 2.5 or 3.0% to be used as coating and sieving matrix for DNA separation. This formulation allows preparing the microchip without pretreatment in a single-loading step and provides high-resolution separation (≈1.2 bp for fragments <200 bp), which is superior to existing commercial matrices under the same conditions. The hot-embossed device performance is characterized and compared to injection-molded devices made out of cyclic olefin copolymer based on their respective injector geometry, channel shape, and surface charges. Each device design is assessed by fluorescence videomicroscopy to evaluate the formation of injection plugs, then by comparing electropherograms for the separation of a DNA size standard relevant to human identification.


Subject(s)
DNA/analysis , Electrophoresis, Capillary/instrumentation , Microfluidic Analytical Techniques/instrumentation , Microfluidic Analytical Techniques/methods , Povidone/chemistry , DNA/isolation & purification , Electrophoresis, Capillary/methods , Equipment Design , Humans , Kinetics , Spectrometry, Fluorescence
10.
Electrophoresis ; 31(21): 3510-7, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20931618

ABSTRACT

The microfluidic integration of an entire DNA analysis workflow on a fully integrated miniaturized instrument is reported using lab-on-a-chip automation to perform DNA fingerprinting compatible with CODIS standard relevant to the forensic community. The instrument aims to improve the cost, duration, and ease of use to perform a "sample-to-profile" analysis with no need for human intervention. The present publication describes the operation of the three major components of the system: the electronic control components, the microfluidic cartridge and CE microchip, and the optical excitation/detection module. Experimental details are given to characterize the level of performance, stability, reliability, accuracy, and sensitivity of the prototype system. A typical temperature profile from a PCR amplification process and an electropherogram of a commercial size standard (GeneScan 500™, Applied Biosystems) separation are shown to assess the relevance of the instrument to forensic applications. Finally, we present a profile from an automated integrated run where lysed cells from a buccal swab were introduced in the system and no further human intervention was required to complete the analysis.


Subject(s)
Electrophoresis, Capillary/methods , Microfluidic Analytical Techniques/instrumentation , Microsatellite Repeats , Polymerase Chain Reaction/methods , Sequence Analysis, DNA/instrumentation , Cheek , DNA/chemistry , DNA/isolation & purification , Forensic Genetics/methods , Humans , Microfluidic Analytical Techniques/methods , Mouth Mucosa/cytology , Reproducibility of Results , Sequence Analysis, DNA/methods , Temperature
11.
Anal Chem ; 82(16): 6991-9, 2010 Aug 15.
Article in English | MEDLINE | ID: mdl-20704389

ABSTRACT

We demonstrate a conduit for the delivery of a step change in the DNA analysis process: A fully integrated instrument for the analysis of multiplex short tandem repeat DNA profiles from reference buccal samples is described and is suitable for the processing of such samples within a forensic environment such as a police custody suite or booking office. The instrument is loaded with a DNA processing cartridge which incorporates on-board pumps and valves which direct the delivery of sample and reagents to the various reaction chambers to allow DNA purification, amplification of the DNA by PCR, and collection of the amplified product for delivery to an integral CE chip. The fluorescently labeled product is separated using micro capillary electrophoresis with a resolution of 1.2 base pairs (bp) allowing laser induced fluorescence-based detection of the amplified short tandem repeat fragments and subsequent analysis of data to produce a DNA profile which is compatible with the data format of the UK DNA database. The entire process from taking the sample from a suspect, to database compatible DNA profile production can currently be achieved in less than 4 h. By integrating such an instrument and microfluidic cartridge with the forensic process, we believe it will be possible in the near future to process a DNA sample taken from an individual in police custody and compare the profile with the DNA profiles held on a DNA Database in as little as 3 h.


Subject(s)
DNA/analysis , Forensic Genetics/methods , Microfluidic Analytical Techniques/methods , Databases, Nucleic Acid , Polymerase Chain Reaction , Specimen Handling , Time Factors
12.
Biosens Bioelectron ; 22(11): 2449-55, 2007 May 15.
Article in English | MEDLINE | ID: mdl-17045469

ABSTRACT

This work reports the first evidence that recombinant yeast phosphoglycerate kinase (PGK) is still significantly active when immobilized on glass and muscovite mica. Using previous work to improve the sensitivity of the existing setup, Tapping Mode atomic force microscopy (AFM) was used in a liquid environment to determine the surface enzyme coverage of derivatized mica and glass slides. When associated to spectrophotometric measurements, the AFM data allows assessing the catalytic constant of surface enzymes and comparing it to bulk values. The validity of the Michaelis-Menten model for surface reactions is discussed, supported by spectroscopic measurements of the surface consumption of 1,3-bis-phosphoglycerate (1,3-BPG). Only a few percent of the enzyme material maintains its initial bulk activity. This value could constitute a guideline for biosensors made with the method used here whenever a rapid assessment of the remaining surface activity is needed.


Subject(s)
Biocompatible Materials/chemistry , Biosensing Techniques/methods , Phosphoglycerate Kinase/chemistry , Yeasts/enzymology , Aluminum Silicates/chemistry , Enzyme Activation , Enzymes, Immobilized/chemistry , Glass/chemistry , Phosphoglycerate Kinase/ultrastructure , Surface Properties
13.
J Phys Chem B ; 109(19): 9532-9, 2005 May 19.
Article in English | MEDLINE | ID: mdl-16852147

ABSTRACT

A scanning electrochemical microscopy (SECM) approach for the analysis of heterogeneous catalytic reactions at solid-liquid interfaces is described and applied. In this scheme, reactant, generated at a tip, undergoes a reaction (e.g., disproportionation) at the substrate. The theoretical background for this study, performed by digital simulations using a finite difference method, considers a chemical reaction at the substrate with general stoichiometry. In this case, the fraction of regenerated mediator (nu(S)) may differ with respect to a substrate reaction that is the reverse of the tip reaction, resulting in an asymmetric mediator loop. Simulated tip current transients and approach curves at different values of the kinetic rate constant for reactions where nu(S) < 1 were used to analyze this new SECM situation. This approach was used to study the catalytic decomposition of hydrogen peroxide (HO2- --> 1/2O2 + OH-), where nu(S) = 0.5, on supported catalysts. A gold-mercury amalgam tip was used to quantitatively reduce dissolved O2 (mediator) to HO2-, which was decomposed back to oxygen at the catalyst substrate. Rate constants for the decomposition reaction on immobilized catalase and Pt particles were measured at different pH values by the correlation of experimental approach curves with the theoretical dependencies.

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