ABSTRACT
In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.
Subject(s)
Antimycin A/metabolism , Isoflavones/biosynthesis , Streptomyces/metabolism , Antimycin A/analogs & derivatives , Genistein/metabolism , Streptomyces/chemistry , Streptomyces/genetics , Streptomyces/ultrastructureABSTRACT
An impedimetric glycan biosensor with optimised glycan density was applied for the detection of lectins and influenza hemagglutinins down to attomolar concentrations (aM).
Subject(s)
Biosensing Techniques , Hemagglutinin Glycoproteins, Influenza Virus/analysis , Lectins/analysis , Polysaccharides/analysis , Humans , Influenza A Virus, H1N1 Subtype/metabolism , Influenza A Virus, H5N1 Subtype/metabolism , Maackia/metabolism , Microscopy, Atomic Force , Phytohemagglutinins/analysisABSTRACT
Complex carbohydrates (glycans) play an important role in nature and study of their interaction with proteins or intact cells can be useful for understanding many physiological and pathological processes. Such interactions have been successfully interrogated in a highly parallel way using glycan microarrays, but this technique has some limitations. Thus, in recent years glycan biosensors in numerous progressive configurations have been developed offering distinct advantages compared to glycan microarrays. Thus, in this review advances achieved in the field of label-free glycan biosensors are discussed.
