Nested capillary anti-resonant silica fiber with mid-infrared transmission and low bending sensitivity at 4000 nm.

We report a silica glass nested capillary anti-resonant nodeless fiber with transmission and low bending sensitivity in the mid-infrared around 4000 nm. The fiber is characterized in terms of transmission over 1700-4200 nm wavelengths, revealing a mid-infrared 3500-4200 nm transmission window, clearly observable for a 12 m long fiber. Bending loss around 4000 nm is 0.5 dB/m measured over three full turns with 40 mm radius, going up to 5 dB/m for full turns with 15 mm radius. Our results provide experimental evidence of hollow-core silica fibers in which nested, anti-resonant capillaries provide high bend resistance in the mid-infrared. This is obtained for a fiber with a large core diameter of over 60 µm relative to around 30 µm capillaries in the cladding, which motivates its application in gas fiber lasers or fiber-based mid-infrared spectroscopy of COx or NxO analytes.

Chemical imaging of lipid droplets in muscle tissues using hyperspectral coherent Raman microscopy.

The accumulation of lipids in non-adipose tissues is attracting increasing attention due to its correlation with obesity. In muscle tissue, ectopic deposition of specific lipids is further correlated with pathogenic development of insulin resistance and type 2 diabetes. Most intramyocellular lipids are organized into lipid droplets (LDs), which are metabolically active organelles. In order to better understand the putative role of LDs in pathogenesis, insight into both the location of LDs and nearby chemistry of muscle tissue is very useful. Here, we demonstrate the use of label-free coherent anti-Stokes Raman scattering (CARS) microscopy in combination with multivariate, chemometric analysis to visualize intracellular lipid accumulations in ex vivo muscle tissue. Consistent with our previous results, hyperspectral CARS microscopy showed an increase in LDs in tissues where LD proteins were overexpressed, and further chemometric analysis showed additional features morphologically (and chemically) similar to mitochondria that colocalized with LDs. CARS imaging is shown to be a very useful method for label-free stratification of ectopic fat deposition and cellular organelles in fresh tissue sections with virtually no sample preparation.

Flow cytometer based on triggered supercontinuum laser illumination.

Multiple wavelength operation in a flow cytometer is an exciting way for cell analysis based on both fluorescence and optical scattering processing. For example, this multiparametric technique is currently used to differentiate blood cells subpopulations. The choice of excitation wavelengths matching fluorochrome spectra (it is currently the opposite) and the use of a broader range of fluorochromes can be made by taking advantage of a filtered supercontinuum white light source. In this study, we first wished to validate the use of a specific triggered supercontinuum laser in a flow cytometer based on white light scattering and electric sizing on human blood cells. Subsequently, to show the various advantages of this attractive system, using scattering effect, electrical detections, and fluorescence analysis, we realized cells sorting based on DNA/RNA stained by thiazole orange. Discrimination of white blood cells is efficiently demonstrated by using a triggered supercontinuum-based flow cytometer operating in a "one cell-one shot" configuration. The discriminated leukocyte populations are monocytes, lymphocytes, granulocytes, immature granulocytes, and cells having a high RNA content (monoblasts, lymphoblasts, and plasma cells). To the best of our knowledge, these results constitute the first practical demonstration of flow cytometry based on triggered supercontinuum illumination. This study is the starting point of a series of new experiments fully exploiting the spectral features of such a laser source. For example, the large flexibility in the choice of the excitation wavelength allows to use a larger number of fluorochromes and to excite them more efficiently. Moreover, this work opens up new research directions in the biophotonics field, such as the combination of coherent Raman spectroscopy and flow cytometry techniques.

Ultra wide band supercontinuum generation in air-silica holey fibers by SHG-induced modulation instabilities.

Second harmonic generation in an air-silica microstructured optical fiber pumped by subnanosecond pulses is used in order to initiate modulation instability processes in normal and anomalous dispersion regimes. This allows us to generate an ultra wide and flat supercontinuum (350-1750 nm), covering the entire transparency window of silica and exhibiting a singlemode transverse profile in visible range.