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1.
J Genet Eng Biotechnol ; 13(2): 145-155, 2015 Dec.
Article in English | MEDLINE | ID: mdl-30647578

ABSTRACT

The microalga biomass production from confectionary effluent is a possible solution for the urgent need for a live food in aquaculture. Arthrospira (Spirulina) platensis was the dominant alga in effluent of "Biscomisr a confectionary factory", in Alexandria-Egypt. Therefore, it was isolated from the effluent samples and used throughout the study. The cyanobacterium, A. platensis was grown on the effluent using 22 Central Composite Design (22 CCD). This work addresses the best effluent dilution (WC, %) as well as sodium bicarbonate concentration (SBC) on the alga growth and biochemical composition. Total protein, carbohydrate, lipid contents and fatty acid profiles of the produced algal biomass were highly improved. The statistical analyses suggested that the main effect of (WC, %) is significant negative influences on the algal contents of proteins, lipids and carbohydrates (p > 0.01). Although it had a significant positive influence on chlorophyll (p > 0.01), no significant effect on algal ß carotenes (p > 0.05) had been reported. The inter action effect of SBC together with WC, % exerted a significant negative influence on the algal proteins (p > 0.01) and no significant effect on the other responses (p > 0.05). The produced alga biomass was used for feeding the rotifer, Brachionus plicatilis for further application in aquaculture. Growth rate, reproductive rate and fecundity attributes, fatty acid content of B. plicatilis were amended. The Pearson correlation test indicated that ß carotenes displayed a highly positive significant correlation with the growth rate of B. plicatilis (r = 0.733, p < 0.01) and the carbohydrates showed significant positive correlations with Egg % (r = 0.657, p < 0.05).

2.
J Environ Biol ; 33(1): 101-5, 2012 Jan.
Article in English | MEDLINE | ID: mdl-23033651

ABSTRACT

Phytoplankton was assessed quantitatively and qualitatively in regard to their abundance in the northern part of El-Umum drain, west of Alexandria at four stations to study the spatial, temporal, dominance and abundance of the phytoplankton community. The samples were collected monthly from April 2003 to April 2004. Atotal of 152 phytoplankton taxa were identified belonging to Bacillariophyceae (60), Chlorophyceae (46), Cyanophyceae (20), Euglenophyceae (17) and Dinophyceae (9). A limited number of these were recorded throughout the year, and showed an indication of organic pollution. The total densities (333.8x10(3) units l(-1)) were mainly a reflection of the trends in counts of Bacillariophyceae and Chlorophyceae (138.1 x 10(3) and 131.6 x 10(3) units l(-1), respectively). Monthly differences in the quantitative (18 x 10(3) to 1645 x 10(3) units l(-1)) and qualitative composition (41 to 113 taxa) of the phytoplankton communities in the different stations were marked, with recurrent high abundance in spring (910.4 x 10(3) units l(-1)), whereas lowest densities occurred in autumn (99.7x10(3) units l(-1)). Cyanophyceae was most abundant in July (67.5 x 10(3) units l(-1)), whereas Euglenophyceae in December (32.0x10(3) units l(-1)). Species diversity (H') gave a signal of the area is light to moderate polluted. The present study emphasized the need to use phytoplankton community as index of water quality.


Subject(s)
Drainage, Sanitary , Phytoplankton/physiology , Biodiversity , Demography , Egypt , Phytoplankton/classification , Population Density , Seasons , Time Factors , Water Microbiology
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