Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
Mol Oral Microbiol ; 31(6): 472-485, 2016 12.
Article in English | MEDLINE | ID: mdl-26441291

ABSTRACT

Porphyromonas gingivalis is the main causative agent of periodontitis. It deregulates the inflammatory and innate host immune responses through virulence factors, which include the immunodominant outer-membrane surface receptor antigens A (PgRagA) and B (PgRagB), co-transcribed from the rag pathogenicity island. The former is predicted to be a Ton-dependent porin-type translocator but the targets of this translocation and the molecular function of PgRagB are unknown. Phenomenologically, PgRagB has been linked with epithelial cell invasion and virulence according to murine models. It also acts as a Toll-like receptor agonist and promotes multiple mediators of inflammation. Hence, PgRagB is a candidate for the development of a periodontitis vaccine, which would be facilitated by the knowledge of its atomic structure. Here, we crystallized and solved the structure of 54-kDa PgRagB, which revealed a single domain centered on a curved helical scaffold. It consists of four tetratrico peptide repeats (TPR1-4), each arranged as two helices connected by a linker, plus two extra downstream capping helices. The concave surface bears four large intertwined irregular inserts (A-D), which contribute to an overall compact moiety. Overall, PgRagB shows substantial structural similarity with Bacteroides thetaiotaomicron SusD and Tannerella forsythia NanU, which are, respectively, engaged in binding and uptake of malto-oligosaccharide/starch and sialic acid. This suggests a similar sugar-binding function for PgRagB for uptake by the cognate PgRagA translocator, and, consistently, three potential monosaccharide-binding sites were tentatively assigned on the molecular surface.


Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Models, Molecular , Porphyromonas gingivalis/chemistry , Bacterial Proteins/immunology , Bacterial Proteins/isolation & purification , Bacteroides thetaiotaomicron/chemistry , Crystallization , Humans , N-Acetylneuraminic Acid/metabolism , Periodontitis/immunology , Periodontitis/microbiology , Porphyromonas gingivalis/immunology , Porphyromonas gingivalis/pathogenicity , Starch/metabolism , Tannerella forsythia/chemistry , Virulence Factors
2.
Mol Oral Microbiol ; 31(4): 354-64, 2016 Aug.
Article in English | MEDLINE | ID: mdl-26358096

ABSTRACT

Porphyromonas gingivalis is a Gram-negative anaerobe and keystone periodontal pathogen. A mariner transposon insertion mutant library has recently been used to define 463 genes as putatively essential for the in vitro growth of P. gingivalis ATCC 33277 in planktonic culture (Library 1). We have independently generated a transposon insertion mutant library (Library 2) for the same P. gingivalis strain and herein compare genes that are putatively essential for in vitro growth in complex media, as defined by both libraries. In all, 281 genes (61%) identified by Library 1 were common to Library 2. Many of these common genes are involved in fundamentally important metabolic pathways, notably pyrimidine cycling as well as lipopolysaccharide, peptidoglycan, pantothenate and coenzyme A biosynthesis, and nicotinate and nicotinamide metabolism. Also in common are genes encoding heat-shock protein homologues, sigma factors, enzymes with proteolytic activity, and the majority of sec-related protein export genes. In addition to facilitating a better understanding of critical physiological processes, transposon-sequencing technology has the potential to identify novel strategies for the control of P. gingivalis infections. Those genes defined as essential by two independently generated TnSeq mutant libraries are likely to represent particularly attractive therapeutic targets.


Subject(s)
Bacterial Proteins/genetics , DNA Transposable Elements , Gene Library , Genes, Bacterial , Heat-Shock Proteins/genetics , Porphyromonas gingivalis/genetics , Sigma Factor/genetics , Chromosome Mapping/methods , Genes, Essential , High-Throughput Nucleotide Sequencing/methods , Lipopolysaccharides/biosynthesis , Mutagenesis, Insertional , Mutation , Periodontal Diseases/microbiology , Porphyromonas gingivalis/growth & development , Pyrimidines/metabolism
3.
Mol Oral Microbiol ; 30(3): 242-52, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25418117

ABSTRACT

Periodontal diseases are semi-ubiquitous and caused by chronic, plaque-induced inflammation. The 55-kDa immunodominant RagB outer membrane protein of Porphyromonas gingivalis, a keystone periodontal pathogen, has been proposed to facilitate nutrient transport. However, potential interactions between RagB and the innate response have not been examined. We determined that RagB exposure led to the differential and dose-related expression of multiple genes encoding proinflammatory mediators [interleukin-1α (IL-1α), IL-1ß, IL-6, IL-8 and CCL2; all P < 0.05] in primary human monocytes and to the secretion of tumor necrosis factor and IL-8, but not interferon-γ or IL-12. RagB was shown to be a Toll-like receptor 2 (TLR2) and TLR4 agonist that activated signal transducer and activator of transcription 4 and nuclear factor-κB signaling, as determined by a combination of blocking antibodies, pharmaceutical inhibitors and gene silencing. In keeping, a ΔragB mutant similarly exhibited reduced inflammatory capacity, which was rescued by ragB complementation. These results suggest that RagB elicits a major pro-inflammatory response in primary human monocytes and, therefore, could play an important role in the etiology of periodontitis and systemic sequelae.


Subject(s)
Bacterial Proteins/immunology , Porphyromonas gingivalis/genetics , STAT4 Transcription Factor/agonists , Toll-Like Receptor 2/agonists , Toll-Like Receptor 4/agonists , Bacterial Proteins/genetics , Cytokines/immunology , Gingiva/immunology , Humans , Lipopolysaccharides/immunology , Monocytes/immunology , NF-kappa B/immunology , Periodontitis/immunology , Phosphorylation , Signal Transduction/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...