A serial study of heart function during pregnancy, lactation and the dry period in dairy goats using echocardiography.

Echocardiography and Doppler measurements were performed to investigate changes in heart dimensions and function during pregnancy, lactation and the non-pregnant, non-lactating (dry) period in dairy goats. Eight Swedish domestic goats (Capra hircus) were studied with two-dimensional (2DE), M-mode and continuous wave Doppler measurements. Cardiac dimensions did not differ between periods. The mean cardiac outputs were 35 and 28% greater during pregnancy and lactation, respectively, vs. the dry period. Arterial blood pressure (measured by telemetry) did not differ between reproductive periods before echocardiographic measurements, but became elevated during the measurements, with the smallest rise during lactation. Heart rate was elevated during pregnancy and increased further during echocardiographic measurements, but was not elevated during the other periods. The cranial location of the heart influenced the selection of examination window and caused some difficulties in positioning the ultrasound transducer. This may have led to underestimation of the velocity trace integral and therefore to underestimation of cardiac output. However, our values were within the range of these obtained with dye- and thermodilution methods. The goats tolerated the investigations well during lactation and the dry period, but showed increased blood pressure and heart rate during pregnancy. Therefore, the non-invasive and safe echocardiographic-Doppler method appears to be a good alternative for studies of heart dimensions and function during lactation and the dry period in the dairy goat.

Telemetric registration of heart rate and blood pressure in the same unrestrained goats during pregnancy, lactation and the non-pregnant, non-lactating period.

The purpose of this study was to investigate how changes in heart rate and arterial blood pressure relate to the time of day, reproductive period and feeding routines in dairy goats (Capra hircus). Registrations were made by radiotelemetry in the same four goats during pregnancy, lactation and the non-pregnant, non-lactating (dry) period. Heart rate rose around the morning and afternoon feedings, whereas blood pressure did not show any diurnal rhythm. Comparison between reproductive periods revealed that heart rate was higher during the fifth month of pregnancy than during lactation and the dry period, whereas for blood pressure no such differences between periods were found. Withholding three meals from lactating goats resulted in a continuous slowdown of the heart rate, whereas blood pressure fluctuated. Re-feeding temporarily increased the heart rate but had no effect on blood pressure which continued to fluctuate. After another 2 days, blood pressure (but not heart rate) had stabilized. Food-restriction, aimed at terminating milk production resulted in a consistently depressed heart rate and reduced the mean and systolic blood pressures at night. The results show that with this implantable telemetry device it is possible to measure both heart rate and blood pressure day and night in the same unrestrained animals over a length of time long enough to include all reproductive periods. Our results emphasize that when planning experiments it is important that the exact stage in each reproductive period, the act of feeding and the amount of food given be taken into account.

Hormonal changes during parturition in heifers and goats are related to the phases and severity of labour.

Parturition is a natural event that involves stress and pain for the mother. We thus hypothesized that levels of stress hormones measured during parturition could reflect levels reached in response to severe discomfort and pain of other kinds as well. The aim of this study was therefore to determine whether plasma concentrations of cortisol, adrenaline, noradrenaline, beta-endorphin, met-enkephalin, vasopressin and oxytocin vary depending on the phase and severity of labour in dairy heifers (ten) and dairy goats (six), and how these hormones interact with each other. Blood samples were taken once a day for 3 days before labour and for 3 days afterwards and at predetermined phases during labour. All heifers delivered one calf and five of them needed obstetrical assistance. Two of the goats delivered one kid, and four had twins; all kidded without help. The cortisol concentration peaked when the calf and the first kid were born. In the heifers, plasma adrenaline increased after delivery, while the noradrenaline concentration did not change significantly in heifers that needed assistance, but increased during expulsion in heifers calving without help. In the goats, adrenaline and noradrenaline concentrations increased in association with expulsion of the first kid. The beta-endorphin concentration increased during labour in goats. In heifers that needed assistance, beta-endorphin concentration increased 1 h after labour but there was no change in heifers that did not need assistance. The met-enkephalin concentration was elevated during expulsion in heifers and fluctuated in the goats. Both oxytocin and vasopressin increased during expulsion in both groups of heifers, but vasopressin increased four times more in heifers needing assistance. In the goats, oxytocin reached its highest levels just as the feet of the first kid became visible, and vasopressin peaked as the head emerged. Parturition took longer in heifers that needed assistance than in those that did not. It is concluded that, even though the pattern of change differed between hormones during labour, the changes were related to the phases of labour. A longer labour therefore meant that the hormone concentrations stayed elevated for longer. Vasopressin reached high levels in goats and was the only hormone for which plasma concentrations were higher in heifers that needed assistance than in those that did not, indicating that this hormone is released in order to deal with the pain-related stress associated with labour.

Radiotelemetrically recorded blood pressure and heart rate changes in relation to plasma catecholamine levels during parturition in the conscious, unrestrained goat.

The aim of this study was to investigate the extent of sympathetic nervous system activation during parturition in four unrestrained goats. Chronically implanted radiotelemetry devices registered heart rate and arterial blood pressure around the clock and blood was sampled for determination of plasma adrenaline and noradrenaline concentrations before, during and after labour. Two goats delivered two kids after moderately intensive abdominal contractions. A third goat had dystocia, and was treated with prostaglandin F2 alpha. One normal kid and one mummified foetus were delivered manually. After milking, a third kid was born spontaneously. The fourth goat experienced severe abdominal contractions and delivered one kid. Mean blood pressure was 69 +/- 2 mmHg the day before parturition, increased gradually during the labour pains, and reached a maximal value of 120 +/- 7 mmHg when the head of the first kid was visible (P < or = 0.05). Heart rate was 134 +/- 4 beats min-1 the day before parturition and peaked when the first kid was born (159 +/- 6 beats min-1, P < or = 0.05), as did plasma adrenaline concentration (from 0.4 +/- 0.2 nmol L-1 to 2.7 +/- 1.2 nmol L-1, P < or = 0.05). The concentration of noradrenaline increased from 4.8 +/- 2.3 nmol L-1 to 12.2 +/- 8.4 nmol L-1 (P < or = 0.05), when the head of the first kid was visible. Expulsion of the second and third kids caused relatively smaller increases in blood pressure, heart rate and catecholamines than those seen with the first born kid. It is concluded that changes in pressure, heart rate and catecholamines during parturition are related to the different phases of labour and not to its duration or severity.

Parathyroid hormone-related protein in neonatal and reproductive goats determined by a sensitive time-resolved immunofluorometric assay.

OBJECTIVE: High concentrations of parathyroid hormone-related protein (PTHrP) have been found in goat milk but it is not known whether it can enter the circulation of the neonate. In this study we have developed a sensitive two-site lanthanide immunofluorometric assay (IFMA) using dissociation and enhancement time-resolved fluorometry to address this question. METHOD: Affinity-purified anti-PTHrP 38-67 raised in rabbit was biotinylated and immobilized in streptavidin-coated microtitration wells as a 'capture' antibody. As a signal, affinity-purified anti-PTHrP 1-34, raised in sheep, was labeled with an europium chelate. A sensitivity of 0.3 pmol/l was achieved. PTHrP levels were determined in the plasma of eleven neonatal, seven parturient and six non-pregnant, non-lactating goats as well as in goat milk. RESULTS: The circulating PTHrP levels (mean +/- S.D.) were significantly increased at day 1 (6.1 +/- 1.7 pmol/l: P < 0.01) and day 3 (3.5 +/- 0.6 pmol/l: P < 0.05) after birth in the male kids (n = 8) bottle-fed with milk from the dams, compared with before (2.2 +/- 0.7 pmol/l) and 30 min after (2.0 +/- 0.6 pmol/l) the first feeding and 14 days (2.4 +/- 0.8 pmol/l) later. In the female kids (n = 3) fed with formula there was no such increase and the concentrations remained between 1.6-1.9 pmol/l. In the parturient goats the mean +/- S.D. PTHrP levels before, during and after parturition were 2.9 +/- 1.7, 4.2 +/- 2.4 and 3.7 +/- 2.2 pmol/l respectively (n = 7) which demonstrated that plasma PTHrP was higher during and after parturition in comparison with before (P < 0.05). The levels in non-pregnant, non-lactating goats were 3.3 +/- 1.5 pmol/l (n = 6). PTHrP levels in goat milk were in the nanomolar range and were highest in the colostrum. CONCLUSIONS: A significant increase of plasma PTHrP was observed in goat kids fed with milk from their dams and this increase was not found in kids fed with formula. Plasma PTHrP was also increased during parturition.

The preference for warm drinking water induces hyperhydration in heat-stressed lactating goats.

The aim of this study was to determine whether lactating goats regulate their water intake when given a choice between warm (35 degrees C) and cold (15 degrees C) water. Six lactating goats were kept individually in pens. At 07.00 h one bucket was filled with warm water and another with cold water. Water consumption was measured at intervals until 18.00 h. Water temperature was not controlled at night. Two experiments were made, one at normal room temperature (18-19 degrees C) and the other at 39-40 degrees C from 10.45 to 17.00 h. At normal room temperature the goats drank 6.0 +/- 1.4 L of the warm water but only 1.7 +/- 1.3 L of the cold water (P < 0.001). The total water consumption, including the following night, was 9.2 +/- 1.6 L. This differed from control days, when only cold water was available (5.9 +/- 0.4 L; P < 0.05). Neither the plasma Na concentration nor the osmolality changed, which indicate that the goats regulated their fluid balance. In the experiments involving heat stress the goats drank 11.5 +/- 1.7 L of the warm water but only 2.0 +/- 1.0 L of the cold water (P < 0.001). In total, including the following night, they drank 16.2 +/- 2.6 L (P < 0.001 vs. control days). Goats drank repeatedly despite falling plasma Na and osmolality, proving that their thirst was not induced by water losses. Their abdomens became distended, indicating that water was stored in the reticulo-rumen and explaining why their body weights increased. The goats appeared distressed. It is concluded that goats prefer to drink warm water. Nevertheless, our results suggest that, if possible, warm drinking water should not be given to lactating goats under hot ambient conditions.

Reproductive period affects water intake in heat-stressed dehydrated goats.

Water intake following dehydration was studied in pregnant (N = 5), lactating (N = 4) and nonpregnant, nonlactating (N = 5) Swedish domestic goats (Capra hircus) to investigate if reproductive period affected drinking. Plasma cortisol concentration and the hematocrit value were measured to evaluate stress. The goats were water deprived from 9.00 h until 15.05 h the next day. They were fed at 7.00 and 15.20 h. On the second day, ambient temperature was increased from 20 degrees C to 38-39.5 degrees C for 5.15 h to accelerate water losses. Water temperature during rehydration was 35 +/- 1 degree C. Plasma Na concentration and osmolality increased most in dehydrated and heat-stressed pregnant and lactating goats. Pregnant goats lost 2.2 kg of their body weight. They drank 3.5 l immediately, followed by 2.5 l during afternoon eating. Lactating goats lost 4.9 kg and drank 6.3 l at once, and 3.9 l during feeding. Nonpregnant, nonlactating goats lost 1.7 kg and drank 2.6 l followed by 0.6 l. The large water consumption in pregnant and lactating goats caused hyponatremia and hemodilution, but they continued to drink during the night (0.5 +/- 0.2 l and 0.8 +/- 0.5 l, respectively). Renal free water clearance increased in all periods, with a long-lasting water diuresis during pregnancy. Plasma cortisol concentrations and the hematocrit values rose in connection with water intake. These results imply that the thirst center became less sensitive to inhibitory signals from the oropharyngeal tract and the diluted blood plasma during pregnancy and lactation. Catching sight of water was the most exciting procedure during these experiments.

A long term study of goats naturally infected with caprine arthritis-encephalitis virus.

The caprine arthritis-encephalitis virus (CAEV) is a big problem in dairy goat industry. Little is known about its characteristics in naturally infected goat herds. The aims of this study were: 1) to study how antibody expression, measured by agar gel immunodiffusion test (AGIDT), varied over time in naturally infected, seropositive goats, 2) to observe clinical signs in seropositive adult goats and 3) to follow seroconversion and gamma globulin concentration in goat kids artificially reared on cow milk replacement product only, compared to kids reared on untreated goat milk. The antibody expression pattern to the viral proteins gp135 and p28 varied in the individual goat and intermittent negative reactions were seen in 19 adult animals followed for 30-91 weeks. Four seropositive goats developed clinical symptoms with difficulties to move. However, no correlation between clinical signs and antibody expression pattern was seen. During the first 27 weeks of age no kid in the milk replacement reared group (N = 4) seroconverted, but 5 of the 7 kids fed goat milk occasionally showed a positive antibody reaction. The gamma globulin concentration was significantly higher in the goat milk fed group until the kids had become more than 19 weeks old. The results show that a great variation of the antibody pattern in individual goats occurs, and therefore the AGIDT is only reliable as a herd screening test. Frequent sampling is necessary to get reliable information about spreading of the CAEV in a naturally infected goat herd. Removing kids from their dams immediately after birth combined with segregation and artificial rearing protected them from CAEV infection. However their gamma globulin concentration was initially low.

Heat stress causes excessive drinking in fed and food deprived pregnant goats.

Water intake was studied in six goats during late pregnancy to investigate if heat stress induces primary polydipsia. The goats were fed twice daily or deprived of food for 24 hr to avoid postprandial drinking and induce hyponatremia. Signs of stress was evaluated by determination of plasma cortisol concentration. Ambient temperature was increased from 20 degrees C to 38.0-39.5 degrees C for 5.15 hr. Water temperature was 35 +/- 1 degree C during heat stress. Fed goats started to drink 13 +/- 3 min after the heaters were turned on and water diuresis developed in five of them. The body weight increased simultaneously with decreased plasma Na concentration and osmolality showing that the goats also stored water. During food deprivation, plasma Na concentration and osmolality fell, and plasma aldosterone concentration increased. Despite hyponatremia, food deprived goats started to drink 44 +/- 15 min after the heaters were turned on and then they drank repeatedly. Three of them underwent water diuresis. Hemodilution was observed both in fed and food-deprived goats during heat stress. These results show that heat stress induced primary polydipsia in pregnant goats. It is suggested that stimulating signals from warmth receptors overrode inhibiting influences from receptors signalling hyponatremia and hypoosmolality at the "thirst center" in the hypothalamus. Heat stress did not increase plasma cortisol concentration, but catching sight of food caused an abrupt, short-lasting elevation.

Intraruminal fluid administration to goats: effects of handling and fluid temperature.

Physiological stress response to intraruminal fluid administration was studied in 5 female goats. The fluid was given through a stomach tube. The water was cold (10 degrees C) or warm (38 degrees C) and in an amount of 7.5-10% of body weight. Plasma concentrations of adrenaline, noradrenaline and cortisol increased significantly after both treatments. Adrenaline and noradrenaline responses were greater and more longlasting after cold water administration, but there was no difference in cortisol response between cold and warm water. Haematocrit and plasma protein concentrations increased also and the effects of cold water lasted longer. Blood pressure showed a sharp rise of short duration and was independent of water temperature. The immediate tachycardiac response was similar with both treatments, but cold water caused an additional peak 15 min later. The cooling did not increase plasma histamine level. The results suggest that stomach intubation and administration of water into the rumen leads to strong activation of hormonal and cardiovascular stress parameters even in goats well adapted to handling. Low temperature of the fluid further heightens the effect. Warming of fluids to near body temperature before their administration is thus recommended.