Metabolic stress-dependent regulation of the mitochondrial biogenic molecular response to high-intensity exercise in human skeletal muscle.

KEY POINTS: Low-volume high-intensity exercise training promotes muscle mitochondrial adaptations that resemble those associated with high-volume moderate-intensity exercise training. These training-induced mitochondrial adaptations stem from the cumulative effects of transient transcriptional responses to each acute exercise bout. However, whether metabolic stress is a key mediator of the acute molecular responses to high-intensity exercise is still incompletely understood. Here we show that, by comparing different work-matched low-volume high-intensity exercise protocols, more marked metabolic perturbations were associated with enhanced mitochondrial biogenesis-related muscle mRNA responses. Furthermore, when compared with high-volume moderate-intensity exercise, only the low-volume high-intensity exercise eliciting severe metabolic stress compensated for reduced exercise volume in the induction of mitochondrial biogenic mRNA responses. The present results, besides improving our understanding of the mechanisms mediating exercise-induced mitochondrial biogenesis, may have implications for applied and clinical research that adopts exercise as a means to increase muscle mitochondrial content and function in healthy or diseased individuals. ABSTRACT: The aim of the present study was to examine the impact of exercise-induced metabolic stress on regulation of the molecular responses promoting skeletal muscle mitochondrial biogenesis. Twelve endurance-trained men performed three cycling exercise protocols characterized by different metabolic profiles in a randomized, counter-balanced order. Specifically, two work-matched low-volume supramaximal-intensity intermittent regimes, consisting of repeated-sprint (RS) and speed endurance (SE) exercise, were employed and compared with a high-volume continuous moderate-intensity exercise (CM) protocol. Vastus lateralis muscle samples were obtained before, immediately after, and 3 h after exercise. SE produced the most marked metabolic perturbations as evidenced by the greatest changes in muscle lactate and pH, concomitantly with higher post-exercise plasma adrenaline levels in comparison with RS and CM. Exercise-induced phosphorylation of CaMKII and p38 MAPK was greater in SE than in RS and CM. The exercise-induced PGC-1α mRNA response was higher in SE and CM than in RS, with no difference between SE and CM. Muscle NRF-2, TFAM, MFN2, DRP1 and SOD2 mRNA content was elevated to the same extent by SE and CM, while RS had no effect on these mRNAs. The exercise-induced HSP72 mRNA response was larger in SE than in RS and CM. Thus, the present results suggest that, for a given exercise volume, the initial events associated with mitochondrial biogenesis are modulated by metabolic stress. In addition, high-intensity exercise seems to compensate for reduced exercise volume in the induction of mitochondrial biogenic molecular responses only when the intense exercise elicits marked metabolic perturbations.

Systematic bias between running speed and metabolic power data in elite soccer players: influence of drill type.

The aims of the present study were to: i) evaluate the agreement between estimates of high-intensity activity during soccer small-sided games (SSGs) based on running speed alone and estimated metabolic power derived from a combination of running speed and acceleration; ii) evaluate whether any bias between the 2 approaches is dependent upon playing position or drill characteristics. 3 types of SSGs (5vs5, 7vs7 and 10vs10) were completed by 26 English Premier League outfield players. A total of 420 individual drill observations were collected over the in-season period using portable global positioning system technology. High-intensity activity was estimated using the total distance covered at speeds>14.4 km · h(-1) (TS) and the equivalent metabolic power threshold of > 20 W · kg(-1) (TP). We selected 0.2 as the minimally important standardised difference between methods. High-intensity demands were systematically higher (~100%, p<0.001) when expressed as TP vs. TS irrespective of playing position and SSG. The magnitude of this difference increased as the size of SSG decreased (p<0.01) with a difference of ~200% observed in the 5vs5 SSG. A greater difference between TP and TS was also evident in central defenders compared to other positions (p<0.05) particularly during the 5vs5 SSG (~350%). We conclude that the high-intensity demands of SSGs in elite soccer players are systematically underestimated by running speed alone particularly during "small" SSGs and especially for central defenders. Estimations of metabolic power provide a more valid estimation as to the true demands of SSGs.

Monitoring training in elite soccer players: systematic bias between running speed and metabolic power data.

We compared measurements of high-intensity activity during field-based training sessions in elite soccer players of different playing positions. Agreement was appraised between measurements of running speed alone and predicted metabolic power derived from a combination of running speed and acceleration. Data was collected during a 10-week phase of the competitive season from 26 English Premier League outfield players using global positioning system technology. High-intensity activity was estimated using the total distance covered at speeds >14.4 km · h⁻¹ (TS) and the equivalent metabolic power threshold of >20 W · kg⁻¹ (TP), respectively. We selected 0.2 as the -minimally important standardised difference between methods. Mean training session TS was 478±300 m vs. 727±338 m for TP (p<0.001). This difference was greater for central defenders (~ 85%) vs. wide defenders and attackers (~ 60%) (p<0.05). The difference between methods also decreased as the proportion of high-intensity distance within a training session increased (R2=0.43; p<0.001). We conclude that the high-intensity demands of soccer training are underestimated by traditional measurements of running speed alone, especially in training sessions or playing positions associated with less high-intensity activity. Estimations of metabolic power better inform the coach as to the true demands of a training session.

Speed endurance training is a powerful stimulus for physiological adaptations and performance improvements of athletes.

The present article reviews the physiological and performance effects of speed endurance training consisting of exercise bouts at near maximal intensities in already trained subjects. Despite a reduction in training volume, speed endurance training of endurance-trained athletes can maintain the oxidative capacity and improve intense short-duration/repeated high-intensity exercise performance lasting 30 s to 4 min, as it occurs in a number of sports. When combined with a basic volume of training including some aerobic high-intensity sessions, speed endurance training is also useful in enhancing performance during longer events, e.g. 40 K cycling and 10 K running. Athletes in team sports involving intense exercise actions and endurance aspects can also benefit from performing speed endurance training. These improvements don't appear to depend on changes in maximum oxygen uptake (VO2max), muscle substrate levels, glycolytic and oxidative enzymes activity, and membrane transport proteins involved in pH regulation. Instead they appear to be related to a reduced energy expenditure during submaximal exercise and a higher expression of muscle Na(+) ,K(+) pump α-subunits, which via a higher Na(+) ,K(+) pump activity during exercise may delay fatigue development during intense exercise. In conclusion, athletes from disciplines involving periods of intense exercise can benefit from the inclusion of speed endurance sessions in their training programs.

Effect of previous exhaustive exercise on metabolism and fatigue development during intense exercise in humans.

The present study examined how metabolic response and work capacity are affected by previous exhaustive exercise. Seven subjects performed an exhaustive cycle exercise ( approximately 130%-max; EX2) after warm-up (CON) and 2 min after an exhaustive bout at a very high (VH; approximately 30 s), high (HI; approximately 3 min) or low (LO; approximately 2 h) intensity. Compared with CON, performance during EX2 was reduced (P<0.05) more in HI and LO than in VH (61+/-4% and 68+/-3% vs 35+/-4%). The muscle glycogen before EX2 was lower (P<0.05) in LO than in HI and VH, but the muscle glycogen utilization rates during EX2 were not different. Muscle glycogen concentration before EX2 was related (P<0.05) to the mean rate of muscle glycogen utilization during EX2 in HI and VH, and the mean rate of muscle lactate accumulation in LO. In HI, muscle pH before EX2 was lower (P<0.05) compared with VH and LO, but the same in HI and VH at the end of EX2. In HI, muscle pH before and after EX2 was inversely related (P<0.05) to the decrease in EX2 performance. Thus, muscle glycogen availability and low muscle pH do not per se control but appear to affect the rate of glycogenolysis/glycolysis and fatigue development during a repeated high-intensity exercise lasting 1/2-2 min.

Physiological and performance effects of generic versus specific aerobic training in soccer players.

The aim of this study was to compare the effects of specific (small-sided games) vs. generic (running) aerobic interval training on physical fitness and objective measures of match performance in soccer. Forty junior players were randomly assigned to either generic (n=20) or specific (n=20) interval training consisting of 4 bouts of 4 min at 90-95 % of maximum heart rate with 3 min active rest periods, completed twice a week. The following outcomes were measured at baseline (Pre), after 4 weeks of pre-season training (Mid), and after a further 8 weeks of training during the regular season (Post): maximum oxygen uptake, lactate threshold (Tlac), running economy at Tlac, a soccer-specific endurance test (Ekblom's circuit), and indices of physical performance during soccer matches (total distance and time spent standing, walking, and at low- and high-intensity running speed). Training load, as quantified by heart rate and rating of perceived exertion, was recorded during all training sessions and was similar between groups. There were significant improvements in aerobic fitness and match performance in both groups of soccer players, especially in response to the first 4 weeks of pre-season training. However, no significant differences between specific and generic aerobic interval training were found in any of the measured variables including soccer specific tests. The results of this study showed that both small-sided games and running are equally effective modes of aerobic interval training in junior soccer players.