[Immunological detection of sphingomyelin exchange protein in tumors and in embryonic rat liver]. / Immunologicheskoe obnaruzhenie sfingomielinperenosiashchego belka v opukholiakh i embrional'noi pecheni krys.

The interaction of postmicrosomal supernatants from the Jensen sarcoma, sarcoma 45, nephroma RA, normal, regeherating and fetal rat livers with antisera to the sphingomyelin exchange protein isolated from hepatoma 27 was studied by double immunodiffusion. It was shown that this protein is present in comparable amounts in all the tumours tested and in fetal rat liver, whereas in normal and regenerating liver its content is negligible. Based on the correlation between the content of sphingomyelin exchange protein in cell cytosols and the amount of sphingomyelin in the corresponding mitochondria it is suggested that this protein is responsible for the presence of sphingomyelin in mitochondria.

[Immunohistochemical study of cardiolipin and phosphatidylinositol in mouse liver]. / Immunogistokhimicheskoe izuchenie kardiolipina i fosfatidilinozita v pecheni myshi.

Localization of two phospholipid haptens--cardiolipin and phosphatidylinositol--in mouse liver sections was studied by the indirect method of fluorescent antibodies. Two types of liver sections--paraffin and cryostat, and two type of fixation--in acetone, and in the acetone, buffer, and formalin mixture--were used. Antiphospholipid sera stain specifically the plasma membrane of hepatocytes and predominantly the membrane region overlooking the blood capillary. A possibility of detecting the specific phospholipid haptens depends on the method of obtaining the sections and their fixation. Two types of immunization give two types of antiphospholipid sera which differ by the stability, by the possibility of monospecific antibodies isolation from them on lipid immunosorbents, and by the types of liver section staining.

[Antigenic structure of the plasma membranes of mouse liver cells. III. Lipid haptens]. / Antigennaia struktura plazmaticheskikh membran kletok pecheni myshi. III. Lipidnye gapteny.

The total glycolipids, gangliosides, neutral lipids and some individual lipids of these fractions were isolated from the whole lipid extract of the mouse liver cell suspension. The antigenic activity of the isolated lipids was studied with the complement fixation test using the rabbit antisera against the liver cell ghosts. Active lipids were: glycosphingolipids, gangliosides, cardiolipin and fraction containing polar glyco- and phospholipid mixture. The cardiolipins isolated from animal cells of some species differed in their antigenic activity. With the immunofluorescence method, the absorbtion of antighost sera by lipid haptens resulted in a decreased fluorescence of plasma membranes of cells in the liver sections. This fact may evidence for the membrane localization of the studied haptens.