ABSTRACT
Feasibility of manual-assisted laparoscopic surgery in the treatment of coloproctologic diseases was designated. The investigation included 25 patients operated on for diseases of the colon. In 11 of them made manual-assisted laparoscopic surgery. Length of cut with manual-assisted laparoscopic interventions in 3.8 times less conventional laparotomy section, the total blood loss was 2.3 times less than in open surgery. Duration of patients rehabilitation after such surgery is 1.8 times less than after laparotomy. Laparoscopic surgery of the hand asystens in coloproctology mini-invasive is, however, retain the advantages of open surgery. At the present stage of coloproctology are, on the one hand, is the first step to classic laparoscopic surgery on the other--an alternative to conversion.
Subject(s)
Colon/surgery , Colonic Neoplasms/surgery , Diverticulum, Colon/surgery , Hand-Assisted Laparoscopy , Aged , Colon/pathology , Colonic Neoplasms/pathology , Diverticulum, Colon/pathology , Female , Humans , Male , Middle Aged , Treatment OutcomeABSTRACT
The investigation of interaction of 7-aminoactinomycin D with DNA is hampered at nanomolar concentrations by low quantum yield of the dye fluorescence and high adsorbability of the dye. It was found that, instead of the increase in the fluorescence of 7-aminoactinomycin D after binding with DNA, a decrease in the fluorescence takes place, because 7-aminoactinomycin D complexed with DNA exists in two states: photochemically active and inactive. The presence of the photochemically active state of 7-aminoactinomycin D causes an apparent increase in the diffusion coefficient upon embedding of 7-aminoactinomycin D into DNA. The data obtained allow one to state that 7-aminoactinomycin D: (1) forms dimers at micromolar concentration, (2) has two specific photodestruction times, and (3) binds with DNA more effectively than actinomycin D. Polarization measurements made it possible to estimate numerically the adsorption of 7-aminoactinomycin D on the walls of the measuring cell.
Subject(s)
DNA/chemistry , Dactinomycin/analogs & derivatives , Dactinomycin/chemistry , Dactinomycin/radiation effects , Dimerization , Lasers , Nucleic Acid Conformation , Oligonucleotides/chemistry , Solutions , Spectrometry, FluorescenceABSTRACT
The results of the study of the temperature dependence of water absorption spectra in the visible region and in the region of vibrational overtones are discussed.
