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1.
Sud Med Ekspert ; 46(5): 36-41, 2003.
Article in Russian | MEDLINE | ID: mdl-14584413

ABSTRACT

The results of applying molecular-and-genetic markers of chromosomal DNA in identifying unrecognizable remains of servicemen lost as dead in 1994-1996 and 1999-2001 military campaigns in the Northern Caucuses are summarized in the paper. Some of the specific features related with enzymatic amplification typing of DNA preparations sampled from degraded biological tissues of strongly deformed or decayed cadavers were analyzed. The typing results were analyzed by the AB0 system of degraded expertise samples in order to check the reliability of routine forensic-biological examinations as applicable to cadaver tissues with pronounced putrefactive changes. It was established that group-specific antigens of the AB0 system were correctly determined only in 67.5% of cases. False results were obtained in the other 32.5% of cases. Most of them were related with determination of groups 0(I) and A(II).


Subject(s)
Chromosomes/genetics , DNA/analysis , Forensic Medicine , Military Personnel , Molecular Biology/methods , Warfare , Biomarkers/analysis , DNA/genetics , HLA-D Antigens/genetics , Humans , Polymerase Chain Reaction , Polymorphism, Genetic , Postmortem Changes , Russia
2.
Sud Med Ekspert ; 45(5): 12-4, 2002.
Article in Russian | MEDLINE | ID: mdl-12516268

ABSTRACT

Until recently, data bases on incidence rates of alleles STR (LPL, vWA and THO1 loci) have not been introduced in Russia. To obtain information on the above rates in Russia, a large scale research of distribution of LPL, vWA and Tho1 loci has been performed among a sample of 442 blood-unrelated persons from 57 regions of the Russian Federation to specify basic assessment characteristics of these loci and to raise efficiency of expert use of identifying systems on their base. Relevant parameters (allele frequencies) are presented for each polymorphic locus as well as basic population characteristics of polymorphism in the studied sample of RF population. These results can be used as key parameters for standard probability estimations in assessing the results of molecular-genetic identification.


Subject(s)
Forensic Medicine , Genetics, Population/methods , Microsatellite Repeats/genetics , Polymorphism, Genetic , Alleles , DNA/analysis , Humans , Polymerase Chain Reaction , Russia
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