ABSTRACT
Sorption isotherms of pancreatic lipase on solid supports were studied. It was shown that the enzyme adsorption can be described by Langmuir equation for hydrophobic surface and by the equation which takes into account reversible dimerization of the protein in the absorption layer for hydrophilic surface. The catalytic properties of adsorbed lipase depend on the nature of solid support. The significant role of the structure of adsorption layer in heterogeneous activation of the enzyme on hydrophobic surface was suggested.
Subject(s)
Lipase/analysis , Lipolysis , Adsorption , Animals , In Vitro Techniques , Kinetics , Models, Biological , Pancreas/enzymology , SwineABSTRACT
Experimental hemophthalmia was made by injecting auto-blood (0.2-1 ml) into the vitreous body of the rabbit eye. The structure of hematoma was investigated at light and electron microscopic levels. It was observed that blood being in contact with the vitreous body formed an isolated focus (hematoma) containing all the elements inherent to the thrombus: red cells, platelets, fibrin, and white blood cells. Mass hemolysis of red cells, and destruction of platelets occurred in the central zone of hematoma on the 1st-3rd day. From the moment of blood contact with the vitreous body, a membrane-like layer was demonstrable on the hematoma surface, favouring relative stabilization of hematoma structure.
Subject(s)
Hematoma/pathology , Vitreous Body/ultrastructure , Animals , Eye Diseases/pathology , Hemolysis , RabbitsSubject(s)
Membrane Proteins , Membranes, Artificial , Serum Albumin , Animals , Cattle , Humans , Hydrogen-Ion Concentration , Serum Albumin, BovineABSTRACT
Structure changes of ganglioside micelles in the process of serotonin bonding are considered. It was shown by viscosimetric and differential refraction studies that stretching of elliptical ganglioside micelles in the serotonin bonding process took place. The scope of micelles form changes depends on the pH value. An increase of the micelles summary negative charge at pH 9 increased the number of bonding centres and simultaneously caused deeper deformation of micelles due to the repulsion of the identically charged groups.
